Supplementary Material s81
SUPPLEMENTARY MATERIAL
Microhabitat segregation and fine ecomorphological dissimilarity between two closely phylogenetically related grazer fishes in an Atlantic Forest stream, Brazil
Environmental Biology of Fishes
Rafael Pereira Leitão, Jorge Ivan Sánchez-Botero, Daniele Kasper, Victor Trivério-Cardoso, Carolina Morais Araújo, Jansen Zuanon & Érica Pellegrini Caramaschi
Corresponding author
Rafael P. Leitão
Laboratório de Ecologia de Peixes – Universidade Federal do Rio de Janeiro
E-mail:
Online Resource Table 1 Environmental characteristics measured in Forested and Deforested sites of Ouro Stream, Southeastern Brazil
Environmentalvariable / Forested / Deforested
Mean / Range / Mean / Range
Altitude (m) / 40 / − / 25 / −
Width (m) / 6.9 / 3.0−9.9 / 7.4 / 3.5−11.7
Depth (cm) / 23.6 / 2.0−99.0 / 25.9 / 2.0−66.0
Current velocity (cm/s) / 32.9 / 0.0−82.1 / 38.0 / 0.0−90.2
Canopy cover (%) / 75.2 / 60.2−94.7 / 0.0 / −
Water temperature (oC) / 22.1 / 18.3−25.6 / 22.5 / 18.1−25.7
Dissolved Oxygen (mg/L) / 7.9 / 7.2−8.4 / 7.6 / 7.3−8.1
Conductivity (µS/cm) / 40.8 / 37.1−44.5 / 45.2 / 42.9−47.5
pH / 7.5 / 6.9−7.6 / 7.2 / 7−7.5
Sand (%) / 57.1 / − / 55.6 / −
Silt/Clay (%) / 7.4 / − / 24.1 / −
Boulder/Rubble/Gravel (%) / 21.8 / − / 6.9 / −
Wood debris/Decaying leaves (%) / 13.0 / − / 2.7 / −
Grassy vegetation (%) / 0.8 / − / 10.7 / −
Online Resource Table 2 Loadings of Linear Discriminant Analysis on microhabitat variables used by Hisonotus notatus and Parotocinclus maculicauda in Forested and Deforested sites of Ouro Stream, Southeastern Brazil
Microhabitat variable / Code / Forested / DeforestedDistance from nearest bank / DNB / 0.64 / 0.64
Water depth / WTD / 0.01 / 0.45
Focal elevation / FEL / -0.15 / 0.81
Average current velocity / ACV / 0.45 / 0.44
Focal-point velocity / FPV / 0.69 / 0.53
Distance from current shelter / DCS / 0.39 / 0.64
Surface-water turbulence / SWT / 0.63 / 0.46
Submerged cover / SCO / -0.02 / 0.22
Above-water cover / ACO / 0.02 / 0.41
Boulder / BOU / 0.35 / −
Rubble-gravel / RGR / 0.14 / -0.21
Wood debris / WOO / -0.17 / 0.70
Decaying leaves / LEA / -0.23 / -0.13
Grassy vegetation / GRA / − / -0.42
Online Resource Table 3 Loadings of Principal Component Analysis (PC1 and PC2), mean and range values of ecomorphological traits measured on 30 individuals of Hisonotus notatus and Parotocinclus maculicauda
Ecomorphological attribute / Code / PC1 / PC2 / H. notatus / P. maculicaudaMean / Range / Mean / Range
Body-flatness index / BoF / 0.83 / -0.21 / 0.75 / 0.68−0.83 / 0.62 / 0.56−0.68
Body depth (mm) / BoD / 0.70 / -0.41 / 5.64 / 4.36−7.42 / 4.72 / 4.01−5.64
Trunk-shape index / TrI / 0.49 / -0.62 / 15.51 / 13.03−18.88 / 14.37 / 12.86−16.81
Ventral-flatness index / VeF / -0.40 / -0.27 / 0.32 / 0.25−0.41 / 0.34 / 0.27−0.39
Peduncle length (mm) / PdL / -0.19 / 0.54 / 10.01 / 8.29−12.67 / 9.60 / 8.39−11.02
Peduncle-flatness index / PdF / 0.53 / -0.37 / 1.39 / 1.27−1.56 / 1.29 / 1.18−1.50
Oral-disc area (mm2) / OdA / -0.87 / -0.10 / 16.05 / 10.63−24.58 / 21.86 / 17.44−25.72
Pectoral-fin length (mm) / PtL / 0.69 / -0.37 / 9.82 / 7.71−12.42 / 8.50 / 7.48−10.08
Pectoral-fin area (mm2) / PtA / -0.49 / -0.44 / 23.75 / 12.84−36.56 / 22.10 / 15.74−34.64
Pectoral-ray thickness (mm) / PtT / -0.38 / -0.57 / 0.84 / 0.62−1.09 / 0.81 / 0.66−1.03
Pelvic-fin length (mm) / PvL / -0.73 / -0.32 / 6.29 / 4.98−7.70 / 6.85 / 6.01−8.11
Pelvic-fin area (mm2) / PvA / -0.77 / -0.39 / 12.64 / 6.66−17.64 / 16.27 / 10.27−24.18
Pelvic-ray thickness (mm) / PvT / -0.83 / -0.17 / 0.55 / 0.41−0.79 / 0.66 / 0.57−0.81
Caudal-fork depth (mm) / CdF / -0.82 / -0.03 / 2.57 / 1.48−3.41 / 3.82 / 2.72−5.45
Online Resource Fig. 1 Schema of individual microhabitat assessment related to submerged cover (any submerged structure located above focal fish) – SCO; and above-water cover (any structure located up to 50 cm from the water surface above focal fish) – ACO. The cover densities were visually estimated using a square (25 x 25 cm) and categorized as: absent (0%), low (1-25%), medium (26-75%), and high (>75%)
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