Mycosphere 10(1): 1–186 (2019) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/10/1/1 Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits Jayasiri SC1-3, Hyde KD1,2, Jones EBG4, McKenzie EHC5, Jeewon R6, Phillips AJL7, Bhat DJ8, Wanasinghe DN1,2, Liu JK9, Lu YZ2,10, Kang JC10, Xu J1, Karunarathna SC1 1Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, P.R. China 2Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand 3School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand 4Department of Botany and Microbiology, College of Science, King Saud University, P.O Box 2455 Riyadh 11451, Kingdom of Saudi Arabia 5Manaaki Whenua Landcare Research, Private Bag 92170, Auckland, New Zealand 6Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit, Mauritius 7Universidade de Lisboa, Faculdade de Ciências, Biosystems and Integrative Sciences Institute (BioISI), Campo Grande, 1749-016 Lisbon, Portugal 8Formerly, Department of Botany, Goa University, Goa, India; No. 128/1-J, Azad Housing Society, Curca, P.O. Goa Velha, 403108, India 9School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, P.R. China 10Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, Guizhou Province 550025, P.R. China Jayasiri SC, Hyde KD, Jones EBG, McKenzie EHC, Jeewon R, Phillips AJL, Bhat DJ, Wanasinghe DN, Liu JK, Lu YZ, Kang JC, Xu J, Karunarathna SC 2019 – Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits. Mycosphere 10(1), 1–186, Doi 10.5943/mycosphere/10/1/1 Abstract Dothideomycetes is one of the largest and most diverse class of ascomycetes. Its members are reported from many plant parts, but less has been reported from wild seed pods and fruits. Dothideomycetes can be seed-borne or colonize fruits and seed pods when they fall to the ground. We studied the Dothideomycetes found on wild fruits and seed pods, mainly in Thailand (tropical), and to a lesser extent, in China (temperate) and UK (temperate). We describe eight new genera, 50 new species, provide 38 new host records and propose seven new combinations. The new genera are: Amorocoelophoma (Amorosiaceae), Cylindroaseptospora (Didymosphaeriaceae), Discotubeufia (Tubeufiaceae), Leucaenicola (Bambusicolaceae), Neolindgomyces (Lindgomycetaceae), Pleohelicoon (Pleomonodictydaceae), Quercicola (Astrosphaeriellaceae) and Xenoastrosphaeriella (Astrosphaeriellaceae). The new species are: Acrocalymma pterocarpi, Allophoma siamensis, Amorocoelophoma cassiae, Anteaglonium gordoniae, Atrocalyx krabiensis, Austropleospora keteleeriae, Caryospora quercus, Cladosporium entadae, C. magnoliigena, Cycasicola leucaenae, Cylindroaseptospora leucaenae, C. siamensis, Delitschia nypae, Dictyocheirospora lithocarpi, Didymella magnoliae, Didymocrea leucaenae, Diplodia magnoliensis, Discotubeufia browneae, Dothiorella lampangensis, Ernakulamia krabiensis, Gloniopsis fluctiformis, G. leucaenae, Lasiodiplodia avicenniarum, L. swieteniae, Leucaenicola Submitted 6 January 2019, Accepted 13 February 2019, Published 26 February 2019 Corresponding Author: Karunarathna SC – e-mail – [email protected] 1 aseptata, L. phraeana, Neodeightonia planchoniae, Neolindgomyces pandanae, Neopyrenochaeta cercidis, Neoroussoella entadae, N. leucaenae, Ochroconis ailanthi, Periconia delonicis, Phaeosphaeria sinensis, Pleohelicoon fagi, Psedochaetosphaeronema siamensis, Pseudoberkleasmium acaciae, Pseudocoleophoma bauhiniae, Pseudofusicoccum calophylli, Pseudohelicomyces quercus, Pseudopithomyces entadae, Quercicola fusiformis, Q. guttulospora, Remotididymella bauhiniae, Spegazzinia radermacherae, Stagonosporopsis pini, Stomiopeltis phyllanthi, S. sinensis, Tubeufia entadae and Vaginatispora nypae. These novelties represent both sexual and asexual morphs of species in 35 families of this class. Taxonomic novelties are morphologically illustrated and phylogeny investigated based on multi-gene sequence data. Our results indicate that the plant genus Leucaena harbours higher species diversity. Keywords – 58 new taxa – Amorocoelophoma – ascomycete – bitunicate – Leucaenicola – Neolindgomyces Dothideomycetes sensu O.E. Erikss. & Winka, Myconet 1: 5 (1997) Pleosporomycetidae C.L. Schoch, Spatafora, Crous & Shoemaker, Mycologia 98 (6): 1048 (2007) Gloniales Jayasiri & K.D. Hyde, Mycosphere 9 (4): 809 (2018) Gloniaceae E. Boehm, C.L. Schoch & Spatafora, Mycological Research 113 (4): 468 (2009) 1. Purpurepithecium murisporum Jayasiri & K.D. Hyde Cryptogamie Mycologie 38, 246 (2017) Hysteriales Lindau, Die Natürlichen Pflanzenfamilien nebst ihren Gattungen und wichtigeren Arten 1 (1): 265 (1897) Hysteriaceae Chevall., Flore Générale des Environs de Paris 1: 432 (1826) 2. Gloniopsis fluctiformis Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):15 (2019), new species 3. Gloniopsis leucaenae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):16 (2019), new species 4. Rhytidhysteron rufulum (Spreng.) Speg., Anales de la Sociedad Científica Argentina 90(1-6): 177 (1921) [1920], new host record Pleosporales Luttr. ex M.E. Barr, Prodromus to class Loculoascomycetes: 67 (1987) Acrocalymmaceae P.W. Crous & T. Trakunyingcharoen, IMA Fungus 5 (2): 404 (2014) 5. Acrocalymma pterocarpi Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):20 (2019), new species Amniculicolaceae Y. Zhang ter, C.L. Schoch, J. Fourn., Crous & K.D. Hyde, Studies in Mycology 64: 95 (2009) 6. Vargamyces aquaticus (Dudka) Tóth., Acta Mus. Silesiae, Ser. A 25(3–4): 403. (1980), new host record Amorosiaceae Thambug. & K.D. Hyde, Fungal Diversity 74: 252 (2015) 7. Amorocoelophoma Jayasiri, EBG Jones & K.D. Hyde, Mycosphere 10(1):25 (2019), new genus 8. Amorocoelophoma cassiae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):25 (2019), new species 2 Antigloniaceae K.D. Hyde & A. Mapook, Fungal Diversity 63 (1): 33 (2013) 9. Anteaglonium gordoniae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):28 (2019), new species Astrosphaeriellaceae Phook. & K.D. Hyde, Fungal Diversity 74: 161 (2015) 10. Quercicola Jayasiri, EBG Jones & K.D. Hyde, in Fungal Diversity 10(1):29 (2019), new genus 11. Quercicola fusiformis Jayasiri, EBG Jones & K.D. Hyde, Mycosphere 10(1):30 (2019), new species 12. Quercicola guttulospora Jayasiri, EBG Jones & K.D. Hyde, Mycosphere 10(1):32 (2019), new species 13. Caryospora quercus Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):34 (2019), new species 14. Xenoastrosphaeriella Jayasiri, EBG Jones & K.D. Hyde, Mycosphere 10(1):36 (2019), new genus 15. Xenoastrosphaeriella tornata (D. Hawksw. & Boise) Jayasiri & K.D. Hyde, Mycosphere 10(1):36 (2019), new combination Bambusicolaceae D.Q. Dai & K.D. Hyde, Fungal Diversity 63 (1): 49 (2013) 16. Leucaenicola Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):37 (2019), new genus 17. Leucaenicola aseptata Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):39 (2019), new species 18. Leucaenicola phraeana Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):40 (2019), new species Delitschiaceae M.E. Barr, Mycotaxon 76: 109 (2000) 19. Delitschia nypae Jayasiri, E.B.G. Jones & K.D. Hyde, Fungal Diversity 10(1):41 (2019), new species Dictyosporiaceae Boonmee & K.D. Hyde, Fungal Diversity 80: 462 (2016) 20. Dictyocheirospora lithocarpi Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):45 (2019), new species 21. Dictyocheirospora nabanheensis Tibpromma & K.D. Hyde, Fungal Diversity 92: 10 (2019), new host record 22. Pseudocoleophoma bauhiniae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):47 (2019), new species 23. Pseudodictyosporium wauense Matsush. Bulletin of the National Science Museum Tokyo 14(3): 473 (1971), new host record Didymellaceae Gruyter, Aveskamp & Verkley, Mycological Research 113 (4): 516 (2009) 24. Allophoma siamensis Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):51 (2019), new species 25. Didymella coffeae-arabicae (Aveskamp, Verkley & Gruyter) Qian Chen & L. Cai, Studies in Mycology 82: 175 (2015), new host record 26. Didymella magnoliae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):55 (2019), new species 27. Nothophoma quercina (Syd. & P. Syd.) Qian Chen & L. Cai, Studies in Mycology 82: 213 (2015), new host record 28. Remotididymella bauhiniae Jayasiri, E.B.G. Jones & K.D. Hyde, Fungal Diversity 10(1):58 (2019), new species 3 29. Stagonosporopsis pini Jayasiri, E.B.G. Jones & K.D. Hyde, Fungal Diversity 10(1):61 (2019), new species Didymosphaeriaceae Munk, Dansk botanisk Arkiv 15 (2): 128 (1953) 30. Austropleospora archidendri (Verkley, Göker & Stielow) Ariyaw. & K.D. Hyde, Fungal Diversity 75: 64 (2015), new host record 31. Austropleospora keteleeriae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):65 (2019), new species 32. Cylindroaseptospora Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):67 (2019), new genus 33. Cylindroaseptospora leucaenae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):67 (2019), new species 34. Cylindroaseptospora siamensis Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):68 (2019), new species 35. Didymocrea leucaenae Jayasiri, E.B.G. Jones & K.D. Hyde, Mycosphere 10(1):69 (2019), new species 36. Pseudopithomyces chartarum (Berk. & M.A. Curtis) Jin F. Li, Ariyaw. & K.D. Hyde, Fungal Diversity