Novel Insights Novel UITNODIGING voor het bijwonen van de openbare verdediging into Neuronal CAMK2 Function CAMK2 Neuronal into van het proefschrift Novel Insights into Neuronal CAMK2 Function door Martijn J. Kool op dinsdag 11 december 2018 om 13:30 Professor Queridozaal Onderwijscentrum Erasmus MC Dr. Molewaterplein 40 3015 CD Rotterdam MARTIJN KOOL Moutersteeg 11 3024 RG Rotterdam Novel Insights [email protected] into Neuronal PARANIMFEN CAMK2 Function Thomas Hulst Martijn J. Kool [email protected] Laura-anne Grimbergen [email protected] Martijn J. Kool 15932-kool-cover.indd 1 22/10/2018 09:38 NOVEL INSIGHTS INTO NEURONAL CAMK2 FUNCTION Martijn Jacob Kool COLOFON Cover design: Front: "Hippocampus and CAMK2" by Jan Berkelouw Back: "Two Pyramidals" by Greg Dunn Layout: Design Your Thesis, www.designyourthesis.com Printing: Ridderprint B.V., www.ridderprint.nl ISBN: 978-94-6375-178-0 Copyright © 2018 by Martijn Jacob Kool. All rights reserved. Any unauthorized reprint or use of this material is prohibited. No part of this thesis may be reproduced, stored or transmitted in any form or by any means, without written permission of the author or, when appropriate, of the publishers of the publications. NOVEL INSIGHTS INTO NEURONAL CAMK2 FUNCTION NIEUWE INZICHTEN IN DE FUNCTIE VAN NEURONAAL CAMK2 PROEFSCHRIFT ter verkrijging van de graad van doctor aan de Erasmus Universiteit Rotterdam op gezag van de rector magnificus Prof.dr. R.C.M.E. Engels en volgens besluit van het College voor Promoties. De openbare verdediging zal plaatsvinden op 11 december 2018 om 13:30 uur door Martijn Jacob Kool geboren te Rotterdam PROMOTIECOMMISSIE Promotor: Prof.dr. Y. Elgersma Overige leden: Prof.dr. J.G.G. Borst Prof.dr. S.A. Kushner Prof.dr. H.W.H.G. Kessels Copromotor: Dr. G.M. van Woerden TABLE OF CONTENTS Preface 7 Chapter 1. General Introduction 9 Chapter 2. Temporal and region-specific requirements of aCaMKII in 57 spatial and contextual learning Chapter 3. The molecular, temporal and region-specific requirements of 79 the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in mouse locomotion Chapter 4. CAMK2-dependent signaling in neurons is essential for 105 survival Chapter 5. Bidirectional changes in excitability upon loss of both CAMK2A 135 and CAMK2B Chapter 6. General Discussion 161 Appendix. English summary 177 Nederlandse samenvatting 179 List of Publications 183 Curriculum Vitae 185 PhD Portfolio 187 Dankwoord 189 PREFACE This dissertation will focus on the role of the two majorly abundant protein kinases CAMK2A and CAMK2B present in the brain. I have decided to highlight not all but only certain aspects of the literature on CAMK2A and CAMK2B for three reasons. First, CAMK2A has already been profoundly studied considering the vast body of literature on this protein subunit. CAMK2B however, has not been given the same level of attention as its highly homologous protein isoform CAMK2A. As such, a substantial part of the introduction of this dissertation will try to summarize all current knowledge on this relatively unknown protein subunit CAMK2B. Second, this dissertation will try to elucidate the unique and common functions of CAMK2A and CAMK2B, thereby helping in understanding the full spectrum of the CAMK2 protein in neuronal function. Both protein subunits arose from a common ancestral gene, making these two proteins highly homologous. Therefore, for many functions in the brain it is not known which are carried out by CAMK2A, which are carried out by CAMK2B or which are carried out by both. Third, as a result of this high homology, dissociating between these two proteins using pharmacological inhibitors has so far proved impossible. Regardless, CAMK2 inhibitors are widely used to unravel novel functions of CAMK2. Thorough knowledge of these inhibitors is important to distinguish between direct effects of the drugs versus off- target effects. Therefore, the introduction of this dissertation will try to summarize the knowledge on most current drugs available for CAMK2 and assert their specificity. On a different note, the use of the words CAMK2A, CAMK2B and CAMK2 can be confusing, both for outsiders as well as among experts in the field. To clarify, this dissertation will refer to CAMK2 as the entire holoenzyme, either containing both CAMK2A and CAMK2B (heteromeric holoenzyme) as well as only CAMK2A (homomeric holoenzyme). If certain traits can be ascribed to single subunits of the holoenzyme, the specific name of that isoform (e.g. CAMK2A, CAMK2B) will be mentioned. 7 Chapter 1 General Introduction General Introduction “Nothing in biology makes sense except in the light of evolution” Theodosius Dobzhansky 1 1.1 A BILLION-YEAR-OLD GENE Ca2+/Calmodulin-dependent protein kinase II (CAMK2) is a protein that can be transcribed from 4 different genes which are estimated to have evolved from a common ancestral gene over 1 billion years ago (Ryan and Grant, 2009). CAMK2 is thought to have arisen at the start of the kingdom of Metazoa (Ryan and Grant, 2009) and has gradually evolved and acquired a variety of functions in a plethora of species. As a result, the CAMK2 gene can be found throughout the animal kingdom, with only one genetic copy in (among others): sponges (A. Queenslandica (Ryan and Grant, 2009) and Suberitus domuncula (Krasko et al., 1999)), nematodes (C. Elegans; in which CAMK2 is referred to as uncoordinated (unc)-43 (Reiner et al., 1999)) and insects (D. melanogaster (Ohsako et al., 1993)), three copies in (among others): frogs (Xenopus laevis (Stevens et al., 2001)) and 4 copies in (among others): mice (Mus Musculus (Hanley et al., 1989)), chicken (Gallus Gallus (Li et al., 1998)), and humans. The multiple gene copies of CAMK2 found in frogs and higher vertebrates most likely arose from duplication of a common ancestral CAMK2 gene (Tombes et al., 2003). These duplications in turn are thought to have arisen from two whole genome duplication events (paleopolyploidy) that have occurred in a common ancestor of chordates and echinodermata in the deuterostomes clade (McLysaght et al., 2002). Therefore, the different CAMK2 genes can be referred to as paralogs. Today, the CAMK2 protein is mainly known for its neuronal and cardiac functions. Interestingly however, the ancestral CAMK2 protein may have started as a non-neuronal and non-cardiac protein, since species in the phylum porifera (sponges) that have 1 copy have neither nervous nor circulatory system. Therefore, the initial function of CAMK2 in calcium homeostasis might have been much broader than its current specific functions in the heart and the brain. This thesis will specifically focus on CAMK2 functions in the brain. 11 Chapter 1 The four protein isoforms known in higher vertebrates are CAMK2A, CAMK2B, CAMK2G and CAMK2D (A for alpha, B for beta, G for gamma and D for delta), all coming from the 4 paralog genes Camk2a, Camk2b, Camk2g and Camk2d located on different chromosomes (Table 1). Camk2b and Camk2g are the most closely related of these, and it is likely that Camk2a arose later in evolution as it is not present in amphibians (Tombes et al., 2003). Table 1. Overview of CAMK2 proteins and their chromosomal location in mice and humans Protein Genea Mouse Chromosome Human Chromosome CAMK2A Camk2a 18 5 CAMK2B Camk2b 11 7 CAMK2G Camk2g 14 10 CAMK2D Camk2d 3 4 a Based on the nomenclature for mice Taken together, it can safely be assumed that in higher vertebrates the four CAMK2 paralogs have each developed to fulfill unique roles. On the other hand, considering their divergence from a common ancestral gene, an argument can also be made that certain functions present in the ancestral gene have been conserved in two, three or maybe all four paralogs. 1.2 CAMK2 STRUCTURE, DOMAIN ORGANIZATION AND REGULATION CAMK2 is quite an unusual protein, in the sense that it can auto-assemble into a larger protein, called a holoenzyme. This can be done using only proteins transcribed from one gene (homomers), or with a mixture of proteins coming from different CAMK2 genes (heteromers). In this section, the structure of this holoenzyme will be discussed as well as the domain organization and the regulation of a single CAMK2 protein. 1.2.1 CAMK2 Structure As mentioned above, CAMK2 can form a holoenzyme. More specifically, it can form a dodecameric holoenzyme, coming from the Greek dodeca (twelve) and meric (part of), meaning that CAMK2 is a holoenzyme consisting of, on average, twelve protein subunits 12 General Introduction (Colbran and Brown, 2004; Chao et al., 2011). The holoenzyme is made of two rings of 6 subunits each, placed on top of each other in a doughnut-like shape (Rosenberg et al., 2005) (Figure 1). CAMK2A and CAMK2B can form heteromeric oligomers (Shen et al., 1998). The same 1 study mentioned but did not show that CAMK2G and CAMK2D can also form heteromeric holoenzymes with CAMK2A and CAMK2B, although later reports suggested that CAMK2G can move to the nucleus without translocation of any of the other isoforms (Ma et al., 2014). One does not necessarily exclude the other, as CAMK2G can detach from CAMK2A or CAMK2B upon translocation to the nucleus. Exchange of subunits between holoenzymes has been observed as well, but so far only for CAMK2A subunits (Stratton et al., 2013). Nevertheless, it is still not definitively clear whether CAMK2G and CAMK2D can form heteromers with CAMK2A and CAMK2B. 1.2.2 Domain organization and regulation The CAMK2 protein can be divided into multiple functional domains. These domains can be found in all 4 isoforms. Considering yet again that these genes have come from a common ancestral gene, it is perhaps not surprising that all 4 CAMK2 isoforms are highly homologous in their domain organization. In rats for example, it has been found that there is an 89-93% sequence homology between two of these domains (the catalytic and regulatory domain which will be discussed below) (Tobimatsu and Fujisawa, 1989).