LINZ 2013 – EUSAAT 2013 – ESNATS Conference Volume 2, No. 2 ISSN 2194-0479 (2013) AlteProceedingsX Horst Spielmann: Session V: Welcome Consequences of the 2013 EU Marketing Ban for Cosmetics & Session I: NanoTox International Activities, Disease Models & Session VI: Human-on-a-chip Specific Endpoints of Toxicity Session II: 3Rs go 3D Session VII: Tox21 (TT21C) Session III: & Specific Endpoints ESNATS Conference: LINZ 2013 of Toxicity Use of Human Embryonic 18th European Congress on Alternatives to Animal Testing Stem Cells for Session VIII: Novel Toxicity Testing EUSAAT 2013 Implementing Approaches 15th Annual Congress of EUSAAT Alternatives into Basic Research Session IV: ESNATS Conference EU Dir 2010/63 & Embryonic Stem cell-based Novel Session IX: Ethical & Legal Aspects Alternative Testing Strategies Drugs, Vaccines & EU FP7 Projects www.eusaat.org altex_Linz_u1_u1.indd 1 26.8.2013 16:29:56 Uhr The organisers and the Scientific Committee wish to thank the following sponsors: Across Barriers GmbH DE-Saarbrücken Federal Ministry of Science & Research AT-Vienna AnimalfreeResearch CH-Bern InSphero AG CH-Schlieren BASF SE DE-Ludwigshafen Linz Tourism AT-Linz Biodeptronix LLC US-Chapel Hill MatTek IVLSL SK-Bratislava Biomimiq – a division of Aeon Astron Europe B.V. NL-Leiden Merck KGaA DE-Darmstadt Biopredic International FR-Rennes sbv IMPROVER – Systems Biology Verification CH-Neuchâtel British American Tobacco p.l.c. UK-London PRIMACYT Cell Culture Technology GmbH DE-Schwerin set - Foundation for the Promotion of Alternate & CAAT-EU DE-Konstanz Complementary Methods to Reduce Animal Testing DE-Frankfurt CellTool GmbH DE-Tutzing suonix group GmbH CH-Schöftland Computer Company AT-Linz TECOmedical Group CH-Sissach Federal Government of Upper Austria AT-Linz Vier Pfoten International - Four Paws International AT-Vienna Federal Ministry of Agriculture, Forestry, DE-Frankfurt/ Environment & Water Management AT-Vienna ZINSSER ANALYTIC GmbH Main Federal Ministry of Health AT-Vienna www.eusaat.org altex_Linz_u2_u2.indd 1 26.8.2013 16:30:17 Uhr LINZ 2013 Welcome address Dear friends and colleagues, on behalf of EUSAAT, the European Society for Alternatives to Animal Testing, I welcome you to the “EUSAAT 2013 - Linz 2013” congress, which is actually the 15th Annual Congress of EUSAAT and the 18th congress on alternatives in Linz. This year we are happy that the EU FP7 project ESNATS, European Stem cell-based Novel Alternative Testing Strategies, will hold its annual conference in 2013 in Linz during the EUSAAT 2013 conference. We are sure that the ESNATS conference will in 2013 add to making EUSAAT 2013 most attractive. On March 11, 2013 the vision most of us share finally became reality when the EU Commission announced the marketing ban for cosmetics testing in animals. As most of you will recall during the past 20 years at our congresses in Linz we have had long and fruitful discussions on all aspects of the increasing number of amend- ments of the EU Cosmetics Directive. The success in 2013 will stimulate our enthusiasm to search for and im- plement more non-animal methods in other areas of safety testing. Europe has been the international leader in making our first dream become reality. We will have to cooperate intensively with our colleagues in the USA, Japan and around the world to establish non-animal test approaches that are scientifically better than the current animal-based approaches. During the past two decades the “Linz-Congress” has emerged in Europe as one of the major scientific events in the field of the 3Rs. EUSAAT 2013 is hosting presentations, discussions and exchange of new ideas for the benefit of alternative methods to animal experiments. The Scientific Committee has identified the most important subjects related to the 3Rs which are of interest to scientists in Europe. The EUSAAT 2013 conference is focusing in oral and poster sessions on animal-free disease models, non-animal tools for basic biomedical research, -omics techniques and advanced 3D models including recent progress on developing a “human-on-a-chip”. EUSAAT 2013 is providing a forum for discussing the new EU Directive 2010/63 on the protection of ex- perimental animals, which is currently being implemented in EU member states. Colleagues from the EU Com- mission, from member states and from the animal welfare movement will discuss the high expectations that the public and the animal welfare movement have for improving the current situation on behalf of the experimental animals. The EUSAAT Board is happy that the number of sponsors of the EUSAAT congresses has increased over the years, since without their continuous support we would not be able to maintain the high scientific standard and to keep the congress fee low. Both elements are equally important in order to attract young scientists from all over Europe and beyond. Therefore the EUSAAT Board and Scientific Committee want to thank all of the sponsors of EUSAAT 2013 on behalf of the participants. Finally, we are also happy that the abstracts of the EUSAAT 2013 conference on the 3Rs are published in ALTEX Proceedings and we want to thank the editors of ALTEX that they have helped us to pave the way for future cooperation on behalf of implementing the 3Rs in Europe. I finally want to thank my colleagues on the EUSAAT Board and in the Scientific Committee for their continu- ous support in planning EUSAAT 2013. Horst Spielmann President of EUSAAT ALTEX Proceedings 2, 2/13, LINZ 2013 I altex_2013_Linz-I.indd 1 26.8.2013 15:51:34 Uhr LINZ 2013 Preliminary Program Sunday, 15.09.2013 13:00-13:15 Welcome Addresses Session I - International Activities, Disease Models & Human-on-a-chip 13:15-13:35 Hajime Kojima, National Institute of Health Sciences, JP-Tokyo Japanese project “ARCH-Tox” for the future chemicals management policy: research and development of in vitro and in vivo assays for internationally leading hazard assessment and test methods 13:35-13:55 Horst Spielmann, Freie Universität Berlin, DE-Berlin Peer review panel evaluation of the ROS Photosafety Assay 13:55-14:15 Mario Fabri, University of Cologne, DE-Cologne Vitamin D-dependent antimicrobial pathways in human macrophages 14:15-14:35 Günther Weindl, Freie Universität Berlin, DE-Berlin Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders 14:35-14:55 Eva-Maria Materne, TU Berlin, DE-Berlin A multi-organ-chip platform for long-term maintenance and substance testing of human tissue co-culture 14:55-15:15 Dmitry Sakharov, Scientific Research Centre Bioclinicum, RU-Moscow A dynamic multi-organ-chip containing enterocytes and hepatocytes for the absorption of orally administered drugs prediction and substance toxicity testing 15:15-16:30 Poster session I + coffee break and exhibition 16:30-17:00 Horst Spielmann, Freie Universität Berlin, DE-Berlin Willi Halle Memorial Lecture Session II – 3Rs go 3D 17:00-17:30 Ellen Fritsche, Leibniz Research Institute of Environmental Medicine, DE-Düsseldorf Human Neural Progenitor Cell (hNPC) Aging is mimicked by 3D Cultures in vitro: an ‘Adverse Outcome Pathway’ gains importance at susceptible life stages 17:30-17:45 Jens Kelm, InSphero AG, CH-Zürich 3-dimensional organotypic in vitro model systems for liver, cardiac and neuro safety assessment 17:45-18:00 Silvia Angeloni, CSEM SA, CH-Neuchâtel Blood-brain tumor and air-blood tissue barrier: two examples of how BioMEMS can improve the relevance of co-cultured in-vitro models of biological barriers 18:00-18:15 Frederique van Acker, TNO Triskelion, NL-Zeist Cerium oxide nanoparticles air exposure: a comparison study using a human 3D airway model, A549 and BEAS-2B cell lines 18:15-18:30 Nicky Slawny, 3D Biomatrix, Inc., US-Ann Arbor 3D spheroid cell cultures as models in drug discovery 18:30-18:45 Martha Liley. CSEM SA, CH-Neuchâtel Towards replacing in vivo tests of dental and orthopedic implants 19:15-20:45 Annual general assembly of EUSAAT II ALTEX Proceedings 2, 2/13, LINZ 2013 altex_2013_Linz-I.indd 2 26.8.2013 15:51:34 Uhr LINZ 2013 Monday, 16.09.2013 - special day (at the same time session III of the 18th European Congress on Alternatives to Animal Testing) ESNATS CONFERENCE - Preliminary Programme “Use of Human Embryonic Stem Cells for Novel Toxicity Testing Approaches” 09:00-09:10 Horst Spielmann, President of EUSAAT, Freie Universitaet Berlin, DE-Berlin Opening address 09:10-09:20 Jürgen Hescheler, Coordinator of ESNATS, University of Cologne, DE-Cologne Overview of the ESNATS project 09:20-10:00 Marcel Leist, University of Konstanz, DE-Konstanz The ESNATS hESC-based toxicity test battery 10:00-10:40 Jan Hengstler, IFADO, DE-Dortmund The ESNATS hESC-based toxicity biomarker identification study 10:40-11:00 Jan Hengstler, IFADO, DE-Dortmund Hepatocyte in vitro systems to study metabolism: stem cell derived hepatocytes as an alternative to primary hepatocytes 11:00-11:15 Coffee break 11:15-11:30 Joery de Kock, Vrije Universiteit Brussels, BE-Brussels hSKP-derived hepatocytes for toxicity testing 11:30-11:50 Sieto Bosgra, TNO, NL-Utrecht In vitro-in vivo extrapolation of prenatal (neuro)toxicity assay data by PBPK modeling 11:50-12:10 Agapios Sachinidis, University of Cologne, DE-Cologne Identification of thalidomide-specific transcriptomics and proteomics signatures during differentiation of human embryonic stem cells 12:10-12:30 Karl-Heinz Krause, University of Geneva, CH-Geneva Pluripotent stem cell-derived engineered neural tissues: what’s new? 12:30-13:45 Lunch and posters