Table S1 Primer Sequences of Porcine STAT4 and STAT6 Genes Used in This Study

Table S1 primer sequences of porcine STAT4 and STAT6 genes used in this study

Gene name / Primer name / Primer sequence（5’-3’） / Binding region / Tm(℃) / Size(bp)
STAT4 / CDS-4F / ACTTGAGCACTGCCTGGGACC / 5’-UTR / 62.2 / 2476
CDS-4R / TTTTTAGCATTTCCGTCACAACA / 3’-UTR
STAT6 / CDS-6F / AGGGAGAAGACAGCAGAGGGG / 5’-UTR / 66.5 / 3890
CDS-6R / ATGGGCAAGTGTCCAGAGCAG / 3’-UTR
STAT4 / exp-4F / GGTTGTCTGCTCTACCATTCGCT / Exon21 / 66 / 221
exp-4R / TCGGATTGTTGAGATGGGGA / Exon22
STAT6 / exp-6F / CCCAGGTACTGAAGACGCAGAC / Exon9 / 60 / 254
exp-6R / CAGGTTCTTGAACAGGGCAGAG / Exon10
Beta-actin / ACTIN-F / GGACTTCGAGCAGGAGATGG / 62 / 233
ACTIN-R / GCACCGTGTTGGCGTAGAGG
STAT4 / map-4F / TTGTCTGCTCTACCATTCGCTG / Exon21 / 60 / 348
map-4R / TGAAGTCTGGTCACAGCTATGAA / Intron21
STAT6 / map-6F / TTTGGTCTCTTCTGTCCACTCC / Intron19 / 64 / 150
map-6R / CACCCTTCACAATAATCCTCTG / Intron19
STAT4 / pEGFP-4F / CCGCTCGAGAGCATGTCTCAGTGGAATCA / 5’-UTR / 60 / 2267
pEGFP-4R / CCGGAATTCCTTCAGCAGAATAGGGAGACT / 3’-UTR
STAT6 / pEGFP-6F / GCGAGCGCTATCATGTCTCTGTGGGGTCT / 5’-UTR / 60 / 2566
pEGFP-6R / CCGGAATTCGCCAACTGGGGTTAGCCCTTA / 3’-UTR
STAT6 / SNP-6F / AGGAGACACTGGGTAGGAGGAG / 3’-UTR / 62 / 276
SNP-6R / GTCACACAGGCAAAATCAGAGAG / 3’-UTR

Table S2 The exon/intron organizations of porcine STAT4 gene

exon / Coding exon / intron
coords / Length(bp) / coords / Length(bp) / coords / Length(bp)
Unkown / Unkown / Unkown / Unkown
1-129 / 129 / 2-129 / 128 / 130-1571 / 1442
1572-1716 / 145 / 1572-1716 / 145 / 1717-57298 / 55582
57299-57397 / 99 / 57299-57397 / 99 / 57398-59764 / 2367
59765-59857 / 93 / 59765-59857 / 93 / 59858-61015 / 1158
61016-61094 / 79 / 61016-61094 / 79 / 61095-63043 / 1949
63044-63129 / 86 / 63044-63129 / 86 / 63130-65142 / 2013
65143-65294 / 152 / 65143-65294 / 152 / 65295-6768 / 2374
67669-67827 / 159 / 67669-67827 / 159 / 67828-68527 / 700
68528-68620 / 93 / 68528-68620 / 93 / 68621-72109 / 3489
72110-72169 / 60 / 72110-72169 / 60 / 72170-72693 / 524
72694-72711 / 18 / 72694-72711 / 18 / 72712-72824 / 113
72825-72918 / 94 / 72825-72918 / 94 / 72919-75013 / 2095
75014-75058 / 45 / 75014-75058 / 45 / 75059-85815 / 10757
85816-85899 / 84 / 85816-85899 / 84 / 85900-87807 / 1908
87808-87906 / 99 / 87808-87906 / 99 / 87907-90316 / 2410
90317-90452 / 136 / 90317-90452 / 136 / 90453-92331 / 1879
92332-92381 / 50 / 92332-92381 / 50 / 92382-92626 / 245
92627-92721 / 95 / 92627-92721 / 95 / 92722-94323 / 1602
94324-94460 / 137 / 94324-94460 / 137 / 94461-94772 / 312
94773-94964 / 192 / 94773-94964 / 192 / 94965-96653 / 1689
96654-96720 / 67 / 96654-96720 / 67 / 96721-97849 / 1129
97850-97958 / 109 / 97850-97958 / 109 / 97959-98991 / 1033
98992-99274 / 283 / 98992-99018 / 27

Note: only can be sure from the exon containing start cordon to the last exon containing stop cordon.

Table S3 The exon/intron organizations of porcine STAT6 gene

exon / Coding exon / intron
coords / Length(bp) / coords / Length(bp) / coords / Length(bp)
1-248 / 248 / 249-3701 / 3453
3702-3838 / 137 / 3723-3838 / 116 / 3839-4252 / 414
4253-4391 / 139 / 4253-4391 / 139 / 4392-4691 / 300
4692-4775 / 84 / 4692-4775 / 84 / 4776-5631 / 856
5632-5770 / 139 / 5632-5770 / 139 / 5771-5895 / 125
5896-5948 / 53 / 5896-5948 / 53 / 5949-6121 / 173
6122-6270 / 149 / 6122-6270 / 149 / 6271-7069 / 799
7070-7201 / 132 / 7070-7201 / 132 / 7202-7315 / 114
7316-7504 / 189 / 7316-7504 / 189 / 7505-8115 / 611
8116-8203 / 88 / 8116-8203 / 88 / 8204-8320 / 117
8321-8443 / 123 / 8321-8443 / 123 / 84444-9036 / 593
9037-9129 / 93 / 9037-9129 / 93 / 9130-9434 / 305
9435-9641 / 207 / 9435-9641 / 207 / 9642-10828 / 1187
10829-10923 / 95 / 10829-10923 / 95 / 10924-11013 / 90
11014-11150 / 137 / 11014-11150 / 137 / 11151-11493 / 343
11494-11640 / 147 / 11494-11640 / 147 / 11641-11848 / 208
11849-11912 / 64 / 11849-11912 / 64 / 11913-12022 / 110
12023-12133 / 111 / 12023-12133 / 111 / 12134-12326 / 193
12327-12419 / 93 / 12327-12419 / 93 / 12420-13454 / 1035
13455-13520 / 66 / 13455-13520 / 66 / 13521-13625 / 105
13626-13754 / 129 / 13626-13754 / 129 / 13755-13842 / 88
13843-15213 / 1371 / 13843-14032 / 190