Table 3. Spider Core Ortholog Annotations. BLASTP (top hit, evalue 1E-10) of Spider Core OGs found in reference taxon (Acanthoscurria geniculata) against either 1) custom BLAST database of Stegodyphus mimosarum proteins downloaded from GenBank (BioProject Accession PRJNA222714; 27,135 protein sequences) or 2) nr, the non-redundant protein database maintained by NCBI. Arthropod Core OG 1 matches to Spider Core OG listed in first column. Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 356 Protein spinster par- custom tial NA 358 MAP/microtubule custom affinity-regulating kinase 3 partial NA 359 Metastasis- custom associated protein MTA1 partial NA 360 Oxysterol-binding custom protein-related protein 9 partial NA 363 NAD-dependent custom deacetylase sirtuin-1 partial NA 365 putative fatty acyl- custom CoA reductase partial 90433 369 Superkiller viralicidic custom activity 2-like 2 par- tial NA 370 hypothetical protein custom X975 26308 partial NA 371 5’-3’ exoribonuclease custom 2 partial 90338 372 Calcium homeostasis custom endoplasmic reticu- lum protein partial NA 375 Lysine-specific custom demethylase 5A partial NA 382 MAGUK p55 sub- custom family member 5 par- tial NA 383 Catenin alpha partial custom NA 388 Protein alan shepard custom partial NA 389 Caskin-1 partial custom NA 392 Glycerophosphocholine custom phosphodiesterase GPCPD1 partial NA 393 Ataxin-2 partial custom 1/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 395 Transcription elonga- custom tion factor SPT6 par- tial NA 399 E3 ubiquitin-protein custom ligase UBR2 partial NA 410 Phosphatidylinositol- custom binding clathrin assembly protein LAP partial NA 411 Sushi von Wille- custom brand factor type A EGF and pentraxin domain-containing protein 1 partial NA 413 Cubilin partial custom NA 415 Ras GTPase- custom activating protein 1 partial NA 416 Cytoplasmic dynein 1 custom intermediate chain 2 partial 89661 418 Bifunctional 3’- custom phosphoadenosine 5’-phosphosulfate synthase partial NA 419 Glycogen synthase custom kinase-3 beta partial NA 422 Irregular chiasm C- custom roughest protein par- tial 89807 423 Actin-interacting pro- custom tein 1 partial NA 425 hypothetical protein custom X975 25889 partial NA 426 Transforming growth custom factor-beta-induced protein ig-h3 partial NA 427 Coatomer subunit al- custom pha partial NA 429 putative E3 ubiquitin- custom protein ligase MY- CBP2 partial NA 430 Macoilin-1 partial custom 90507 431 Stress-activated pro- custom tein kinase JNK par- tial NA 432 5’-3’ exoribonuclease custom 1 partial 2/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 437 U5 small nuclear ri- custom bonucleoprotein heli- case partial 90171 438 U5 small nuclear ri- custom bonucleoprotein com- ponent partial 90478 439 Nucleobindin-1 par- custom tial NA 440 Protein transport pro- custom tein Sec24A partial NA 441 hypothetical protein custom X975 00738 partial NA 443 Receptor-type custom tyrosine-protein phosphatase mu partial 90468 444 Beta-galactosidase custom partial NA 446 hypothetical protein custom X975 09242 partial NA 449 Tyrosine-protein ki- custom nase PR2 partial NA 454 Muscle M-line assem- custom bly protein unc-89 partial 90448 455 Endoplasmin partial custom 89844 456 Ubiquitin-like custom modifier-activating enzyme 1 partial 90063 457 hypothetical protein custom X975 10054 partial 90057 460 Glycogen debranch- custom ing enzyme partial NA 462 Protein LSM14-like custom protein partial 89946 464 Integrin alpha-9 par- custom tial NA 465 Lysine-specific custom demethylase 2B partial 90251 466 Ubiquitin-like custom modifier-activating enzyme ATG7 partial NA 470 Glycerol-3- custom phosphate acyl- transferase 1 mito- chondrial partial 3/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 473 Serine/threonine- custom protein phosphatase 6 regulatory subunit 3 partial NA 474 KAT8 regulatory custom NSL complex subunit 1 partial 89714 475 UDP- custom glucose:glycoprotein glucosyltransferase 1 partial 90034 476 Sterol regulatory custom element-binding protein 1 partial NA 477 Glypican-6 partial custom 90536 478 Pyruvate kinase mus- custom cle isozyme partial NA 480 Influenza virus custom NS1A-binding protein-like protein partial 90317 481 Armadillo segment custom polarity protein partial NA 482 ATP-dependent RNA custom helicase DDX1 par- tial 90596 484 Leucine–tRNA ligase custom cytoplasmic partial 89750 485 Eukaryotic peptide custom chain release factor GTP-binding subunit ERF3A partial 90499 486 Serrate RNA effector custom molecule-like protein partial 89876 491 Exportin-1 partial custom NA 504 Oxysterol-binding custom protein 2 partial NA 505 Serine/threonine- custom protein kinase tousled-like 1 partial 89918 509 Splicing factor 3A custom subunit 1 partial NA 510 Band 4.1-like protein custom 4A partial 90393 512 FACT complex sub- custom unit spt16 partial 4/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 513 ATP-citrate synthase custom partial NA 514 Choline/ethanolaminephosphotransferasecustom 1 partial 89829 518 Ubiquitin carboxyl- custom terminal hydrolase 7 partial NA 519 Carnitine O- custom palmitoyltransferase 1 liver isoform partial 90354 520 Pre-mRNA- custom processing factor 40-like protein partial NA 522 CAP-Gly domain- custom containing linker protein 1 partial NA 524 Carboxypeptidase D custom partial NA 525 hypothetical protein custom X975 17021 partial NA 529 Eukaryotic initiation custom factor 4A-III partial 89912 534 AP-2 complex sub- custom unit alpha-2 partial NA 535 hypothetical protein custom X975 00501 partial NA 537 hypothetical protein custom X975 01393 partial NA 539 Phytanoyl-CoA custom dioxygenase peroxi- somal partial NA 540 Cullin-1 partial custom NA 541 Lethal(3)malignant custom brain tumor-like protein 3 partial 89995 542 putative ATP- custom dependent RNA helicase DDX23 partial NA 544 Ataxin-2 partial custom NA 545 hypothetical protein custom X975 18913 partial NA 549 Kynurenine– custom oxoglutarate transam- inase 3 partial NA 550 Poly(U)-binding- custom splicing factor half pint partial 5/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database 89734 551 putative custom phospholipid- transporting ATPase IIB partial NA 553 hypothetical protein custom X975 21588 partial NA 554 Zinc finger X-linked custom protein ZXDB partial NA 560 Bone morphogenetic custom protein receptor type- 2 partial 90335 561 Threonine–tRNA lig- custom ase cytoplasmic par- tial 90055 562 Valine–tRNA ligase custom partial NA 563 AP-3 complex sub- custom unit delta-1 partial 90418 565 cAMP-dependent custom protein kinase reg- ulatory subunit partial NA 567 Inactive dipeptidyl custom peptidase 10 partial NA 568 Uridine-cytidine custom kinase-like 1 partial NA 569 hypothetical protein custom X975 14140 partial NA 570 Protein transport pro- custom tein Sec23A partial NA 572 Integrator complex custom subunit 4 partial NA 574 putative ATP- custom dependent RNA helicase TDRD9 partial NA 576 Ubiquitin carboxyl- custom terminal hydrolase 47 partial NA 577 hypothetical protein custom X975 19997 partial NA 582 Vitellogenin-6 partial custom NA 584 Protein phosphatase custom 1B partial NA 585 Protein FAM134C custom partial NA 586 YTH domain family custom protein 1 partial 6/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 587 Ral GTPase- custom activating protein subunit beta partial NA 588 Vacuolar protein custom sorting-associated protein 35 partial NA 590 Ubiquitin custom thioesterase Zranb1 partial NA 592 hypothetical protein custom X975 10048 partial NA 595 Angiotensin- custom converting enzyme partial 89663 597 Procollagen-lysine2- custom oxoglutarate 5- dioxygenase 3 partial NA 598 Tropomodulin partial custom NA 600 Ectonucleoside custom triphosphate diphos- phohydrolase 1 partial 89883 601 Monocarboxylate custom transporter 14 partial 90447 602 Aconitate hydratase custom mitochondrial partial NA 603 hypothetical protein custom X975 15148 partial NA 604 Acetyl-coenzyme A custom synthetase cytoplas- mic partial NA 605 ATP-sensitive inward custom rectifier potassium channel 12 partial NA 609 Homeobox protein custom cut partial NA 610 Pumilio-like protein custom partial NA 613 Protein Hook-like custom protein partial NA 615 Trithorax group pro- custom tein osa partial 90516 619 Glutamine synthetase custom 2 cytoplasmic partial 90333 620 Non-specific lipid- custom transfer protein partial 7/232 Supplemental Table 3 – continued from previous page Arthropod Core ID Spider Core ID Putative Ortholog Description Target Database NA 621 E3 ubiquitin-protein custom ligase HUWE1 par- tial NA 622 Stathmin-1-A partial custom NA 623 Neuropathy target es- custom terase partial 90470 624 Serine/threonine- custom protein kinase TAO1 partial 89658 625 Nuclease domain- custom containing protein 1 partial NA 627 Cytoplasmic custom polyadenylation element-binding protein 1-B partial NA 631 Vacuolar protein custom sorting-associated protein 41-like protein partial NA 632 E3 ubiquitin-protein custom ligase RNF123 par- tial NA 633 Pyruvate dehydroge- custom nase phosphatase reg- ulatory subunit mito- chondrial partial NA 635 hypothetical