Pharmaceutical Applications Notebook Controlled Drugs Table of Contents Index of Analytes .......................................................................................................................................................................4 Introduction to Pharmaceuticals ................................................................................................................................................5 UltiMate 3000 UHPLC+ Systems ............................................................................................................................................6 IC and RFIC Systems ................................................................................................................................................................7 MS Instruments .........................................................................................................................................................................8 Chromeleon 7 Chromatography Data System Software ..........................................................................................................9 Process Analytical Systems and Software ..............................................................................................................................10 Automated Sample Preparation ..............................................................................................................................................11 Analysis of Controlled Drugs ...............................................................................................................................................12 Determination of Bethanechol by Ion Chromatography ........................................................................................................13 Determination of Nevirapine Using HPLC with UV Detection ............................................................................................17 Determination of Carbachol In Ophthalmic Solutions Using a Reagent-Free Ion Chromatography System ......................24 Determination of Verapamil Hydrochloride Purity Using the Acclaim PA Column .............................................................32 Determination of Glucosamine in Dietary Supplements Using HPAE-PAD ........................................................................36 Determination of Urea and Allantoin in Cosmetics Using the Acclaim Mixed-Mode HILIC Column ................................45 Analysis of Sumatriptan in Cerebro-spinal Fluid Using Capillary LC/MS/MS ....................................................................51 Budesonide and Related Substances on Acclaim 300 C18 ....................................................................................................53 Glucocorticosteroids in Serum on Acclaim 120 C18 .............................................................................................................54 Propafenone and Related Substances Using Acclaim PA .......................................................................................................55 Loperamide in Anti-diarrhea Tablets on Acclaim 120 C8. .....................................................................................................56 Simultaneous Analysis of a Basic Drug and Its Counterion (Trimipramine) ........................................................................57 Simultaneous Separation of Bisoprolol and Its Counterion Fumarate ...................................................................................58 Rapid Separation of Paclitaxel and Related Compounds in Palitaxel Injection ....................................................................60 Separation of Etoposide Using an Acclaim Phenyl-1 Column ..............................................................................................63 UHPLC Separation of Nine Corticosteroids in Under Four Minutes ....................................................................................65 Determination of Methacholine Chloride and Potential Impurities Using a Reagent-Free Ion Chromatography System .......................................................................................................69 HPLC Assay Method for Drug Products Containing Anti-Tuberculosis Active Pharmaceutical Ingredients ......................77 Sensitive and Fast Determination of Endothall in Water Samples by IC-MS/MS ................................................................88 Mass Spectrometry Compatible Separation of Itraconazole and Related Substances by UHPLC .......................................94 Determination of Ethyl Sulfate Impurity in Indinavir Sulfate Drug Using Ion Chromatography ......................................100 Evaluation of the USP Risedronate Sodium Assay ..............................................................................................................105 Column Selection Guide .....................................................................................................................................................113 Thermo Scientific Acclaim Column Selection Guide ..........................................................................................................114 Transferring HPLC Methods to UHPLC .........................................................................................................................115 Easy Method Transfer from HPLC to RSLC with the Dionex Method Speed-Up Calculator ...........................................116 Index of Analytes Acetylcholine ...................................................................................69 Inositol .............................................................................................36 Allantoin ..........................................................................................45 Isoniazid ...........................................................................................77 Ammonium ......................................................................................69 Itraconazole .....................................................................................94 Betamethasone .................................................................................65 Lamivudine ......................................................................................17 Bethanechol ...............................................................................13, 24 Loperamide ......................................................................................56 Bisoprolol ........................................................................................58 Lithium ............................................................................................69 Budesonide ......................................................................................53 Magnesium ......................................................................................69 b-Methylcholine ..............................................................................69 Mannitol ...........................................................................................36 Calcium ............................................................................................69 Methacholine ...................................................................................69 Carbachol .........................................................................................24 Methyl prednisolone ........................................................................54 Cations .............................................................................................13 Methylprednisone ............................................................................65 Cephalomannine ..............................................................................60 Miconazole ......................................................................................94 Chlorohydrine ..................................................................................55 Nevirapine .......................................................................................17 Chloride .........................................................................................100 Nitrate ............................................................................................100 Choline .............................................................................................24 Paclitaxel..........................................................................................60 Cortisol ............................................................................................65 Potassium .........................................................................................69 Cortisone ..........................................................................................65 Prednisolone ....................................................................................65 Deacetyl-epipaclitaxel .....................................................................60 Prednisone .......................................................................................65 Deoxycortisol ..................................................................................65 Propafenone .....................................................................................55 Dexamethasone ...............................................................................65 Propylene glycol ..............................................................................36 Epipaclitaxel ....................................................................................60