The Wiltshire School of Beauty and Holistic Therapy Certificate of Merit in Anatomy and Physiology©
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Development and Maintenance of Epidermal Stem Cells in Skin Adnexa
International Journal of Molecular Sciences Review Development and Maintenance of Epidermal Stem Cells in Skin Adnexa Jaroslav Mokry * and Rishikaysh Pisal Medical Faculty, Charles University, 500 03 Hradec Kralove, Czech Republic; [email protected] * Correspondence: [email protected] Received: 30 October 2020; Accepted: 18 December 2020; Published: 20 December 2020 Abstract: The skin surface is modified by numerous appendages. These structures arise from epithelial stem cells (SCs) through the induction of epidermal placodes as a result of local signalling interplay with mesenchymal cells based on the Wnt–(Dkk4)–Eda–Shh cascade. Slight modifications of the cascade, with the participation of antagonistic signalling, decide whether multipotent epidermal SCs develop in interfollicular epidermis, scales, hair/feather follicles, nails or skin glands. This review describes the roles of epidermal SCs in the development of skin adnexa and interfollicular epidermis, as well as their maintenance. Each skin structure arises from distinct pools of epidermal SCs that are harboured in specific but different niches that control SC behaviour. Such relationships explain differences in marker and gene expression patterns between particular SC subsets. The activity of well-compartmentalized epidermal SCs is orchestrated with that of other skin cells not only along the hair cycle but also in the course of skin regeneration following injury. This review highlights several membrane markers, cytoplasmic proteins and transcription factors associated with epidermal SCs. Keywords: stem cell; epidermal placode; skin adnexa; signalling; hair pigmentation; markers; keratins 1. Epidermal Stem Cells as Units of Development 1.1. Development of the Epidermis and Placode Formation The embryonic skin at very early stages of development is covered by a surface ectoderm that is a precursor to the epidermis and its multiple derivatives. -
Diapositiva 1
Ingegneria delle tecnologie per la salute Fondamenti di anatomia e istologia Apparato tegumentario aa. 2017-18 INTEGUMENTARY SYSTEM integumentary system = refers to skin and its accessory structures responsible for much more than simply human outward appearance: about 16% of body weight, covering an area of 1.5 to 2 m2 (= largest organ system in human body). • skin protects inner organs INTEGUMENTARY SYSTEM • skin = even not typical, but an organ, made of tissues that work together as a single structure to perform unique and critical functions • integumentary system = skin + its accessory structures, providing body with overall protection. • made of multiple layers of cells and tissues, which are held to underlying structures by connective tissue: deeper layer of skin is well vascularized (has numerous blood vessels) and also has numerous sensory, and autonomic and sympathetic nerve fibers ensuring communication to and from brain. INTEGUMENTARY SYSTEM Overview • Largest organ (15% of body weight) • Epidermis – keratinized stratified squamous epithelium • Dermis – connective tissue layer • Hypodermis • Thickness variable, normally 1-2 mm – dermis may thicken, up to 6 mm – stratum corneum layer increased • calluses on hands and feet Structure of the Skin 2 layers: epidermis + dermis SKIN: histology SKIN: histology SKIN: histology Cells of the Epidermis • Stem cells – undifferentiated cells in deepest layers • Keratinocytes – most of the skin cells • Melanocytes – synthesize pigment that shield UV • Tactile (merkel) cells – receptor cells associated with nerve fibers • Dendritic (langerhans) cells – macrophages guard against pathogens Cell and Layers of the Epidermis Epidermis: histology = composed of keratinized, stratified squamous epithelium, made of 4 or 5 layers of epithelial cells, depending on its location in body. -
Nail Anatomy and Physiology for the Clinician 1
Nail Anatomy and Physiology for the Clinician 1 The nails have several important uses, which are as they are produced and remain stored during easily appreciable when the nails are absent or growth. they lose their function. The most evident use of It is therefore important to know how the fi ngernails is to be an ornament of the hand, but healthy nail appears and how it is formed, in we must not underestimate other important func- order to detect signs of pathology and understand tions, such as the protective value of the nail plate their pathogenesis. against trauma to the underlying distal phalanx, its counterpressure effect to the pulp important for walking and for tactile sensation, the scratch- 1.1 Nail Anatomy ing function, and the importance of fi ngernails and Physiology for manipulation of small objects. The nails can also provide information about What we call “nail” is the nail plate, the fi nal part the person’s work, habits, and health status, as of the activity of 4 epithelia that proliferate and several well-known nail features are a clue to sys- differentiate in a specifi c manner, in order to form temic diseases. Abnormal nails due to biting or and protect a healthy nail plate [1 ]. The “nail onychotillomania give clues to the person’s emo- unit” (Fig. 1.1 ) is composed by: tional/psychiatric status. Nail samples are uti- • Nail matrix: responsible for nail plate production lized for forensic and toxicology analysis, as • Nail folds: responsible for protection of the several substances are deposited in the nail plate nail matrix Proximal nail fold Nail plate Fig. -
Enabling Sweat-Based Biosensors: Solving the Problem of Low
Enabling sweat-based biosensors: Solving the problem of low biomarker concentration in sweat A dissertation submitted to the Graduate School of the University of Cincinnati in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biomedical Engineering of the College of Engineering & Applied Science by Andrew J. Jajack B.S., Biology, Wittenberg University, 2014 Committee Chairs: Jason C. Heikenfeld, Ph.D. and Chia-Ying Lin, Ph.D. Abstract Non-invasive, sweat biosensing will enable the development of an entirely new class of wearable devices capable of assessing health on a minute-to-minute basis. Every aspect of healthcare stands to benefit: prevention (activity tracking, stress-level monitoring, over-exertion alerting, dehydration warning), diagnosis (early-detection, new diagnostic techniques), and management (glucose tracking, drug-dose monitoring). Currently, blood is the gold standard for measuring the level of most biomarkers in the body. Unlike blood, sweat can be measured outside of the body with little inconvenience. While some biomarkers are produced in the sweat gland itself, most are produced elsewhere and must diffuse into sweat. These biomarkers come directly from blood or interstitial fluid which surrounds the sweat gland. However, a two-cell thick epithelium acts as barrier and dilutes most biomarkers in sweat. As a result, many biomarkers that would be useful to monitor are diluted in sweat to concentrations below what can be detected by current biosensors. This is a core challenge that must be overcome before the advantages of sweat biosensing can be fully realized. The objective of this dissertation is to develop methods of concentrating biomarkers in sweat to bring them into range of available biosensors. -
Biomechanics of Human Stratum Corneum: Dry Skin Conditions, Tissue Damage and Alleviation a Dissertation Submitted to the Depar
BIOMECHANICS OF HUMAN STRATUM CORNEUM: DRY SKIN CONDITIONS, TISSUE DAMAGE AND ALLEVIATION A DISSERTATION SUBMITTED TO THE DEPARTMENT OF MATERIALS SCIENCE AND ENGINEERING AND THE COMMITTEE ON GRADUATE STUDIES OF STANFORD UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY Kemal Levi November 2009 © 2010 by Kemal Levi. All Rights Reserved. Re-distributed by Stanford University under license with the author. This work is licensed under a Creative Commons Attribution- Noncommercial 3.0 United States License. http://creativecommons.org/licenses/by-nc/3.0/us/ This dissertation is online at: http://purl.stanford.edu/cb644mw1707 ii I certify that I have read this dissertation and that, in my opinion, it is fully adequate in scope and quality as a dissertation for the degree of Doctor of Philosophy. Reinhold Dauskardt, Primary Adviser I certify that I have read this dissertation and that, in my opinion, it is fully adequate in scope and quality as a dissertation for the degree of Doctor of Philosophy. Sarah Heilshorn I certify that I have read this dissertation and that, in my opinion, it is fully adequate in scope and quality as a dissertation for the degree of Doctor of Philosophy. William Nix Approved for the Stanford University Committee on Graduate Studies. Patricia J. Gumport, Vice Provost Graduate Education This signature page was generated electronically upon submission of this dissertation in electronic format. An original signed hard copy of the signature page is on file in University Archives. iii Abstract The outermost layer of human skin, the stratum corneum (SC), is subject daily to variable ambient moisture and temperature conditions as well as application of potentially damaging cleansing agents. -
The Integumentary System
CHAPTER 5: THE INTEGUMENTARY SYSTEM Copyright © 2010 Pearson Education, Inc. OVERALL SKIN STRUCTURE 3 LAYERS Copyright © 2010 Pearson Education, Inc. Figure 5.1 Skin structure. Hair shaft Dermal papillae Epidermis Subpapillary vascular plexus Papillary layer Pore Appendages of skin Dermis Reticular • Eccrine sweat layer gland • Arrector pili muscle Hypodermis • Sebaceous (oil) gland (superficial fascia) • Hair follicle Nervous structures • Hair root • Sensory nerve fiber Cutaneous vascular • Pacinian corpuscle plexus • Hair follicle receptor Adipose tissue (root hair plexus) Copyright © 2010 Pearson Education, Inc. EPIDERMIS 4 (or 5) LAYERS Copyright © 2010 Pearson Education, Inc. Figure 5.2 The main structural features of the skin epidermis. Keratinocytes Stratum corneum Stratum granulosum Epidermal Stratum spinosum dendritic cell Tactile (Merkel) Stratum basale Dermis cell Sensory nerve ending (a) Dermis Desmosomes Melanocyte (b) Melanin granule Copyright © 2010 Pearson Education, Inc. DERMIS 2 LAYERS Copyright © 2010 Pearson Education, Inc. Figure 5.3 The two regions of the dermis. Dermis (b) Papillary layer of dermis, SEM (22,700x) (a) Light micrograph of thick skin identifying the extent of the dermis, (50x) (c) Reticular layer of dermis, SEM (38,500x) Copyright © 2010 Pearson Education, Inc. Figure 5.3a The two regions of the dermis. Dermis (a) Light micrograph of thick skin identifying the extent of the dermis, (50x) Copyright © 2010 Pearson Education, Inc. Q1: The type of gland which secretes its products onto a surface is an _______ gland. 1) Endocrine 2) Exocrine 3) Merocrine 4) Holocrine Copyright © 2010 Pearson Education, Inc. Q2: The embryonic tissue which gives rise to muscle and most connective tissue is… 1) Ectoderm 2) Endoderm 3) Mesoderm Copyright © 2010 Pearson Education, Inc. -
Nomina Histologica Veterinaria, First Edition
NOMINA HISTOLOGICA VETERINARIA Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association of Veterinary Anatomists Published on the website of the World Association of Veterinary Anatomists www.wava-amav.org 2017 CONTENTS Introduction i Principles of term construction in N.H.V. iii Cytologia – Cytology 1 Textus epithelialis – Epithelial tissue 10 Textus connectivus – Connective tissue 13 Sanguis et Lympha – Blood and Lymph 17 Textus muscularis – Muscle tissue 19 Textus nervosus – Nerve tissue 20 Splanchnologia – Viscera 23 Systema digestorium – Digestive system 24 Systema respiratorium – Respiratory system 32 Systema urinarium – Urinary system 35 Organa genitalia masculina – Male genital system 38 Organa genitalia feminina – Female genital system 42 Systema endocrinum – Endocrine system 45 Systema cardiovasculare et lymphaticum [Angiologia] – Cardiovascular and lymphatic system 47 Systema nervosum – Nervous system 52 Receptores sensorii et Organa sensuum – Sensory receptors and Sense organs 58 Integumentum – Integument 64 INTRODUCTION The preparations leading to the publication of the present first edition of the Nomina Histologica Veterinaria has a long history spanning more than 50 years. Under the auspices of the World Association of Veterinary Anatomists (W.A.V.A.), the International Committee on Veterinary Anatomical Nomenclature (I.C.V.A.N.) appointed in Giessen, 1965, a Subcommittee on Histology and Embryology which started a working relation with the Subcommittee on Histology of the former International Anatomical Nomenclature Committee. In Mexico City, 1971, this Subcommittee presented a document entitled Nomina Histologica Veterinaria: A Working Draft as a basis for the continued work of the newly-appointed Subcommittee on Histological Nomenclature. This resulted in the editing of the Nomina Histologica Veterinaria: A Working Draft II (Toulouse, 1974), followed by preparations for publication of a Nomina Histologica Veterinaria. -
Sweat Glands • Oil Glands • Mammary Glands
Chapter 4 The Integumentary System Lecture Presentation by Steven Bassett Southeast Community College © 2015 Pearson Education, Inc. Introduction • The integumentary system is composed of: • Skin • Hair • Nails • Sweat glands • Oil glands • Mammary glands © 2015 Pearson Education, Inc. Introduction • The skin is the most visible organ of the body • Clinicians can tell a lot about the overall health of the body by examining the skin • Skin helps protect from the environment • Skin helps to regulate body temperature © 2015 Pearson Education, Inc. Integumentary Structure and Function • Cutaneous Membrane • Epidermis • Dermis • Accessory Structures • Hair follicles • Exocrine glands • Nails © 2015 Pearson Education, Inc. Figure 4.1 Functional Organization of the Integumentary System Integumentary System FUNCTIONS • Physical protection from • Synthesis and storage • Coordination of immune • Sensory information • Excretion environmental hazards of lipid reserves response to pathogens • Synthesis of vitamin D3 • Thermoregulation and cancers in skin Cutaneous Membrane Accessory Structures Epidermis Dermis Hair Follicles Exocrine Glands Nails • Protects dermis from Papillary Layer Reticular Layer • Produce hairs that • Assist in • Protect and trauma, chemicals protect skull thermoregulation support tips • Nourishes and • Restricts spread of • Controls skin permeability, • Produce hairs that • Excrete wastes of fingers and supports pathogens prevents water loss provide delicate • Lubricate toes epidermis penetrating epidermis • Prevents entry of -
Nails Develop from Thickened Areas of Epidermis at the Tips of Each Digit Called Nail Fields
Nail Biology: The Nail Apparatus Nail plate Proximal nail fold Nail matrix Nail bed Hyponychium Nail Biology: The Nail Apparatus Lies immediately above the periosteum of the distal phalanx The shape of the distal phalanx determines the shape and transverse curvature of the nail The intimate anatomic relationship between nail and bone accounts for the bone alterations in nail disorders and vice versa Nail Apparatus: Embryology Nail field develops during week 9 from the epidermis of the dorsal tip of the digit Proximal border of the nail field extends downward and proximally into the dermis to create the nail matrix primordium By week 15, the nail matrix is fully developed and starts to produce the nail plate Nails develop from thickened areas of epidermis at the tips of each digit called nail fields. Later these nail fields migrate onto the dorsal surface surrounded laterally and proximally by folds of epidermis called nail folds. Nail Func7on Protect the distal phalanx Enhance tactile discrimination Enhance ability to grasp small objects Scratching and grooming Natural weapon Aesthetic enhancement Pedal biomechanics The Nail Plate Fully keratinized structure produced throughout life Results from maturation and keratinization of the nail matrix epithelium Attachments: Lateral: lateral nail folds Proximal: proximal nail fold (covers 1/3 of the plate) Inferior: nail bed Distal: separates from underlying tissue at the hyponychium The Nail Plate Rectangular and curved in 2 axes Transverse and horizontal Smooth, although -
There Are 25 Questions, Each Worth 3 Points and a Short Essay Worth 25 Points. DO YOUR OWN WORK !! Use Your Time Wisely 1. T
Mr. Holder Integumentary System – Unit 5 December 3, 2015 ARC TEST 1.0 DO NOT MARK OR WRITE ON THIS QUIZ !! There are 25 questions, each worth 3 points and a short essay worth 25 points. DO YOUR OWN WORK !! Use Your Time Wisely 1. The Integumentary System is divided into how many layers? a) 2 b) 3 c) 4 d) 6 2. What are the two major groups of membranes covering the human body? a) Epithelial & Mucus b) Cutaneous & Mucus c) Epithelial & Connective Tissue d) None of these 3. Which internal membrane provides protection for your joints? a) Serous b) Synovial c) Cutaneous d) Mucus 4. These membranes line internal cavities exposed to air & excrete a gooey substance. a) Serous b) Synovial c) Cutaneous d) Mucus 5. The Integumentary System protects the human body from … a) Friction b) Hot & Cold Temperature c) Bacteria d) All of These 6. Which stratum of the epidermis is full of keratin, cornified to prevent water loss? a) Basale b) Granulosum c) Corneum d) None of These 7. Which body system extends into the dermis to provide information to your brain? a) Cardiovascular b) Immune c) Integumentary d) Nervous 8. Subcutaneous tissue includes adipose tissue or fat. It is also known as the … a) Dermis b) Papillary Layer c) Hypodermis d) Reticular Layer 9. The dermis is divided into two layers. Which of these is the thickest? a) Papillary b) Reticular c) Basale d) Hypodermis 10. Which stratum of the epidermis is responsible for new cell production? a) Corneum b) Basale c) Granulosum d) Spinosum Page 1 Mr. -
Curling Cuticles of the Great Toenails: a Case Report of Eponychogryphosis
Open Access Case Report DOI: 10.7759/cureus.3959 Curling Cuticles of the Great Toenails: A Case Report of Eponychogryphosis Philip R. Cohen 1 1. Dermatology, San Diego Family Dermatology, San Diego, USA Corresponding author: Philip R. Cohen, [email protected] Abstract The cuticle, also referred to as the eponychium, creates a seal between the proximal nail fold and the nail plate. It is derived from both the ventral and dorsal portions of the proximal nail fold. In addition to its principle function as a barrier preventing allergens, irritants and pathogens from entering the nail cul-de- sac, the cuticle can play a role as a model for evaluating the etiology and management of diseases that affect capillary microcirculation, provide a source of solid tissue for genetic disorder studies, and aid in the evaluation of patients in whom the diagnoses of either systemic scleroderma or dermatomyositis is being entertained. Curling cuticle is a distinctive and unique occurrence. The clinical features of a man with curling cuticles on the lateral portion of both great toes is described. Although a deficiency in personal hygiene may partially account for the clinical finding, the pathogenesis of this observation remains to be established. The term ‘eponychogryphosis’ is proposed to describe the alteration of the patient’s cuticles. Categories: Dermatology, Internal Medicine, Rheumatology Keywords: curl, curling, cuticle, eponychium, eponychogryphosis, fold, great, onychogryphosis, nail, toe Introduction The cuticle, also known as the eponychium, is an extension of the stratum corneum from the proximal nail fold [1-3]. It forms a seal that prevents allergens, irritants, and pathogens from entering the potential space between the distal skin of the digit and the nail plate [4-5]. -
Basic Biology of the Skin 3
© Jones and Bartlett Publishers, LLC. NOT FOR SALE OR DISTRIBUTION CHAPTER Basic Biology of the Skin 3 The skin is often underestimated for its impor- Layers of the skin: tance in health and disease. As a consequence, it’s frequently understudied by chiropractic students 1. Epidermis—the outer most layer of the skin (and perhaps, under-taught by chiropractic that is divided into the following fi ve layers school faculty). It is not our intention to present a from top to bottom. These layers can be mi- comprehensive review of anatomy and physiol- croscopically identifi ed: ogy of the skin, but rather a review of the basic Stratum corneum—also known as the biology of the skin as a prerequisite to the study horny cell layer, consisting mainly of kera- of pathophysiology of skin disease and the study tinocytes (fl at squamous cells) containing of diagnosis and treatment of skin disorders and a protein known as keratin. The thick layer diseases. The following material is presented in prevents water loss and prevents the entry an easy-to-read point format, which, though brief of bacteria. The thickness can vary region- in content, is suffi cient to provide a refresher ally. For example, the stratum corneum of course to mid-level or upper-level chiropractic the hands and feet are thick as they are students and chiropractors. more prone to injury. This layer is continu- Please refer to Figure 3-1, a cross-sectional ously shed but is replaced by new cells from drawing of the skin. This represents a typical the stratum basale (basal cell layer).