IKBKB polyclonal antibody Gene Summary: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM Catalog Number: PAB18155 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B Regulatory Status: For research use only (RUO) proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in Product Description: Rabbit polyclonal antibody raised the cytoplasm. Phosphorylation of serine residues on the against synthetic peptide of IKBKB. I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination Immunogen: A synthetic peptide corresponding to pathway, thereby allowing activation of the NF-kappa-B human IKBKB. complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such Host: Rabbit as 5-prime GGGRNNYYCC 3-prime or 5-prime Reactivity: Human,Mouse,Rat HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by Applications: ELISA, IHC-P, WB-Ce OMIM] (See our web site product page for detailed applications information) References: 1. NIBP, a novel NIK and IKK(beta)-binding protein that Protocols: See our web site at enhances NF-(kappa)B activation. Hu WH, Pendergast http://www.abnova.com/support/protocols.asp or product JS, Mo XM, Brambilla R, Bracchi-Ricard V, Li F, Walters page for detailed protocols WM, Blits B, He L, Schaal SM, Bethea JR. J Biol Chem. 2005 Aug 12;280(32):29233-41. Epub 2005 Jun 10. Specificity: This antibody is specific to IKBKB. 2. IKK beta plays an essential role in the phosphorylation of RelA/p65 on serine 536 induced by Form: Liquid lipopolysaccharide. Yang F, Tang E, Guan K, Wang CY. J Immunol. 2003 Jun 1;170(11):5630-5. Purification: Affinity purification 3. IKK beta is required for Bcl-2-mediated NF-kappa B activation in ventricular myocytes. Regula KM, Ens K, Concentration: 1 mg/mL Kirshenbaum LA. J Biol Chem. 2002 Oct Recommend Usage: Western Blot (1:500-1:1000) 11;277(41):38676-82. Epub 2002 Aug 7. Immunohistochemistry (1:50-1:100) ELISA (1:5000) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide) Storage Instruction: Store at -20°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 3551 Gene Symbol: IKBKB Gene Alias: FLJ40509, IKK-beta, IKK2, IKKB, MGC131801, NFKBIKB Page 1/1 Powered by TCPDF (www.tcpdf.org).