2015 Catalog Contact and Ordering Information We need the following information to By mail: GenHunter Corporation process an order by fax, phone, or email: 624 Grassmere Park Drive Suite 17 ❑ Billing address Nashville, TN 37211 ❑ Shipping address By Phone: 800-311-8260 or 615-833-0665 ❑ Purchase order / Credit card number By Fax: 615-832-9461 ❑ Name and telephone number of By e-mail: [email protected] persons placing the order [email protected] ❑ Catalog number, description, and Online Ordering: www.GenHunter.com quantity ordered www.DifferentialDisplay.com Terms For your convenience, GenHunter accepts these credit cards: Net 30 days from the date of invoice in U.S. dollars. Conditions of Product Use All products are for research use only. GenHunter disclaims any responsibility for injury or damage that may be caused by the failure of the buyer or any other person to use these products in accordance with the conditions outlined herein. Pricing and Shipping Prices listed are only for the U.S., Canada, & other countries without s and are subject to change without notice. Find distributors outside the U.S. and Canada on page 92 or www.GenHunter.com/distributors. Products are shipped FOB Nashville, TN. Shipping charges are prepaid and added to your invoice. Orders requiring shipment in dry ice are delivered via Federal Express Priority Overnight, Monday through Thursday, unless otherwise requested. Warranty GenHunter warrants that the products will meet specifications listed. Free replacement of any non-conforming products will be made only if GenHunter is notified within 30 days of product receipt. Patent Information The mRNA Differential Display technology is covered by U.S. Patents 5,262,311; 5,599,672; 5,665,547; 5,965,409; Japanese Patent 2843675; and other issued or pending foreign patents exclusively licensed to GenHunter Corporation. Purchase of the RNAimage® and RNAspectra® kits from GenHunter Corporation comes with a sub-license to practice the differential display process. The AP-TAG® Receptor/Ligand Interaction Detection System is covered by US patents 5,554,499 and 5,801,000 licensed to GenHunter Corporation which holds world-wide exclusive rights to the AP-TAG® technology in the field of research applications. Purchase of an AP-TAG® Kit or any pAPtag vector comes with a limited, single-user and non-transferable sublicense for use in research applications only. No part of the kit or pAPtag plasmid vectors shall be disseminated, propagated or distributed outside the user’s own laboratory without written permission from GenHunter. The PCR process is covered by patents owned by Hoffman-La Roche Inc. No license or immunity under any other patent is either granted or implied by the sale of any GenHunter product. Cover photograph: Seashore of Bar Harbor, Maine (by Peng Liang). Catalog Design and Editing: David Turell, Jonathan Meade To our valued customers In 2012, we celebrated the twentieth year since the invention of the award-winning differential display technology. During this time, differential display (DD) has become the most widely used methodology to clone differentially expressed genes. In fact, the number of publications using differential display has exceeded the number of publications using all the other competitive methods. Despite the flurry of interest in DNA microarrays and next-gen sequencing, there have still been more scientists who have published the successful cloning of differentially expressed genes using DD than those who used other techniques. See the Technical Notes Section (page 64) which has more information on advantages of DD over microarray and other competing technologies. In 1998, in recognition of the great impact of DD technology, its inventors, Drs. Peng Liang and Arthur Pardee, were awarded the prestigious Molecular Bioanalytics GenHunter Corporation, Nashville, TN, USA prize by the German Society of Molecular Biology and Biological Chemistry (see page 6). experience and relentless pursuit of perfection in differential display, we are confident that you will be able to obtain more In addition to the sheer number of differentially expressed valuable information on gene expression profiling from us genes cloned by differential display, the biological functions than from any other methodology. of these genes are emerging. GenHunter Corporation is proud to have been the driving force in making this powerful Our AP-TAG® ligand/receptor interaction detection technology within easy reach of biomedical researchers technology (page 43-59) has revolutionized the way cell sur- throughout the world (see the representative references from face receptors and ligands are detected and cloned. This tech- our customers starting on page 71). nology may allow one to further functionally characterize secreted proteins or cell surface molecules cloned by DD or Since 1992, the year when differential display was invented, other methods. GenHunter Corporation has continued to improve, perfect and streamline the technology. Our complete line of Our PerfectWestern® Western blotting line of products innovative products centered around differential display (page 56-57; download more info at www.PerfectWestern.com) technology has made GenHunter synonymous with are designed to save money while producing superior western differential display. These kits include the RNApure® RNA blots. The specially designed PerfectWestern containers isolation system (page 11), our well known MessageClean® are available in 37 sizes and perfectly accommodate western kit for the removal of chromosomal DNA (page 12), the blots of any size: whole blots, half blots, and strips. They RNAimage® kit featuring the third generation of differential significantly reduce the volume of expensive antibodies display technology (page 16), our magic PCR-TRAP® required while still producing beautiful western blots! We cloning system for cloning the PCR amplified cDNA (page also offer pre-cut PerfectMembranes™ in 3 sizes & 30), the HotPrime® DNA probe labeling kit for optimized 4 materials, PerfectFilm™ ECL film, the PerfectRocker™, Northern blot analysis (page 35) and the ReversePrime® The Belly Dancer® shaker, The Belly Button® shaker, and cDNA labeling kit for reverse Northern blot analysis (page PerfectPipettors™. 37) of cDNA fragments identified by differential display. We continue to expand our world-wide market as the The completion of the human genome was undoubtedly a leader in differential display technology. great achievement, however, a much more daunting task is trying to interpret the genomic information in the context of The RNAspectra® Fluorescent Differential Display Kit biology. Because of you, our valued customers, differential (page 20-24) is the only non-radioactive differential display display has been and will continue to be the leading technol- system with similar sensitivity to that of isotope labeling. By ogy for deciphering genomic information for the benefit of translating the great success of our customers using our scientific knowledge and human health. reagent kits, GenHunter is also proud to offer our state-of-the-art Fluorescent Differential Display Service (page 26-29) with complete computerized automation in liquid Best wishes with gene hunting! dispensing and digital fluorescent data capturing with unsurpassed data reproducibility. With our years of From all of us at GenHunter Corporation 800-311-8260 www.GenHunter.com 1 For Orders or Tech Support Table of Contents About GenHunter Corporation .....................................................................................6 About the 1998 Molecular Bioanalytics Award............................................................7 About mRNA Differential Display.................................................................................8 Kits, Reagents, and Services for Differential Display 9 “GenHunter’s Complete System for Differential Display” Outline.....................................................10 RNA Isolation, Cleaning, and Quantification 11 RNApure® Reagent - for total RNA isolation............................................................................................11 RNA Loading Mix - for RNA denaturation and gel analysis....................................................................11 MessageClean® Kit - for removal of DNA contamination from RNA.....................................................12 Individual Components for MessageClean® Kit........................................................................12 MatureMessage™ cRNA Isolation Reagent - for purification of cytoplasmic RNA (cRNA) ................13 Gene Spec™ Micro-Spectrophotometers - for quantification of 1-50µL of RNA/DNA........................14 Differential Display Kits and Services 16 RNAimage® Kits - for radioactive Differential Display ...........................................................................16 Individual Components for RNAimage® Kits ...........................................................................18 Frequently asked questions about the RNAimage® / RNAspectra® Kits.............................................19 RNAspectra® Green Kits - for Fluorescent Differential Display (with Fluorescein) ..............................20 Individual Components for RNAspectra® Green Kits...............................................................24 RNAspectra® Red Kits - for Fluorescent Differential Display (with Rhodamine)..................................22 Individual Components for RNAspectra® Red Kits ..................................................................24