The Molecular Life of Diatoms 6 Virtually from San Diego, California July 12 -14, 2021 Program and Abstract Handbook Organizers Andrew Allen University of California, San Diego, Scripps Institution of Oceanography J. Craig Venter Institute, USA Co-Organizers Sarah Smitch Harriet Alexander Moss Landing Marine Laboratories, USA Woods Hole Oceanographic Institution Zoe Finkel Mariella Ferrante Stazione Zoologica Anton Dohrn of Naples Co-Organizers Chris Bowler Thomas Mock CNRS: French National Centre for University of East Anglia, UK Scientific Research Jesse Traller Hanhua Hu Global Algae Innovations, Inc., USA Chinese Academy of Science, China Tsuyoshi Tanaka Tokyo University of Agriculture & Technology, Japan Invited Speakers Tom Delmont Eveline Pinseel Genoscope, France University of Arkansas, USA Elena Litchman Erik Selander Michigan State University, USA University of Gothenburg Kim Thamatrakoln Bryndan Durham Rutgers University, USA University of Florida, USA Invited Speakers Shady Amin Uta Passow New York University Abu Dhabi Memorial University, Canada Yuichiro Kashiyama Katherine Helliwell Fukui University of Technology Marine Biological Association, Uni. of Exeter David Hutchins Sacha Coesel University of Southern California, USA University of Washington, USA Invited Speakers Atle Bones Gwenn Hennon NTNU: Norwegian University of Science University of Alaska Fairbanks, USA and Technology Stephanie Dutkiewicz Masao Adachi Massachusetts Institute of Technology, USA Kochi University, Japan Bogumil Karas Assaf Gal The University of Western Ontario, Canada Weizmann Institute of Science, Israel Invited Speakers Miroslav Oborník Graham Peers Biology Centre, CAS, Institute of Parasitology Colorado State University, USA Patrick Keeling Dmitry Filatov University of British Colombia, Canada University of Oxford, UK David Hutchins Sacha Coesel University of Southern California, USA University of Washington, Molecular Life of Diatoms 6 Conference Program Monday July 12 Tuesday July 13 Wednesday July 14 2:00 – 2:40pm GMT Welcome and Opening Remarks 2:00 – 2:20pm GMT Opening Remarks 2:00 – 2:20pm GMT Opening Remarks (Andy Allen) (Andy Allen) (Andy Allen) Session 1: Ecology & Ecophysiology Session 2: Ecophysiology & Cell Biology Session 3: Biotechnology & Evolution (Moderator: Harriet Alexander) (Moderator: Sarah Smith) (Moderator: Thomas Mock) 2:20 – 2:40pm GMT Kashiyama 2:20 – 2:40pm GMT Adachi How to digest delicious diatoms containing the phototoxic Activities of promoters derived from four diatom-infecting chlorophyll DNA viruses in the marine diatom Phaeodactylum tricornutum 2:40 – 3:00pm GMT Delmont 2:40 – 3:00pm GMT Helliwell 2:40 – 3:00pm Karas Environmental genomics of diatoms leads to new functional A novel Ca2+ signalling pathway coordinates environmental Introduction to Synthetic Diatom Project and evolutionary insights phosphorus sensing and nitrogen metabolism in marine diatoms 3:00 – 3:20pm GMT Pinseel 3:00 – 3:20pm GMT Hutchins 3:00 – 3:20pm GMT Gal Unravelling a natural evolutionary experiment: the Interactions between irradiance and temperature determine Using cryo electron microscopy to study diatom silicification colonization of low-salinity habitats in the Baltic Sea by the the niche of a novel low-light, cold-adapted nano-diatom marine diatom Skeletonema marinoi from a wintertime temperate estuary 3:20 – 3:40pm GMT Litchman 3:20 – 3:40pm GMT Coesel 3:20 – 3:40pm GMT Obornik Linking traits, evolution, and ecological interactions to Diel transcriptional oscillations of light-sensitive regulatory Exploring the heme pathway in chromerid and diatom understand diatom ecology elements in open ocean eukaryotic plankton communities 3:40 – 4:00pm GMT Selander 3:40 – 4:00pm GMT Bones 3:40 – 4:00pm GMT Peers (Cell Bio) Grazer induced phenotypic plasticity provides insight into Diatom Genomics and Gene Regulation Biosynthesis of fucoxanthin diatom evolution and molecular mechanisms 4:00 – 4:20pm GMT – Break 4:00 – 4:20pm GMT Hennon 4:00 – 4:20pm GMT Keeling (Evol) Diatoms on thin ice: sensitivity and resiliency to How does endosymbiosis lead to new plastids? anthropogenic change in the Alaskan Arctic 4:20 – 4:40pm GMT Thamatrakoln 4:20 – 4:40pm GMT Dutkiewicz 4:20 – 4:40pm GMT Filatov (Evol) Shunt or shuttle? Nutrient-driven biogeochemical Modeling marine diatom diversity and potential future Evolutionary genetics of marine phytoplankton: many consequences of diatom host-virus interactions changes at the global scale questions, few answers 4:40 – 5:00pm GMT Durham 4:40 – 5pm GMT Panel Discussion and Break 4:40 – 5pm Panel Discussion and Break Exploring cooperative nutrient dynamics in diatom-bacterial (Moderator: Mariella Ferrante) (Moderator: Jesse Traller) interactions 5:00 – 5:20pm GMT Amin 5:00 – 6:00pm: Special Session on Community Tools Highlighted Talks from Poster Presentations How diatoms modulate bacteria in their microbiome (Moderator: Chris Bowler) & Presentation of Poster Awards (Moderator: Andy Allen) 5:20 – 5:40pm GMT Passow 5:00 – 5:15pm GMT TBD Diatoms and the biological carbon pump 5:15 – 5:30pm GMT TBD 5:40 – 6pm GMT Panel Discussion and Break (Moderator: Zoe Finkel) 5:30 – 5:45am GMT TBD 5:45 – 6pm GMT TBD Poster Session 1 Poster Session 2 Poster Session 3 6 – 7:30pm GMT 6 – 7:30pm GMT 6 – 7:30pm GMT Ecology Cell Biology Biotechnology 1. Upwelling-associated phytoplankton display resistance 1. Scarless GFP-tagging of native genes in Thalassiosira 1. A functional PDAT encoded by unequally expressed to ocean acidification pseudonana allele enhances triacylglycerol accumulation under nitrogen starvation in Phaeodactylum tricornutum 2. Diving into metapopulation genomics of the Chaetoceros 2. Prevalence of trypsin in marine phytoplankton and their 2. High-efficiency transformation by electroporation and genus functions in the integrative nitrogen and phosphorus feasibility of CRISPR-Cas9-mediated gene editing in a centric signaling network diatom Chaetoceros muelleri 3. Growth-Stage Related Shifts in Diatom Endometabolome 3. Valve morphogenesis in Amphitetras antediluviana 3. The heterologous expression of a green alga Composition Sets the Stage for Heterotrophic Bacterial Ehrenburg plastocyanin in Phaeodactylum tricornutum improves cell Foraging growth under iron-deficient conditions 4. Exploring expression patterns of diatom silicon 4. Extracellular products from a flavobacterium induce 4. The physiological role and structural arrangement of the transporters under Si stress and competition transcriptional shifts of cell cycle and metabolism in a model mitochondria of Fistulifera solaris in the oil degradation diatom. process 5. Survivors of the Sea: Using Transcriptomics to Elucidate 5. An integrative experimental and environmental atlas of 5. An Analytical Approach for Understanding Light the Survival Strategies of Century Old Diatoms in Sediment diatom chloroplast transporters Modulation by Diatom Frustules 6. Uncovering the diversity and seasonal patterns of 6. Single-cell heterogeneity in response to environmental 6. Digital holographic microscopy of morphological changes algicidal bacteria in the Western English Channel stress reveals pathways of cell fate regulation in marine of diatom cellular contents induced by papain enzyme diatoms 7. Growth dynamics of epizoic Achnanthes elongata and 7. Investigating molecular mechanisms for phosphorus 7. Producing the SARS-CoV-2 Spike RBD antigen in the non-epizoic Psammodictyon panduriforme in co-cultures sensing in the marine diatom, Phaeodactylum tricornutum diatom Phaeodactylum tricornutum 8. The acquisition strategies of external bicarbonate by 8. Elucidating the microenvironment of mineral formation 8. A method for intact nuclei isolation from diatoms to three plasma membrane SLC4 transporters under changing within silica deposition vesicles in diatoms facilitate the application of high-throughput technologies levels of CO2 limitation in the diatom, Phaeodactylum tricornutum. Molecular Life of Diatoms 6 Conference Program Monday July 12 Tuesday July 13 Wednesday July 14 Poster Session 1 (continued) Poster Session 2 (continued) Poster Session 3 (continued) 9. Cancelled 9. Exocytosis of diatom silica involves extensive membrane 9. Chloroplast genome engineering as a tool to improve the disintegration productivity of monoterpenes in the diatom Phaeodactylum tricornutum 10. Deciphering interactions between the marine benthic 10. Light sensing under the sea: diatom photoreceptors in 10. Diatoms as promising hosts for monoterpene engineering diatom Seminavis robusta and its microbiome using a multi- Tara Oceans omics approach 11. Pole to pole pattern of biogeography and acclimation in 11. Comparative morphological and transcriptome analyses 11. Proteomic analysis of the oil bodies with different sizes natural populations of marine diatoms of valve plasticity induced under different salinity conditions in the marine diatom Fistulifera solaris for oil body- in a centric diatom Pleurosira laevis engineering 12. Microdiversity and co-occurrence of diatoms and 12. A FIB-SEM–based workflow to study diatoms light 12. Synthetic biology for the controlled production of high- bacterial associates along a latitudinal gradient in the North acclimation value compounds in Phaeodactylum tricornutum. Pacific 13. Transporters dependent regulation of photosynthesis in 13. Molecular drivers of sexual reproduction in Ecophysiology Phaeodactylum tricornutum Cylindrotheca Closterium 13. Essential amino acids in Lhcx proteins to confer the rapid 14. The potential role of extracellular