UNIVERSITATEA DE ŞTIINŢE AGRICOLE ŞI MEDICINĂ VETERINARĂ A BANATULUI TIMIŞOARA FACULTATEA DE MEDICINĂ VETERINARĂ LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ TIMIŞOARA VOLUMUL LII (3) SCIENTIFICAL PAPERS VETERINARY MEDICINE EDITORIAL BOARD Prof. VIOREL HERMAN, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Prof. ILEANA NICHITA, PhD, DVM – Faculty of Veterinary Medicine BUASVM Timisoara Prof. SORIN MORARIU, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Prof. MARIUS PENTEA, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Lecturer DORU MORAR, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Prof. ION OPRESCU, PhD, DVM – Faculty of Veterinary Medicine BUASVM Timisoara Prof. EMIL TIRZIU, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara EDITOR-IN-CHIEF: Assoc. Prof. NARCISA MEDERLE, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Editorial assistants: Lecturer LILIANA CĂRPINIȘAN, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Lecturer ALINA GHIȘE, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Lecturer ADRIANA MORAR, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Assistant JELENA SAVICI, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Assistant MIHAELA PETCU, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Assoc. Prof. CORINA PASCU, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Lecturer IONICA IANCU, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Assistant IULIA BUCUR, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara SCIENTIFIC ADVISORY COMMITTEE Prof. DUSAN ORLIC, PhD, DVM - Scientific Veterinary Institute Novi Sad, Serbia Prof. JOVAN BOJKOVSKI, PhD, DVM - Faculty of Veterinary Medicine, Belgrade, Serbia Prof. IVAN PAVLOVIĆ, PhD, DVM - Scientific Veterinary Institute, Belgrade, Serbia Prof. MANFRED GAREIS, PhD, DVM - Ludwig-Maximilians-Universität München, Germany Prof. HANS WERNER KRUTSCH, PhD, DVM – Institute of Meet Science, Nurenberg, Germany Prof. NICOLAE MANOLESCU, PhD, DVM, Dr. HC – Oncologic Institute ”Prof. dr. Al. Trestioreanu” Bucharest, Corresponding member of Romanian Academy, Titular member of Romanian Academy of Medical Science, Honorific member of Romanian Academy of Agricultural and Forestry Science Prof. MIHAI DECUN, PhD, DVM – Faculty of Veterinary Medicine BUASVM Timisoara, Titular member of Romanian Academy of Agricultural and Forestry Science Prof. HORIA CERNESCU, PhD, DVM, Dr. HC - Faculty of Veterinary Medicine BUASVM Timisoara, Titular member of Romanian Academy of Agricultural and Forestry Science, Member of BASeVA Prof. GHEORGHE DARABUS, PhD, DVM – Faculty of Veterinary Medicine BUASVM Timisoara, Titular member of Romanian Academy of Agricultural and Forestry Science Prof. IOAN GROZA, PhD, DVM - Faculty of Veterinary Medicine UASVM Cluj Napoca Prof. CORNEL CATOI, PhD, DVM - Faculty of Veterinary Medicine UASVM Cluj Napoca Prof. VASILE COZMA, PhD, DVM - Faculty of V eterinary Medicine UASVM Cluj Napoca Prof. GABRIEL PREDOI, PhD, DVM - Faculty of Veterinary Medicine UASVM Bucuresti Prof. LIVIU MIRON, PhD, DVM - Faculty of Veterinary Medicine UASVM Iasi Prof. GHEORGHE SĂVUTĂ, PhD, DVM - Faculty of Veterinary Medicine UASVM Iasi Prof. ALEXANDRA TRIF, PhD, DVM, Dr. HC. - Faculty of Veterinary Medicine BUASVM Timisoara Prof. NICOLAE CĂTANĂ, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Prof. ROMEO CRISTINA, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Prof. CORNEL IGNA, PhD, DVM - Faculty of Veterinary Medicine BUASVM Timisoara Prof. MIHAI MAREȘ, PhD, DVM - Faculty of Veterinary Medicine UASVM Iasi Assoc. Prof. FLORIN BETEG, PhD, DVM - Faculty of Veterinary Medicine UASVM Cluj Napoca To be cited: LUCRARI STIINTIFICE: MEDICINA VETERINARA TIMISOARA (SCIENTIFICAL PAPERS: VETERINARY MEDICINE TIMISOARA), vol. LII (3), 2019 Available online at: http://www.usab-tm.ro/USAMVBT_Revista_ro_1086.html Indexed and/or abstracted in: CABI Full Text, CAB Abstracts, Ulrich's Periodicals Directory Editor: AGROPRINT TIMISOARA ISSN: 1221-5295 Printed by: IMPRIMERIA MIRTON TIMISOARA UNIVERSITATEA DE ŞTIINŢE AGRICOLE ŞI MEDICINĂ VETERINARĂ A BANATULUI TIMIŞOARA FACULTATEA DE MEDICINĂ VETERINARĂ LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ TIMIŞOARA VOLUMUL LII (3) SCIENTIFICAL PAPERS VETERINARY MEDICINE TIMIŞOARA 2019 LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ VOL. LII(3), 2019, TIMIŞOARA THE USE OF PARASITICIDE TREATMENTS IN CARP LARVICULTURE ATHANASOPOULOS L., JECU E., TENCIU M., MOCANU E., NISTOR V., SIMIONOV I. A., CHIOVEANU M. C. Institute of Research and Development for Aquatic Ecology, Fishing and Aquaculture of Galati 800211, 54 Portului St., Galați, Romania E-mail: [email protected] Summary The present study aimes to document fish pathology on carp larvae and it was conducted over a period of 10 days. The biological material was produced in Brates Experimental Farm, by the use of controlled semi-natural pond breeding technology, during april-may 2018. Wet mount microscopic analysis revealed parasitic diseases. The parasites were counted and reported as predominant species of protozoan ciliate - Trichodina sp. Therefore, the treatment applied on carp larvae was consisted by the administration of bleaching powder - Ca (ClO)2 - in a dose of 10 kilograms per hectare. The treatment had 2 purposes as it follows: prophylactic purpose (0-10 days after hatching) in BR 11 and curative purpose (0-13 days after hatching and 0-16 days after hatching) in BR 12, respectively in BR 11. In order to establish the effectiveness of applied treatments, the degree of fish parasitic infestation was determined. At the end of the experiment period, the highest survival percentage of harvested fish was recorded in the prophylactic treatment option. Keywords: carp larvae, Trichodiniosis, bleaching powder Larviculture is known to be of great global significance and has attained an elevated interest in Romania as well, due to the nutritional optimization within the rearing technology and the use of parasiticide treatments, which play a key role in reducing juvenile fish mortality. Ectoparasitic protozoa manifest variable effects on their hosts. Pathological effects are dependent on parasite population density and the fish physiological condition. Stressful conditions compromise the fish capacity to counteract infection. Heavy infections by ectoparasitic protozoars are mainly found in fish specimen aged less than one year old, reared in overcrowded habitats with poor water quality and under stressful conditions. In these circumstances, opportunistic and ubiquitous parasites species are found. However, infections in adult fish speimen are rare and host-specific species predominate. Heavy infections with ubiquitous trichodinids occur in carp larvae and fry in hatcheries and nursery ponds, and likewise as fish grow, they are replaced by more specialised species. After the controlled semi-natural pond breeding process of carp (Cyprinus carpio Linnaeus, 1758), complications may occur. Decreased functionality of the imunitary system of carp fry may happen after the exogenous feeding shift, due to environmental conditions such as water eutrophication. This fact can cause frequent parasitic disease. Available treatment substances, according to the 5 LUCRĂRI ŞTIINŢIFICE MEDICINĂ VETERINARĂ VOL. LII(3), 2019, TIMIŞOARA present European regulation, are limited. Therefore, scientific researches and studies on the identification of new substances to combat fish parasitism are imperative. For this purpose, an experiment was initiated by using lime chloride as the main substance for the treatment and disinfection of water. The substance used in the indicated dose does not present toxicity for fish, neighter remanence in the water or mud. As well, the substance has not been observed to negatively influence the growth rate of the biological material. In Brates Experimental Farm, when the water temperature rises, explosive development of Cyzicus spp., Lepidurus apus and Streptocephalus spp. occurs. Also, invasion with the protozoan parasite Trichodina spp. is manifested. The necesity of this experiment is based on the immediate need of fish farmers to identify new methods of treatment. It is imperative to determine which substances are most suitable considering the cost-benefit concept and the development stage of the biological material (the larval stages). Materials and methods The biological material used consisted of carp larvae, obtained by semi- semi-natural pond breeding technology, in the Brates Experimental Farm of I.C.D.E.A.P.A. Galati. The carp larvae hatched at the end of April 2018. The experiment was carried out for a period of 10 days. The experimental ponds were treated for the prevalent parasite Trichodina spp., with 10 kg of lime chloride (CaOCl2) per hectare (6). Experimental variants: • BR 11 - control pond (absent of parasitic disease) - in which the prophylactic treatment was administered to 10 days old carp larvae, on 08.05.2018; • BR 12 - (accidental presence of the parasite Trichodina spp. on the tegument of the biological material) - in which the curative treatment was administred, of the first sporadic symptoms of parasitic disease, to the 13 days old carp larvae, on 05.11.2018; • BR 13 – (degree of massive infestation of the biological material) - in which curative treatment was applied to the 16 days old carp larvae, on 05.14.2018. The treatment was repeated after two days, on 05.16.2018, by using an identical treatment to the previous one. At the end of