8th Regional Biophysics Conference Book of Abstracts Zreče, Slovenia 16th to 20th May 2018 Organized by: #ReBiCon2018 Editors: Hana Majaron, Petra Čotar, Monika Koren, Neža Golmajer Zima Published by: Slovenian Biophysical Society Contents 1 Maps 3 2 Programme 5 3 Abstracts 15 4 Organizing and scientific committe 165 1 Maps Poster and exhibition halls: 2 Programme Wednesday, 16. 5. 2018 11:15 Registration (11:15 - 12:45) 14:00 Registration (14:00 - 19:00) 15:45 Opening System biology, bioinformatics, omics 16:00 Plenary lecture: Mario Cindrić (HR) - Identification of microorganisms by mass spectrometry 16:45 Invited lecture: Marko Djordjević (RS) - Biophysical modeling of bacterial immune system regulation 17:15 Oral: Gergely J. Szöllősi (HU) - Tissue size regulation amplifies the effect of asymmetrical cell divisions on cancer incidence Ultrafast phenomena 17:30 Plenary lecture: Janos Hajdu (HU) - X-ray Diffraction from Single Particles and Biomolecules 18:15 Contributing lecture: Petar H. Lambrev (HU) - Ultrafast energy transfer dynamics in photosynthetic light-harvesting complexes probed by two-dimensional electronic spectroscopy 18:45 Oral: Sofia M. Kapetanaki (UK) - Understanding the interplay of heme and carbon monoxide in ion channel regulation 19:15 Get-together dinner Thursday, 17. 5. 2018 8:00 Breakfast & Registration Advanced imaging and spectroscopies 8:45 Plenary lecture: Michal Cagalinec (SK) - Cell biophysics of fluorescent probes for super-resolution optical microscopy 9:30 Invited lecture: Iztok Urbančič (SI) - Advanced STED microscopy of the membrane organisation in activating T-cells 10:00 Contributing lecture: Mario Brameshuber (AT) - Monovalent T-cell antigen receptor complexes drive T-cell antigen recognition 10:30 Oral: Josef Lazar (CZ) - 2P or not 2P: single-photon vs. two-photon polarization fluorescence microscopy 10:45 Break Advanced imaging and spectroscopies 11:15 Contributing lecture: Jurij Sablić (SI) - Open Boundary Molecular Dynamics of DNA Biomedical applications and cancer 11:45 Invited lecture: Imre Derényi (HU) - Cancer Risk and the Somatic Cell-lineage Tree 12:15 Invited lecture: Gregor Bano (SK)- Phosphorescence kinetics of singlet oxygen produced by pulsed laser excitation - selected special cases 12:45 Contributing lecture: Loredana Casalis (IT) - Cell biomechanics as a marker of disease development: the case of calcific aortic valve disease 13:15 Lunch Biomedical applications and cancer 14:45 Contributing lecture: Pavle R. Andjus (RS) - Subcellular astrocytic markers of neuroinflammation in amyotrophic lateral sclerosis as revealed by synchrotron-based spectromicroscopy 15:15 Oral: Irene Jiménez Munguía (RU) - Bilayer lipid membrane as a model of photodynamic therapy processes in cancer cells 15:30 Oral: Michal Nemergut (SK) - Affinity chromatography based on DARPin and maltose binding protein complex formations 15:45 Oral: Attila Gergely Végh (HU) - Membrane tethers reshape intercellular de-adhesion dynamics 16:00 Oral: Janja Mirtič (SI) - Alginate based microcapsules for local probiotic delivery: Evaluation of bacterial entrapment, release, and growth 16:15 Oral: Aleksandra Pavićević (RS) - EPR spin labeling of erythrocytes as a tool for diagnosis and the follow-up of the treatment of Gaucher disease 16:30 Break Computational biophysics 16:45 Invited lecture: Juraj Szavits-Nossan (HR) - mRNA sequence determinants of protein production rate 17:15 Invited lecture: Marko Gosak (SI) - Network science as a gateway to under- standing of emerging dynamics and function of multicellular systems 17:45 Contributing lecture: Magdalena Majekova (SK) - Ligand-based drug design considering specific features of aldose reductase 18:15 Oral: Andrej Dobovišek (SI) - The maximum entropy production principle in enzyme kinetics: novel theoretical insights Poster session with buffet dinner (18:45-22:30) Oral invitations and sponsor presentations at: 19:15 posters from PS-01 to PS-07, 19:45 Fotona, 20:05 posters from PS-76 to PS-90, 20:45 Nanotemper, 21:05 posters from PS-21 to PS-33, 21:45 posters from PS-45 to PS-50. Friday, 18. 5. 2018 8:00 Breakfast & Registration Single molecule and interactions 8:45 Plenary lecture: Gerhard J. Schütz (AT) - Varying label density to probe membrane protein nanoclusters in STORM/PALM 9:30 Contributing lecture: Miklós Kellermayer (HU) - Mechanically-driven ejection of viral DNA 10:00 Oral: Saša Vrhovec Hartman (SI) - Diffusiophoresis of blood cells and vesicles in transient chemical gradients 10:15 Oral: Jakub Nowak (PL) – Cutting edge biophysical tools to determine biomolecular interactions and characterize protein stability in solution 10:30 Oral: Andreas M. Arnold (AT) - Overcoming Blinking Artifacts in Nanocluster Detection with Two Color STORM 10:45 Break Materials & Nanobiophysics 11:15 Invited lecture: Erik Reimhult (AT) - Tailoring biomolecular interactions of core-shell nanoparticles and their application to magnetoresponsive drug delivery vehicles 11:45 Invited lecture: Zuzana Garaiova (SK) - Nanoparticles as drug delivery systems. The interaction with biomembrane models 12:15 Contributing lecture: Jaroslaw Jacak (AT) - Bio-applications for 3D Nano- lithography in Microfluidics 12:45 Contributing lecture: Orestis G. Andriotis (AT) - Poking and pulling collagen: Nanomechanical assessment of native collagen fibrils 13:15 Oral: Hana Majaron (SI) - A relevant in vitro model of the alveolus enables reliable studies of nanomaterial-cell interactions 13:30 Lunch 15:00 Excursions Remote session at Ptuj Castle, »Quo vadis, biophysics« 16:20 Janez Štrancar, opening 16:25 Gerhard Schütz (AT) - Future perspectives of superresolution microscopy 16:30 Mauro Dalla Serra (IT) - COST action ARBRE-MOBIEU 16:35 Andrej Korenić (RS) - AUTOIGG: MSC RISE Action 16:40 Pavle R. Andjus (RS) - Can Belgrade School of Physiology help SpaceX mission to Mars and beyond? 16:45 Laszlo Zimanyi (HU) - ELI-ALPS presentation 16:50 Géza I. Groma (HU) - Ultrafast kinetics of the intramolecular energy migration in NADH 16:55 Tilen Koklič (SI) - In vitro models and H2020 SmartNanoTox 17:00 Jaroslaw Jacak (AT) - Lithography for microfluidics: organ on a chip systems 17:05 András Kincses (HU) - Effects of shear stress on a lab-on-a-chip endothelial model 17:10 General discussion 20:00 Conference dinner Saturday, 19. 5. 2018 8:00 Breakfast & Registration Cell biophysics 8:45 Plenary lecture: Massimo Vassalli (IT) - Mechanosensing in living cells: the emerging role of piezo channels in human patho-physiology 9:30 Invited lecture: Silvia Caponi (IT) - Mechanical and chemical analysis of single living cells investigated by non-contact micro-spectroscopic techniques 10:00 Invited lecture: Nadica Ivošević-de Nardis (HR) - Single cell at the charged interface 10:30 Oral: Edina Szabó-Meleg (HU) - Visualization of transport properties of membrane nanotubes with live cell laser-scanning confocal and super- resolution (SIM) microscopes 10:45 Oral: Miroslav Živić (RS) - ATP dependency of osmotically activated outwardly rectified current in the membrane of cytoplasmic droplets obtained from sporangiophore of model filamentous fungus Phycomyces blakesleeanus 11:00 Break Cell biophysics 11:30 Invited lecture: Gabriella Viero (IT) - Towards understanding of translation biology by coupling positional sequencing and nano-imaging 12:00 Contributing lecture: Matjaž Humar (SI) - Bio-integrated micro-laser particles for sensing, imaging and cell barcoding 12:30 Oral: Peter Galajda (HU) - Fast evolution of antibiotic resistance in microfluidic devices 12:45 Oral: Špela Zemljič Jokhadar (SI) - The role of Arp2/3 complex in cyto- mechanics measured by AFM and optical tweezers 13:00 Lunch Neurobiophysics 14:30 Plenary lecture: Vladana Vukojević (RS) - Functional Fluorescence Microscopy Imaging (fFMI) of fast dynamic processes in live cells by massively parallel Fluorescence Correlation Spectroscopy 15:15 Invited lecture: Johann G. Danzl (AT) - Improving high-resolution optical imaging for (neuro-)biology 15:45 Contributing lecture: Jernej Jorgačevski (SI) - Exocytotic properties of astrocytic vesicles 16:15 Contributing lecture: Jasna Šaponjić (RS) - Altered sleep oscillations as early biomarkers of Parkinson’s disease cholinopathy 16:45 Break Computational biophysics 17:00 Contributing lecture: Péter Horváth (HU) 17:30 Oral: Daniel Grajzel (HU) - Tissue size regulation amplifies the effect of asymmetrical cell divisions on cancer incidence 17:45 Oral: Nebojša Milošević (RS) - Artificial neural networks and multi- dimensional approach in the classification: 2D images of neurons from the human dentate nucleus 18:00 Oral: Alexandra Zahradníková (SK) - Energetics of the open - closed transition in the N-terminal region: importance for the CPVT phenotype Poster session with buffet dinner (18:30-22:30) Oral invitations and sponsor presentations at: 19:00 posters from PS-08 to PS-20, 19:45 Nanion, 20:05 posters from PS-65 to PS-75, 20:45 Fotona, 21:05 posters from PS-34 to PS-44, 21:35 posters from PS-51 to PS-64. Sunday, 20. 5. 2018 8:00 Breakfast Proteins & supramolecular structures 8:45 Plenary lecture: Gregor Anderluh (SI) - Mechanism of membrane interactions of Nep1-like proteins 9:30 Invited lecture: Aleksandar J. Krmpot (RS) - Mapping of hemoglobin residuals in erythrocyte ghosts using two photon excited fluorescence microscopy 10:00 Invited lecture: Beáta Bugyi (HU) – Coordination of actine-microtubule dynamics 10:30 Oral: Parveen Akhtar (HU) - Self-aggregation of light-harvesting complex II in reconstituted membranes mimics non-photochemical quenching