DNA/RNA Synthesis RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm. Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA. First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein. www.MedChemExpress.com 1 DNA/RNA Synthesis Inhibitors, Agonists, Activators, Modulators & Chemicals (+)-TK216 (-)-TK216 Cat. No.: HY-122903B Cat. No.: HY-122903A (+)-TK216 is an enantiomer of TK216 (HY-122903). (-)-TK216 is an enantiomer of TK216 (HY-122903). TK216 is an orally active and potent E26 TK216 is an orally active and potent E26 transformation specific (ETS) inhibitor. transformation specific (ETS) inhibitor. (-)-TK216 has anti-cancer activity. Purity: >98% Purity: 99.29% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 10 mM × 1 mL, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg Size: 10 mM × 1 mL, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg (S)-Crizotinib 1-Hydroxyanthraquinone Cat. No.: HY-100549 Cat. No.: HY-W000838 (S)-Crizotinib is a potent and selective MTH1 1-Hydroxyanthraquinone, a naturally occurring (mutT homologue) inhibitor with an IC50 of 330 compound with oral activity from some plants like nM. Tabebuia avellanedae, exhibits carcinogenic effect. Purity: 99.98% Purity: >98% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 10 mM × 1 mL, 2 mg, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg Size: 10 mM × 1 mL, 500 mg 2'-Azido-2'-deoxyuridine 2,4-D (N3dUrd) Cat. No.: HY-135957 (2,4-Dichlorophenoxyacetic acid) Cat. No.: HY-18572 2'-Azido-2'-deoxyuridine (N3dUrd) is a 2,4-D (2,4-Dichlorophenoxyacetic acid) is a ribonucleotide reductase inhibitor. selective systemic herbicide for the control of 2'-Azido-2'-deoxyuridine has anti-cancer activity. broad-leaved weeds. 2,4-D acts as a plant hormone, causing uncontrolled growth in the meristematic tissues. Purity: >98% Purity: ≥97.0% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 10 mM × 1 mL, 500 mg 2,4-D sodium salt (Sodium 2,4-dichlorophenoxyacetate; 2-(Methylamino)-1H-purin-6(7H)-one 2,4-Dichlorophenoxyacetic acid sodium salt) Cat. No.: HY-18572A (N2-methylguanine) Cat. No.: HY-101412 2,4-D sodium salt (Sodium 2-(Methylamino)-1H-purin-6(7H)-one 2,4-dichlorophenoxyacetate) is a selective (N2-Methylguanine) is a modified nucleoside. systemic herbicide for the control of broad-leaved 2-(Methylamino)-1H-purin-6(7H)-one is an weeds. 2,4-D sodium salt acts as a plant hormone, endogenous methylated nucleoside found in human causing uncontrolled growth in the meristematic fluids. tissues. Purity: >98% Purity: ≥98.0% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 5 mg, 10 mg, 50 mg, 100 mg 2-Keto-D-galactose 2-O-Methylcytosine (D-​Galactosone) Cat. No.: HY-136110 Cat. No.: HY-69014 2-Keto-D-galactose (D-Galactosone) inhibits DNA 2-O-Methylcytosine, an O-alkylated analogue a DNA synthesis, and inhibits proliferation of in vitro adduct, is the damaged nucleobase. grown Ehrlich ascites tumor cells. Purity: >98% Purity: ≥97.0% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 10 mM × 1 mL, 100 mg, 500 mg 2 Tel: 609-228-6898 Fax: 609-228-5909 Email: [email protected] 3'-Deoxyuridine-5'-triphosphate 3'-Deoxyuridine-5'-triphosphate trisodium (3'-dUTP) Cat. No.: HY-135780 (3'-dUTP trisodium) Cat. No.: HY-135780A 3'-Deoxyuridine-5'-triphosphate (3'-dUTP) is a 3'-Deoxyuridine-5'-triphosphate trisodium (3'-dUTP nucleotide analogue that inhibits DNA-dependent trisodium) is a nucleotide analogue that inhibits RNA polymerases I and II. DNA-dependent RNA polymerases I and II. 3'-Deoxyuridine-5'-triphosphate strongly and 3'-Deoxyuridine-5'-triphosphate trisodium strongly competitively inhibits the incorporations of UTP and competitively inhibits the incorporations of into RNA with a Ki value of 2.0 μM. UTP into RNA with a Ki value of 2.0 μM. Purity: >98% Purity: 98.05% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg Size: 1 mg 3,4-Dihydroxybenzylamine hydrobromide 3,7,4'-Trihydroxyflavone (NSC 263475 hydrobromide) Cat. No.: HY-N3023 (5-Deoxykampferol) Cat. No.: HY-111806 3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 3,7,4'-Trihydroxyflavone, isolated from Rhus hydrobromide) is an improved dopamine analog javanica var. roxburghiana, is a flavonoid with DNA cytotoxic and inhibits DNA polymerase activity strand-scission activity. in melanoma cells. Purity: >98% Purity: ≥98.0% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 10 mM × 1 mL, 5 mg, 10 mg, 25 mg 3-AP 4-Methoxyphenethyl alcohol (PAN-811; NSC# 663249; OCX191) Cat. No.: HY-10082 Cat. No.: HY-W004056 3-AP (PAN-811) is a potent inhibitor of the M2 4-Methoxyphenethyl alcohol, an aromatic alcohol, subunit of ribonucleotide reductase (RR), and is is the major component in the anise-like odour a potent radiosensitizer. produced by A. albispathus Hett. 4-Methoxyphenethyl alcohol can inhibits the protein, RNA and DNA synthesis in Escherichia coli. Purity: 99.31% Purity: >98% Clinical Data: Phase 3 Clinical Data: No Development Reported Size: 10 mM × 1 mL, 5 mg, 10 mg, 25 mg, 50 mg Size: 10 mM × 1 mL, 500 mg 5'-O-DMT-dT 5'-O-DMT-N4-Ac-dC (5'-O-(4,4'-Dimethoxytrityl)thymidine) Cat. No.: HY-20140 (N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine) Cat. No.: HY-W077279 5'-O-DMT-dT (5'-O-(4,4'-Dimethoxytrityl)thymidine) 5'-O-DMT-N4-Ac-dC is a nucleoside derivative which can be used in (N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine, compound the preparation of oligonucleotides. 7), a deoxynucleoside, can be used to synthesize of dodecyl phosphoramidite which is the raw material for dodDNA (amphiphilic DNA containing an internal hydrophobic region consisting… Purity: >98% Purity: >98% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 500 mg 5-Iminodaunorubicin hydrochloride 5-Methoxyflavone Cat. No.: HY-138645A Cat. No.: HY-107790 5-Iminodaunorubicin hydrochloride is a 5-Methoxyflavone, belonged to Flavonoid family, is quinone-modified anthracycline that retains a DNA polymerase-beta inhibitor and antitumor activity. 5-Iminodaunorubicin neuroprotective agent against beta-amyloid hydrochloride produces protein-concealed DNA toxicity. possess central nervous system (CNS) strand breaks in cancer cells. depressant effect mediated through the ionotropic GABAA receptors. Purity: >98% Purity: 99.98% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 10 mM × 1 mL, 25 mg www.MedChemExpress.com 3 5-Methylcytosine 5-Propargylamino-3'-azidomethyl-dCTP Cat. No.: HY-W008091 Cat. No.: HY-132138 5-Methylcytosine is a well-characterized DNA 5-Propargylamino-3'-azidomethyl-dCTP is a modification, and is also predominantly in nucleoside molecule extracted from patent abundant non-coding RNAs in both prokaryotes and WO2004018497A2, compound 17. eukaryotes. 5-Propargylamino-3'-azidomethyl-dCTP can be used in DNA synthesis and DNA sequencing. Purity: 99.82% Purity: >98% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 10 mM × 1 mL, 100 mg Size: 1 mg, 5 mg 5-Propargylamino-3'-azidomethyl-dUTP 5-Propargylamino-dCTP Cat. No.: HY-132137 Cat. No.: HY-132142 5-Propargylamino-3'-azidomethyl-dUTP is a 5-Propargylamino-dCTP is a nucleoside molecule nucleoside molecule extracted from patent extracted from patent US9035035B2, compound WO2004018497A2, compound 5. dCTP-PA. 5-Propargylamino-dCTP can conjugate to 5-Propargylamino-3'-azidomethyl-dUTP can be used molecular markers for use in nucleic acid labeling in DNA synthesis and DNA sequencing. or sequence analysis. Purity: >98% Purity: >98% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 1 mg, 5 mg 5-Propargylamino-ddCTP 5-Propargylamino-ddUTP Cat. No.: HY-132146 Cat. No.: HY-132145 5-Propargylamino-ddCTP, a nucleoside molecule that 5-Propargylamino-ddUTP, a nucleoside molecule that can be used to synthesis of cyanine dye-nucleotide can be used to synthesis of cyanine dye-nucleotide conjugate which is used in nucleic acid labeling conjugate which is used in nucleic acid labeling or sequence analysis. or sequence analysis. Purity: >98% Purity: >98% Clinical Data: No Development Reported Clinical Data: No Development Reported Size: 1 mg, 5 mg Size: 1 mg, 5 mg 6-Azathymine 6-Thio-2'-Deoxyguanosine Cat. No.: HY-136559 (6-thio-dG; β-TGdR) Cat. No.: HY-18762 6-Azathymine, a 6-nitrogen analog of thymine, is a 6-Thio-2'-Deoxyguanosine is a nucleoside analogue potent D-3-aminoisobutyrate-pyruvate that can be incorporated into de novo-synthesized aminotransferase inhibitor.