1St BASE Custom Pab Production Order Form

Date:

Name of Researcher: Email:
Address: Contact No.
Purchase Order Number: Project Name:
Please choose preferred package: PAb 8-week Package 1, 2, 3, 4 (see overleaf for details)
Immunogen Details:
*Immunogen provided by Researcher (for Package 1 only)
Protein / Peptide Size: kDa / Amount: mg
Form of Protein:
Solution (Concentration: mg/ml) (Buffer contains: ) / Lyophilised Gel Strip
* Note: 4 mg of soluble Immunogen required for Affinity Purification and 1 mg for each ELISA Testing (2 animals)
Additional Services:
Conjugation of Immunogen to KLH carrier protein
Note: 3mg of antigen in 1xPBS required (min. of 1mg/ml)
Peptide Synthesized by 1st BASE (included in Package 2, 3 and 4)
Note:
a) FREE up to 15 residues, > 70% purity for Package 2, 3 and 4
b) Recommended < 30 residues for other Custom Package
c) Allow 2 weeks of synthesis / Sequence: NH2 - -OH
Optional purification of peptides:
Purity > 80% by HPLC (charges applied)
Purity > 95% by HPLC (charges applied)
Shipment Information: / Use 1st BASE’s Fedex Account / Charge to my FedEx Account (No. )
Preference:
Ship 2ml Aliquots of Bleeds 1 & 2 (note: additional charges may apply)
Ship after Bleed 2 & at end of project (total of 2 shipments) (note: additional charges may apply)
Upon Project Completion

Custom PAb Packages and Services

Please Tick / Package / Details / Remarks / Please Tick / Additional Services: / Details / Remarks
PAb 8-week Package 1 / Polyclonal Antibody Production - 2 Rabbits
(*Immunogen provided by researcher)
Includes:
-Immunization and anti-sera production (75ml/rabbit)
-ELISA Testing for Bleed 1 / *2mg of antigen in 1xPBS preferred (min. of 1mg/ml) / ELISA Testing and Reporting (additional) / ELISA Testing and Reporting, on top of
ELISA on Bleed 1 (which already included in
Packages 1 to 4)
Please indicate which Bleed? 2, 3 / 0.4mg soluble antigen per ELISA
PAb 8-week Package 2 / Polyclonal Antibody Production - 2 Rabbits
Includes:
-Peptide synthesis (15aa), Purification (> 70% Purity)
and Conjugation
-Immunization and anti-sera production (75ml/rabbit)
-ELISA Testing for Bleed 1 / Contact us for a free peptide antigenicity analysis! / Protein A / G Purification (25ml) / Protein A/G Purification (25ml of purified
anti-sera).
Please state which bleed? 1, 2, 3
PAb 8-week Package 3 / Polyclonal Antibody Production - 2 Rabbits
Includes:
-Peptide synthesis (15aa), Purification (> 70% Purity)
and Conjugation
-Immunization and anti-sera production (62.5mL/rabbit)
-Affinity Purification on Bleed 3 for 12.5mL/ rabbit
(includes reusable column)
-ELISA Testing on Bleed 1 / Affinity Purification (25ml) / Affinity Column Preparation and
Purification of Bleeds (25ml of purified
anti-sera) .
Please state which bleed? 1, 2, 3 / Min. 3mg of soluble antigen required
PAb 8-week Package 4 / Polyclonal Antibody Production - 2 Rabbits
Includes:
-Peptide synthesis, Purification (> 70% Purity)
and Conjugation
- Immunization and anti-sera production
- Large scale Affinity Purification for all Bleeds (75mL/ rabbit)
- ELISA Testing on Bleed 1 / Large scale Affinity Purification / Large scale Affinity Column Preparation
and Purification (150-200ml of purified
serum)
Rabbit Exsanguination / Exsanguination of Animals for Project
Termination
(Terminal bleed will be about 60ml
per animal)
Project Extension / Project Extension (28 days) includes
-1 Additional Boost and
-2 Bleeds (25ml each) for 2 rabbits
Custom Antibody Screening-IHC / Screening of PAb serum on a choice of
IHC tissue panel.
Custom Antibody Screening-WB / Screening of PAb serum on choice of
14 Western Blot lysates

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