SANTA CRUZ BIOTECHNOLOGY, INC. MOV10 (B-3): sc-515722 BACKGROUND APPLICATIONS MOV10 (moloney leukemia virus 10), also known as putative helicase MOV10 (B-3) is recommended for detection of MOV10 of mouse, rat and MOV-10, gb110 or fSAP113, is a 1,003 amino acid novel telomerase-asso- human origin by Western Blotting (starting dilution 1:100, dilution range ciated protein that belongs to the DNA2/NAM7 helicase family and SDE3 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein subfamily. Localizing to mRNA-degrading cytoplasmic P bodies, MOV10 may (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution function as a RNA helicase that is required to mediate miRNA-guided mRNA range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution cleavage and RNA-mediated gene silencing by the RISC (RNA-induced silenc- range 1:30-1:3000). ing) complex. Highly expressed in ovary and testis, MOV10 is involved in Suitable for use as control antibody for MOV10 siRNA (h): sc-88358, MOV10 hepatitis virus (HDV) transcription and replication, and is known to interact d siRNA (m): sc-149516, MOV10 shRNA Plasmid (h): sc-88358-SH, MOV10 with eIF2C1, eIF2C2, eIF6, Dicer and TRBP2. MOV10 exists as three alterna- shRNA Plasmid (m): sc-149516-SH, MOV10 shRNA (h) Lentiviral Particles: tively spliced isoforms that are encoded by a gene located on human chro- sc-88358-V and MOV10 shRNA (m) Lentiviral Particles: sc-149516-V. mosome 1 and mouse chromosome 3. Following translation, MOV10 may become phosphorylated upon DNA damage by either ATM or ATR. Molecular Weight of MOV10: 110 kDa. Positive Controls: HeLa whole cell lysate: sc-2200, Hep G2 cell lysate: REFERENCES sc-2227 or Jurkat whole cell lysate: sc-2204. 1. Baird, D., et al. 2005. The Cool-2/a-Pix protein mediates a Cdc42-Rac signaling cascade. Curr. Biol. 15: 1-10. RECOMMENDED SUPPORT REAGENTS 2. Chendrimada, T.P., et al. 2007. MicroRNA silencing through RISC recruit- To ensure optimal results, the following support reagents are recommended: ment of eIF6. Nature 447: 823-828. 1) Western Blotting: use m-IgGk BP-HRP: sc-516102 or m-IgGk BP-HRP (Cruz Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ 3. Matsuoka, S., et al. 2007. ATM and ATR substrate analysis reveals extensive Molecular Weight Standards: sc-2035, UltraCruz® Blocking Reagent: protein networks responsive to DNA damage. Science 316: 1160-1166. sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- 4. Online Mendelian Inheritance in Man, OMIM™. 2007. Johns Hopkins cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). University, Baltimore, MD. MIM Number: 610742. World Wide Web URL: 3) Immunofluorescence: use m-IgGk BP-FITC: sc-516140 or m-IgGk BP-PE: http://www.ncbi.nlm.nih.gov/omim/ sc-516141 (dilution range: 1:50-1:200) with UltraCruz® Mounting Medium: sc-24941 or UltraCruz® Hard-set Mounting Medium: sc-359850. 5. Haussecker, D., et al. 2008. Capped small RNAs and MOV10 in human hepatitis d virus replication. Nat. Struct. Mol. Biol. 15: 714-721. DATA 6. Nakano, M., et al. 2009. MOV10 as a novel telomerase-associated protein. Biochem. Biophys. Res. Commun. 388: 328-332. ABCDE AB C D CHROMOSOMAL LOCATION 132 K – MOV10 132 K – < MOV10 Genetic locus: MOV10 (human) mapping to 1p13.2; Mov10 (mouse) mapping 90K– 90 K – to 3 F2.2. 55K– 55 K – SOURCE 43K– MOV10 (B-3) is a mouse monoclonal antibody raised against amino acids MOV10 (B-3): sc-515722.Western blot analysisof MOV10 (B-3): sc-515722.Western blot analysisof 1-57 mapping at the N-terminus of MOV10 of human origin. MOV10expressioninHeLa (A), Hep G2 (B), A-431(C), MOV10expressioninc4(A), NIH/3T3 (B), JC (C) and Jurkat (D) and NCI-H292 (E)wholecell lysates. NBT-II (D)wholecell lysates. PRODUCT SELECT PRODUCT CITATIONS Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. 1. Schöbel, A., et al. 2021. Hepatitis C virus infection restricts human LINE-1 retrotransposition in hepatoma cells. PLoS Pathog. 17: e1009496. MOV10 (B-3) is available conjugated to agarose (sc-515722 AC), 500 µg/ 0.25 ml agarose in 1 ml, for IP; to HRP (sc-515722 HRP), 200 µg/ml, for STORAGE WB, IHC(P) and ELISA; to either phycoerythrin (sc-515722 PE), fluorescein Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of (sc-515722 FITC), Alexa Fluor® 488 (sc-515722 AF488), Alexa Fluor® 546 shipment. Non-hazardous. No MSDS required. (sc-515722 AF546), Alexa Fluor® 594 (sc-515722 AF594) or Alexa Fluor® 647 (sc-515722 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; RESEARCH USE and to either Alexa Fluor® 680 (sc-515722 AF680) or Alexa Fluor® 790 (sc-515722 AF790), 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM. For research use only, not for use in diagnostic procedures. Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.