Journal of the Arkansas Academy of Science Volume 72 Article 16 2018 Parasites of the Spotted Sucker, Minytrema melanops (Cypriniformes: Catostomidae) from Arkansas and Oklahoma Chris T. McAllister Eastern Oklahoma St. College, [email protected] Donald G. Cloutman retired, [email protected] Anindo Choudhury St. Norbert College, [email protected] Tomas Scholz Biology Centre of the Czech Academy of Sciences, [email protected] Stanley E. Trauth Arkansas State University, [email protected] See next page for additional authors Follow this and additional works at: https://scholarworks.uark.edu/jaas Part of the Aquaculture and Fisheries Commons, Biology Commons, and the Zoology Commons Recommended Citation McAllister, Chris T.; Cloutman, Donald G.; Choudhury, Anindo; Scholz, Tomas; Trauth, Stanley E.; Fayton, Thomas J.; and Robison, Henry W. (2018) "Parasites of the Spotted Sucker, Minytrema melanops (Cypriniformes: Catostomidae) from Arkansas and Oklahoma," Journal of the Arkansas Academy of Science: Vol. 72 , Article 16. Available at: https://scholarworks.uark.edu/jaas/vol72/iss1/16 This article is available for use under the Creative Commons license: Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0). Users are able to read, download, copy, print, distribute, search, link to the full texts of these articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This Article is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Journal of the Arkansas Academy of Science by an authorized editor of ScholarWorks@UARK. For more information, please contact [email protected], [email protected]. Parasites of the Spotted Sucker, Minytrema melanops (Cypriniformes: Catostomidae) from Arkansas and Oklahoma Cover Page Footnote We thank Drs. Scott L. Gardner and Gabor Racz (HWML) and Renn Tumlison (HSU) for expert curatorial assistance. Appreciation to members of the Oklahoma Department of Wildlife Conservation (ODWC), especially Matt kS oog and Trevor Starks, and Dr. David Neely (Tennessee Aquarium, Chattanooga, TN) and Uland Thomas (Chicago, IL) for assistance with collecting in Oklahoma and Arkansas, respectively. Thanks also to Dr. Michael A. Barger (Peru St. College NE) for identification of the acanthocephalan. The Arkansas Game & Fish Commission and ODWC provided Scientific oC llecting Permits to CTM. Authors Chris T. McAllister, Donald G. Cloutman, Anindo Choudhury, Tomas Scholz, Stanley E. Trauth, Thomas J. Fayton, and Henry W. Robison This article is available in Journal of the Arkansas Academy of Science: https://scholarworks.uark.edu/jaas/vol72/iss1/16 Journal of the Arkansas Academy of Science, Vol. 72 [], Art. 16 Parasites of the Spotted Sucker, Minytrema melanops (Cypriniformes: Catostomidae) from Arkansas and Oklahoma C.T. McAllister1*, D.G. Cloutman2, A. Choudhury3, T. Scholz4, S.E. Trauth5, T.J. Fayton6, and H.W. Robison7 1Science and Mathematics Division, Eastern Oklahoma State College, Idabel, OK 74745 2P. O. Box 197, Burdett, KS 67523 3Division of Natural Sciences, St. Norbert College, 100 Grant Street, DePere, WI 54115 4Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic 5Department of Biological Sciences, Arkansas State University (Emeritus), State University, AR 72467 6Lamar Fish Health Center, U.S. Fish and Wildlife Service, 400 Washington Avenue, Lamar, PA 16848 79717 Wild Mountain Drive, Sherwood, AR 72120 *Correspondence: [email protected] Running Title: Spotted Sucker Parasites Abstract There is a moderate amount of information on parasites of M. melanops from various North American During October 2015, March and April 2016 and localities (Mackiewicz 1968, 1969, Chien and Rogers again between March and April 2017, 15 Spotted Sucker 1970, Bangham 1972, Dechtiar 1972, Combs et al. (Minytrema melanops) were collected from sites in the 1976, 1977, Williams 1976, Christensen et al. 1982, Ouachita (n = 5), Red (n = 1), and St. Francis (n = 5) Williams and Rogers 1984, Hoffman 1999, Dutton and river drainages, Arkansas, and the Arkansas River Barger 2010, Fayton and Kritsky 2013, Gale et al. 2014, drainage, Oklahoma (n = 4), and examined for McAllister et al. 2013, 2015, Oros et al. 2016, 2018). protozoan and metazoan parasites. Found were However, most reports involve fish collected east of the Calyptospora sp., Myxobolus sp., Pseudomurraytrema Mississippi River and nothing is known of specimens alabarrum, Biacetabulum banghami, Penarchigetes from Arkansas. Here we report some new host and oklensis, and Acanthocephalus sp. New host and distributional records for parasites of M. melanops from distributional records are documented for these Arkansas and Oklahoma. parasites. Materials and Methods Introduction During October 2015, March and April 2016 and The Spotted Sucker, Minytrema melanops again between March and April 2017, 15 M. melanops (Rafinesque) is a moderately slim-bodied nearly terete (mean ± 1SD total length [TL] = 179.0 ± 58.6, range 92– sucker that ranges from the Lower Great Lakes basin 243 mm) were collected with a boat electrofisher from (lakes Erie, Huron, and Michigan) and Ontario, Canada, OKLAHOMA: Arkansas River drainage of the Illinois the upper Mississippi Valley south to the Gulf slope River, Cherokee Co. (35° 57' 30.042''N, 94° 52' drainages from Texas to Florida and north on the 10.0272''W) (n = 4); and with seines from Atlantic coast to Cape Fear drainage of North Carolina ARKANSAS: St. Francis River drainage of Crow (Gilbert and Burgess 1980). In Arkansas, M. melanops Creek at Madison, St. Francis Co. (35° 00' 45.4752”N, is widespread and common but nearly absent from the 90° 43' 08.3346”W) (n = 5), Red River drainage of the upper White River drainage (Robison and Buchanan Saline River, Sevier Co. (34° 05' 46.3128"N, 1988). In Oklahoma, the Spotted Sucker is found in 94° 05' 5.0496"W) (n = 1), Ouachita River drainage at about the eastern half of the state (Miller and Robison Calion Lake Spillway, Union Co. (33° 19' 32.2608"N, 2004). It is adapted to slow-moving slightly-turbid 92° 31' 35.1912"W) (n = 3), West Tulip Creek, Dallas waterways of hard-bottomed creeks and small rivers. Co. (34° 01' 13.026"N, 92° 44' 4.1136"W) (n = 1), and They feed on detritus, zooplankton, and various Caddo River, Montgomery Co. (34° 23' 52.7742"N, 93° organisms such as mollusks and other invertebrates 37' 17.6226"W) (n = 1). Specimens were placed in (White and Haag 1977). aerated habitat water and necropsied within 24 hr. We Journal of the Arkansas Academy of Science, Vol. 72, 2018 87 Published by Arkansas Academy of Science, 81 Journal of the Arkansas Academy of Science, Vol. 72 [], Art. 16 C.T. McAllister, D.G. Cloutman, A. Choudhury, T. Scholz, S.E. Trauth, T.J. Fayton, and H.W. Robison followed accepted guidelines for the use of fish in research (Use of Fishes in Research Committee 2014); specimens were overdosed with a concentrated chloretone solution and a mid–ventral incision was made to expose the gastrointestinal (GI) tract and viscera. The GI tract was split longitudinally from esophagus to anus and all internal organs were placed in Petri dishes containing 0.9% saline and examined under a stereomicroscope. A species of monogenean was picked with minuten nadeln directly from the gills of fish (n = 9) previously preserved in 10% formalin then mounted in Gray and Wess medium stained with Gomori’s trichrome. Cestodes from the intestine were fixed in hot tap water without coverslip pressure, transferred to 70–95% (v/v) DNA grade ethanol, stained in Mayer’s carmine, dehydrated in an ethanol series, cleared with eugenol, and mounted in Canada balsam (Scholz et al. 2015). Tissues with encapsulated parasites were fixed in 10% neutral buffered formalin and processed using standard histological methods (Presnell and Schreibman 1997), with sectioning at 8–10 µm followed by staining with hematoxylin and eosin. Voucher specimens of parasites were deposited in the Harold W. Manter Laboratory of Parasitology (HWML), University of Nebraska, Lincoln, Nebraska, or the helminthological collection of the Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic (IPCAS). Host voucher specimens were deposited in the Henderson State University (HSU) fish collection, Arkadelphia, Arkansas. Prevalence, mean intensity ± Figures 1A–B. Calyptospora sp. from Minytrema melanops. (A) 1SD, and range of infection are provided in accordance Low power magnification showing oocysts in encapsulation of liver tissue; scale bar = 250 µm. (B) High power magnification of with terminology given in Bush et al. (1997). encapsulation showing hundreds of oocysts; scale bar = 125 µm. Results and Discussion Type-host and type locality: Gulf Killifish The following is an annotated list of collection and (Fundulus grandis), Ocean Springs, Jackson Co., parasite data as follows: Mississippi (Duszynski et al. 1979). Other reported hosts: Several species of fundulid PROTISTA: APICOMPLEXA: CALYPTOSPORIDAE fishes are known as natural hosts, as well as the Inland Calyptospora sp. (Figs. 1A‒B) Silverside (Menidia beryllina) and Gulf Toadfish Host and locality: 1 M. melanops (213 mm TL) (Opsanus beta) (Solangi and Overstreet 1980, Upton collected on 21 Apr. 2017 from Crow Creek at Madison, and Duszynski 1982, Fournie and Overstreet 1983, St. Francis Co., Arkansas (35° 00' 45.4752”N, 90° 43' 1993, Hawkins et al. 1983, 1984, Oliveira