Lab - Week One: the Scientific Method
Week Two: Enzymes Unit 4 pg 79 (Unit 2 pg 33)
Procedures and worksheet (10 pts):
Each concept illustrated in sections 4.1 and 4.2 in this lab builds on the preceding concepts, so your team should complete these sections in the order in which they are presented.
Do not skip around or multi-task to try and save time – enzymes can be very unforgiving!
Complete 4.1 in your lab book
Be sure you understand the answers on pp. 82 for the quiz!
Complete the following data table about your 4.1. Experiment (based on table 4.1 pg 82)
Tube / Color at end of Experiment / Interpretation1
2
3
What was not present in each of the following tubes :
1.
2.
3.
Complete 4.2 in your lab book
Be sure you understand the answers on pp.85 for the quiz!
Complete the following data table about your 4.2 Experiment (based on table 4.3 pg 84)
Tube / Color at end of Experiment / Interpretation1
2
3
How did tube #1 and #2 differ?
How did tube #1 and #3 differ?
What kind of inhibitor was PTU? How do you know this ?
Skip 4.3A, go to 4.3B using the following setup
Window Side of Room Completes 4.3B (Wall needs to understand and know window side’s results)
Make sure you are following Procedures on p. 90-91 using 1% amylase.
Be extremely careful with pipettes - what would happen if you double-dipped into pH 5 and then 7 without rinsing/changing pipettes?
Begin by analyzing enzyme treated with pH 7 - if this shows no activity, there is a serious reagent or equipment problem and you need to start over.
If pH 7 worked, proceed through 6.5, 7.5, 8, 5.5, and 5 in that order - one at a time to avoid double-dipping and mixing things up.
Wall Side of the Room Completes 4.3C (Window needs to understand and know wall side’s results)
Make sure you are followingProcedures on p. 94-95 using 1% amylase.
Make sure you leave all tubes at the correct temperature while treating AND testing.
Begin by analyzing enzyme at room temperature - if this shows no activity, there is a serious reagent or equipment problem and you need to start over.
Fill in the appropriate tables below for your group.
Complete the following data table for your 4.3B Experiment
Tube / pH Tested: / Time of Starch Disappearance (seconds) / Tube / pH Tested / Time of Starch Disappearance (seconds)1 / 5
2 / 6
3 / 7
4
Prepare a labeled graph that best illustrates your 4.3B data and hypothesis.
Biology 211 Lab Name ______Lab time/day ______
Worksheet- 5points
Complete the following data table for your 4.3C Experiment
Tube / Temp Tested: / Time of Starch Disappearance (seconds) / Tube / Temp Tested / Time of Starch Disappearance (seconds)1 / 5
2 / 6
3 / 7
4
Pepare a labeled graph that best illustrates your 4.3C data and hypothesis.