OIE Reference Laboratory Reports Activities in 2012
Name of disease (or topic) for which you Enzootic Bovine Leukosis are a designated OIE Reference Laboratory:
Address of laboratory National Veterinary Research Institute
Al. Partyzantow 57
24-100 Pulawy, Poland
Tel.: (+48-818893114)
Fax: (+48-818862595)
e-mail address: [email protected]
website: www.piwet.pulawy.pl
Name (including Title) of Head of Dr Krzysztof Niemczuk, Director General of NVRI Laboratory (Responsible Official):
Name (including Title and Position) of OIE Jacek Kuźmak, DVM, PhD, Professor Reference Expert:
Date of submission to the OIE December 25, 2013
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site: (http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards
Test recommended by the OIE Total number of test performed last year
Annual reports of OIE Reference Centres, 2012 1 OIE RL for « Enzootic Bovine Leukosis » – « Jacek Kuźmak » – « Poland »
Indirect diagnostic tests Nationally Internationally
ELISA test (competition, bi-indirect,screening) 3252 60
AGID 256 60
Direct diagnostic tests Nationally Internationally
nPCR and real-time PCR gene env and pol 35 20
ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?
Yes x No
3. Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes x No
4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?
x Yes No
5. Did your laboratory produce vaccines?
Yes x No
6. Did your laboratory supply vaccines to OIE Member Countries?
Yes x No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?
x Yes No
8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
Yes x No
2 Annual reports of OIE Reference Centres, 2012 OIE RL for « Enzootic Bovine Leukosis » – « Jacek Kuźmak » – « Poland »
Name of the new test or diagnostic Description and References (Publication, website, etc.) method or vaccine developed
Real -tme PCR Marzena Rola-Łuszczaka, Christopher Finnegan, Monika Olecha, Bhudipa Choudhury, Jacek Kuźmak (2012) Development of TaqMan real time PCR for the detection of bovine leukemia provirus and its use for clarification of inconclusive serological test results. J Virol Methods in final evaluation
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
9. Did your laboratory carry out diagnostic testing for other OIE Member Countries?
x Yes No
Name of OIE Date No. samples received for No. samples received for Member Country (dd/mm) provision of diagnostic support provision of confirmatory seeking assistance (i.e. from surveillance campaign) diagnoses
Republic of Moldova 5 November 0 40 serum samples 20 samples of leukocytes
Belarus 6 November 0 1 sample developed as national substandard to E05 positive standard OIE serum
10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
x Yes No
Name of the OIE Member Country How the advice was Purpose receiving a technical consultancy provided
Republican Center of Veterinary Interpretation of serological results of In loco Diagnostic Laboratory, Chisinau, ELISA and development of nested PCR Moldova for detection of proviral DNA of BLV
Belaryssian National Veterinary Testing of Belarussion national In loco and remote Center, Minsk, Belarus substandard of BLV positive serum assistance
Institute of Virology, INTA Development of strong positive and Remote assistance Hurlingham, Argentina weak positive and a negative control serum
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
Annual reports of OIE Reference Laboratories, 2012 3 OIE RL for « Enzootic Bovine Leukosis » – « Jacek Kuźmak » – « Poland »
11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?
x Yes No
OIE Member Partners Countries involved Title of the study Duration Purpose of the study (Institutions) other than your country
Molecular 2 years 2011- Aattempt to Urals State Scientific Russia, Ukraine, analysis of BLV 2012 characterise and to Research Institute of Belarus isolates – a link to perform phylogenetic Veterinary Medicine, epidemiological analysis of env gene Ekaterinburg, Russia study sequences from 44 All Russian Research BLV isolates from Institute of Animal different Breeding, Moscov, geographical regions Russia, National of Poland, Belarus, Belarussian Ukraine and Russia. Veterinary Center, Minsk, Belarus, National Scientific Center Institute of Experimental and Clinical Veterinary Medicine, Kharkov, Ukraine
Strengthening of 1 year Comparison of National Veterinary Moldova Veterinary serological and Research Institute, Laboratories in molecular methods Pulawy, Poland Food Chain and for detection of BLV Republican Center of Animal Infectious infection in cattle Veterinary Diseases Control from Moldova Diagnostic in Republic of Laboratory, Chisinau, Moldova Moldova
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
12. Did your Laboratory collect epizootiological data relevant to international disease control?
Yes x No
13. Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes xNo
14. What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals:...... 0 b) International conferences:...... 1
4 Annual reports of OIE Reference Centres, 2012 OIE RL for « Enzootic Bovine Leukosis » – « Jacek Kuźmak » – « Poland »
c) National conferences:...... 2 d) Other:...... /email
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?
x Yes No
a) Technical visits:...... b) Seminars:...... c) Hands-on training courses:...... 2 d) Internships (>1 month):......
Type of technical training Country of origin of the expert(s) No. participants from the provided (a, b, c or d) provided with training corresponding country
c Moldova 1
c Belarus 3
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
16. Does your laboratory have a Quality Management System certified according to an International Standard?
x Yes No
Quality management system adopted
ISO 17025
17. Is your laboratory accredited by an international accreditation body?
x Yes No
Test for which your laboratory is accredited Accreditation body ELISA PCA (Polish Accreditation Center which is a part of ILAC. The accreditation certificate has a no AB1090.
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
x Yes No
ToR: To organise and participate in scientific meetings on behalf of the OIE
Annual reports of OIE Reference Laboratories, 2012 5 OIE RL for « Enzootic Bovine Leukosis » – « Jacek Kuźmak » – « Poland »
19. Did your laboratory organise scientific meetings on behalf of the OIE?
Yes x No
20. Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes x No
ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
21. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?
x Yes No
22. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?
Yes x No
23. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?
x Yes No
Name(s) of relevant OIE Title of the project or contact Scope Reference Laboratories Phylogenetic analysis of BLV Sequence analysis and phylogenetic OIE RL for EBL AHVLA, isolates from East Europe and study of field isolates of BLV from Russia, Weybridge, UK Syberia Ukraine, Poland and Belarus
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.
24. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?
x Yes No
No. participating Participating OIE Member Purpose for inter-laboratory test comparisons1 laboratories Countries Harmonising existing ELISA tests (specify and Belarus, Lithuania, Latvia, Slovakia, 7 sensitivity) Turkey, Ukraine, Marocco
ToR: To place expert consultants at the disposal of the OIE
25. Did your laboratory place expert consultants at the disposal of the OIE?
1
6 Annual reports of OIE Reference Centres, 2012 OIE RL for « Enzootic Bovine Leukosis » – « Jacek Kuźmak » – « Poland »
Yes x No
Annual reports of OIE Reference Laboratories, 2012 7