MASTER OF TECHNOLOGY IN Biotechnology CURRICULUM & SYLLABUS Semester I Code Course Title Contact Hrs./ Credit A Theory Wk L 3-1-0- 4 MBT - 101 Genetic Engineering (Core Subject) MBT - 102 Project Management 3-1-0 4 MBT - 103 Advanced Plant Biotechnology /Plant 3-1-0 4 Genetic Engineeri ng (Core Subject) MBT - 104 Numerical Analysis and Biostatistics / 3-1-0 4 Mathematics and Biostatistic s MBT - 115 3-1-0 4 Elective-I (A/B/C/D/E) 15-5-0 20 B Practic al MBT - 191 Genetic Engineering Lab 0-0-6 2 MBT - 192 Advanced Plant Biotechnology Laboratory / Enzyme 0-0-6 2 Technology Lab 0-0-12 4 MBT-193 Seminar I 0-0-3 1 Semester Total 15-8-12 25 Elective-I Code Course Title Contact Hrs./ Wk Cre dit MBT – 115A Genomics & Proteomics 3-1-0 4 MBT – 115B Advanced Biochemistry 3-1-0 4 MBT – 115C Bio-entrepreneurship and Management 3-1-0 4 MBT - 115D Advances in Bioreactor Design, Development and 3-1-0 4 Scale up MBT – 115E Biophysical Chemistry 3-1-0 4 1 MASTER OF TECHNOLOGY IN Biotechnology CURRICULUM & SYLLABUS Semester: II Code Course Title Contact Hrs./ Wk Cre dit A Theory L-T-P MBT -201 Advanced Bioinformatics (Core Subjec t) 3-1-0 4 MBT -202 Immunology (Core Subject) 3-1-0 4 MBT -203(A/B) Bioprocess Engineering & Technology / 3-1-0 4 Bio-processing Tec hnology MBT -204 Downstream Processing 3-1-0 4 MBT - 215 3-1-0 4 (A/B/C/D/E/F/G) Elective-II 15-5-0 20 B Practic al MBT - 291 Bioinformatics Laboratory 0-0-6 2 MBT – Downstream processing Lab/ Immunology 0-0-6 2 292(A/B/C) Lab / Animal Cell Culture Laboratory 0-0-12 4 MBT-294 Seminar II 0-0-3 1 Semester Total 15-7-12 29 Elective-II Code Course Title Contact Hrs./ Wk Cre dit MBT - 215A Animal Cell Culture and it’s application 3-1-0 4 MBT – 215B Nano Biot ec hnology 3-1-0 4 MBT – 215C Teaching Methodology in Tec hn ical 3-1-0 4 Edu cationn nn MBT - 215D Genomics & Proteomics 3-1-0 4 MBT – 215E Genetics & Cell Biology 3-1-0 4 MBT – 215F Bio-entrepreneurship and Management 3-1-0 4 MBT – 215G Agriculture Biot ec hnology 3-1-0 4 MBT – 215H Cell Biology 3-1-0 4 2 MASTER OF TECHNOLOGY IN Biotechnology CURRICULUM & SYLLABUS Semester: III Code Course Title Contact Hrs./ Wk Cre dit A Theory L-T-P MBT – 315 Elec tive-III 3-1-0 4 (A/B/C/D) MBT – 316 Elec tive-IV 3-1-0 4 (A/B/C/D) 6-2-0 8 B Sessional MBT - 391 Thesis -Part I (Presentation and Viva) - 15 Semester Total 6-2-0 23 Elective-III Code Course Title Contact Hrs./ Wk Cre dit MBT - 315 A Research Methodology and Tec hn ical Report 3-1-0 4 Writing MBT - 315 B Nanot ec hnology 3-1-0 4 MBT – 315 C Modeling and Simulation in Bioprocess 3-1-0 4 MBT – 315 D Pharmaceutical Biot echnology 3-1-0 4 Elective-IV Code Course Title Contact Hrs./ Wk Cre dit MBT - 316 A Biosafety, Ethics and IPR 3-1-0 4 MBT - 316 B Environmental Biotechnology 3-1-0 4 MBT – 316 C Bio pharmaceuticals 3-1-0 4 MBT – 316 D Advanced Instrumentation in Biot ec hnology 3-1-0 4 Semester: IV Code Course Title Contact Hrs./ Wk Cre dit B Sessional MBT - 491 Thesis-Part II (Report and Presentation) 0-0-0 20 MBT - 492 Project Viva/ Thesis Viva 0-0-0 5 MBT-481 Comprehensive viva voce - 3 Semester Total 0-0-0 28 3 MASTER OF TECHNOLOGY IN Biotechnology CURRICULUM & SYLLABUS Semester: I MBT-101: Genetic Engineering (Credit 4) Unit I Basics tools of Genetic Engineering DNA Structure and properties; Restriction Enzymes; DNA ligase, Klenow enzyme, T4 DNA polymerase, Polynucleotide kinase, Alkaline phosphatase; Cohesive and blunt end ligation; Linkers; Adaptors; Homopolymeric tailing; Labeling of DNA: Nick translation, Random priming, Radioactive and non-radioactive probes, Hybridization techniques: Northern, Southern and Colony hybridization, Fluorescence in situ hybridization; Chromatin Immunoprecipitation; DNA-Protein Interactions- Electromobility shift assay; DNaseI footprinting; Methyl interference assay; Chemical Synthesis of oligonucleotides. Unit II Cloning Vectors Plasmids; Bacteriophages; M13 mp vectors; PUC19 and Bluescript vectors, Phagemids; Lambda vectors; Insertion and Replacement vectors; Cosmids; Artificial chromosome vectors (YACs; BACs); Animal Virus derived vectors-SV-40; vaccinia/bacculo & retroviral vectors; Expression vectors; pMal; GST; pET-can be omitted vectors; Protein purification; His-tag; GST-tag; MBP-tag etc.; Intein-based vectors; Inclusion bodies; Methodologies to reduce formation of inclusion bodies; Baculovirus and pichia vectors system. Unit III Cloning Methodologies Insertion of Foreign DNA into Host Cells; Transformation; Construction of libraries; Isolation of mRNA and total RNA; cDNA and genomic libraries; cDNA and genomic cloning; Expression cloning; Jumping and hopping libraries; Southwestern and Far-western cloning; Protein-protein interactive cloning and Yeast two hybrid system; Phage display; Principles in maximizing gene expression. Unit IV PCR and Its Applications Primer design; Fidelity of thermostable enzymes; DNA polymerases; Types of PCR – multiplex, nested, reverse transcriptase, real time PCR, touchdown PCR, hot start PCR, colony PCR, cloning of PCR products; Tvectors; Proof reading enzymes; PCR in gene recombination; Deletion; addition; Overlap extension; and SOEing; Site specific mutagenesis; PCR in molecular diagnostics; Viral and bacterial detection; PCR based mutagenesis, Mutation detection: SSCP, DGGE, RFLP, Oligo Ligation Assay (OLA), MCC (Mismatch Chemical Cleavage, ASA (Allele-Specific Amplification), PTT (Protein Truncation Test), Sequencing methods; Enzymatic DNA sequencing; Chemical sequencing of DNA; Automated DNA sequencing; RNA sequencing. Unit V Applications of Genetic Engineering Introduction of DNA into mammalian cells; Transfection techniques; Gene silencing techniques; Introduction to siRNA; siRNA technology; Micro RNA; Construction of siRNA vectors; Principle and application of gene silencing; Gene knockouts and Gene Therapy; Creation of knockout mice; Disease model; Somatic and germ-line therapy- in vivo and ex-vivo; Suicide gene therapy; Gene replacement; Gene targeting; Transgenics; cDNA and intragenic arrays; Differential gene expression and protein array. 4 MASTER OF TECHNOLOGY IN Biotechnology CURRICULUM & SYLLABUS Text/References 1. S.B. Primrose, R.M. Twyman and R.W.Old; Principles of Gene Manipulation. 6th Edition, S.B.University Press, 2001. 2. J. Sambrook and D.W. Russel; Molecular Cloning: A Laboratory Manual, Vols 1-3, CSHL, 2001. 3. J. D. Watson et al.; Recombinat DNA, W.H. Freeman and Company 4. B. R. Glick and J.J. Pasternak ; Mlecular Biotecnology: Principles and Applications of Recombinant DNA, ASM press 5. D. M. Glover and B.D. Hames; DNA cloning: A Practical Approach, IRL Press. 6. Brown TA, Genomes, 3rd ed. Garland Science 2006 7. Selected papers from scientific journals. 8. Technical Literature from Stratagene, Promega, Novagen, New England Biolab etc. MBT 102 - Project Management (Credit 4) Unit I Project Planning: Project Management scenario: Project Asset – issues and problems; Gantt Chart & LOB; Network Analysis; PERT/ CPM, Resource Monitoring& Control. Unit II Project Buying: Projects Procurement Process, Life-cycle costing, Project cost reduction methods, Project Stores, Organization & HRD issues, Computerization. Unit III Investment Feasibility Studies : Managing Project Resources Flow; Project cost- Capital & Operating; Forecasting Income, Estimation of Investment & ROI, Project Evaluation, Finance Sources, Appraisal Process. Unit IV Issues in Project Management: Project Audit, Project Monitoring& MIS, Cost Control, Real Time planning, Intangibles. Unit V Project Management: Case Studies. 5 MASTER OF TECHNOLOGY IN Biotechnology CURRICULUM & SYLLABUS MBT – 103: Advanced Plant Biotechnology (Credit 4) Unit I Plant Tissue Culture: An overview Historical perspective; Totipotency; Organogenesis; Somatic embryogenesis; Artificial seed production; Micropropagation; Somaclonal variation; Androgenesis and its applications in genetics and plant breeding; Germplasm conservation and cryopreservation. Unit II Plant Genomics Identification of candidate genes using: genetic information (positional cloning); biochemical and expression analysis (microarray analysis, proteomics, metabolomics); Characterization and functional analysis of candidate genes using: transformation, mutant populations, knockout systems; Heterologous expression systems; Protein analysis. Unit III The Gene transfer Techniques for the production of Transgenic Overview of different gene transfer methods • Indirect Gene transfer Methods: structural features of Ti plasmid, mechanism of gene transfer to plants Integration of T-DNA into plant genome, Molecular events in Agrobacterium mediated gene transfer. • Direct gene transfer methods: Particle bombardment mediated transformation, Mechanism, Particle gun design, parameter for effective transformation; selicon carbide fiber mediated transformation and alternative methods. • Reporter genes, Selectable and scorable markers, Binary and Co-integrative vectors, Removal of marker genes, Applications and limitations of Agrobacterium gene transfer. • Plastid engineering: Introduction, importance, scope and technique. Unit IV Application of Genetic Engineering: Some Case studies Genetic Engineering for Herbicide resistance • Genetic Engineering for Biotic and Abiotic Stress Resistance/Tolerance • Genetic Engineering for Vitamins and other value addition compounds • Genetic Engineering for Production of pharmaceutically important compounds • Genetic Engineering for Bioenergy generation • Terminator technology Unit V Gene Silencing and Signal Transduction An overview: RNA virus in plants, virus induced