AAVLD Plenary Session Saturday, Oct 20, 2007 Ponderosa B “Past, present and future of veterinary laboratory medicine” Moderator: Grant Maxie 07:30 AM Welcome - Grant Maxie, President-Elect, AAVLD David Steffen, Vice-President, AAVLD 07:35 AM The evolution of the AAVLD - Robert Crandell, Larry Morehouse, Vaughn Seaton 08:15 AM Veterinary diagnostic toxicology: from spots to peaks to fragments and beyond (or why does diagnostic toxicology cause economic heartburn for laboratory directors?) - Robert Poppenga, Mike Filigenzi, Elizabeth Tor, Linda Aston, Larry Melton, Birgit Puschner 08:45 AM Microspheres and the evolution of testing platforms - Susan Wong 09:15 AM BREAK 09:45 AM A production management (client’s) perspective on diagnostics - Dale Grotelueschen 10:15 AM AAVLD survey of pet food-induced nephrotoxicity in North America, April to June, 2007 - Wilson Rumbeiha, Dalen Agnew, Grant Maxie, Michael Scott, Brent Hoff, Barbara Powers 10:45 AM Ecosystem health, agriculture, and diagnostic laboratories: challenges and opportunities - Thomas Besser 11:15 AM House of Delegates Virology Scientific Session Saturday, October 20, 2007 Bonanza A Moderators: Kyoung-Jin Yoon, Kristy Lynn Pabilonia 1:00 PM Further improvement and validation of MagMAX-96 AI/ND viral RNA isolation kit for efficient removal of RT-PCR inhibitors from cloacal swabs and tissues for rapid diagnosis of avian influenza virus by real-time reverse transcription PCR - Amaresh Das, Erica Spackman, Mary J. Pantin-Jackwood, David E. Swayne, David Suarez 1:15 PM Development of high-throughput real-time PCR procedure including an internal control for detection of PCV-2 in various biological samples - Wonil Kim, Karen Harmon, Tanja Opriessnig, Wendy Stensland, Kathryn Behrens, Kyoung-Jin Yoon 1:30 PM Real-time RT-PCR testing for PRRSV: does viral evolution outsmart molecular diagnostic capability? - Karen Harmon, Amy Chriswell, Kathryn Behrens, Raquel Hansell, Kyoung-Jin Yoon 1:45 PM Integrated high-throughput workflow for concurrent detection of North American and European strains of Porcine reproductive and respiratory syndrome virus by qRT-PCR - Angela M. Burrell, Weiwei Xu, Quoc Hoang, Roy C. Willis, Rohan Shah, Mangkey Bounpheng, Xingwang Fan 2:00 PM Development, optimization, and validation of a Classical swine fever virus real- time RT-PCR assay - Jill Bieker, August Eberling, Barbara Martin, Tammy Beckham 2:15 PM Comparison of 2 RNA extraction methods for Classical swine fever virus and Foot-and-mouth disease virus - Jessica M. Rowland, Kate R. Schumann, Barbara Martin, Tammy R. Beckham 2:30 PM A visual DNA chip for identification of different genotypes of Foot-and-mouth disease virus - Chu-Hsiang Pan, Ming-Hwa Jong, Parn-Hwa Chao, Lu-Yuan Liu, Ping Wu, Gordon B. Ward, Brenda C. Donahue, Mary A. Kenny, Ming Y. Deng 2:45 PM Multiplexed Foot-and-mouth disease virus DIVA assay - Julie Perkins, Alfonso Clavijo Anatomic Pathology Scientific Session Saturday, October 20, 2007 Bonanza BC Moderator: Bill Layton 1:00 PM Coxiella-like infection in psittacines - Hulimangala L. Shivaprasad, Santiago S. Diab, Robert Nordhausen, Maria Belen Cadenas, Edward Breitschwerdt 1:15 PM Neuritis in a white leghorn chicken: Marek’s disease or peripheral neuropathy? - Hulimangala L. Shivaprasad, Isabel Gimeno 1:30 PM Peracute mass mortality of western grebes due to underwater primary blast trauma - Nikos Gurfield, Judy St. Leger 1:45 PM Severe, soft-tissue mineralization in bullfrog larvae from wastewater treatment wetlands - Kevin Keel, Aina Ruiz, Aaron Fisk, John Maerz 2:00 PM An epizootic of stomatitis, rhinitis, tracheitis, and bronchopneumonia in captive white-tailed deer in Mississippi - Jim Cooley, Brittany Baughman, Larry Hanson, Steve Demarais 2:15 PM Detection of PrPCWD in rectal lymphoid tissues in postmortem and live Rocky Mountain elk (Cervus elaphus nelsoni), a possible preclinical test for CWD - Terry R. Spraker, Thomas Gidlewski, Kurt VerCauteren, Aru Balachandran, Randy Munger, Lynn Creekmore, Katherine O’Rourke 2:30 PM Detection of PrPCWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD - Terry Spraker, Thomas Gidlewski, Justin Greenlee, Delwyn Keane, Amir Hamir, Katherine O'Rourke 2:45 PM Plague in mountain lions (Puma concolor) from the greater Yellowstone area of Wyoming - Todd Cornish, Cynthia Tate, Rosemary Jaffe, Toni Ruth, Howard Quigley, Brian Parrie, Terry Kreeger, Amy Boerger-Fields, Ken Mills Molecular Diagnostics Scientific Session Saturday, October 20, 2007 Ponderosa A Moderator: Glenn Songer 1:00 PM Real-time PCR testing of pooled (1:5) fecal samples for MAP compared to HEYM culture - Robert H. Whitlock, Beverly L. Mangold, Susan McAdams, Terry Fyock, Raymond Sweeney, Ynte Schukken, Julie Smith, JoAnn Van Kessel, Ernest Hovingh, Jeff Karns, David Wolfgang, Todd Johnson 1:15 PM A streamlined workflow for rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine fecal samples by real-time PCR - Darcy A. Myers, Quoc Hoang, Rohan Shah, Ivonne M. Moon, R. Chris Willis, WeiWei Xu, Angela M. Burrell, Weiwen Ge, Mangkey Bounpheng, Xingwang Fang, Lee Effinger 1:30 PM Evaluation of the AnDiaTec ParaTub® Immunomagnetic Separation-PCR for the high-throughput detection of Mycobacterium avium subsp. paratuberculosis in bovine milk - Christoph Metzger-Boddien, Daryush Khaschabi, Sylvia Boddien, Stephanie Reinauer, Thomas Schlederer, Johannes Kehle 1:45 PM Development of a real-time PCR (rt-PCR) assay to detect Tritrichomonas foetus in cattle - Dawn M. Bueschel, Gregory P. Jillson, John A. Wenzel, Donnarae Saenz, Larry D. Stuart, R. Flint Taylor, Pascale M. Léonard 2:00 PM Pooled PCR to detect Tritrichomonas foetus in beef bulls - James A. Kennedy 2:15 PM Development and comparison of a fluorescent microsphere immunoassay with the virus neutralization test for the detection of antibodies to Equine arteritis virus - Yun Young Go*, Susan J. Wong, Adam Branscum, Valerie L. Demarest, Kathleen M. Shuck, Mary Lynne Vick, Jianqiang Zhang, William H. McCollum, Peter J. Timoney, Udeni B. R. Balasuriya 2:30 PM Comparison of 2 real-time RT-PCR assays for the detection of Equine arteritis virus nucleic acid in equine semen and tissue culture fluid - Zhengchun Lu*, Adam Branscum, Kathleen M. Shuck, Edward Dubovi, Peter J. Timoney, Udeni B.R. Balasuriya 2:45 PM A real-time PCR assay to detect single nucleotide polymorphisms at codon 171 in the prion gene for the genotyping of genetic susceptibility to scrapie in sheep - Jerome T. McKay, Tiffany A. Brigner, Brian E. Caplin, Kimberly S. McCurdy, Richard L. Forde * Graduate student presentation Bovine viral diarrhea virus Scientific Session Saturday, October 20, 2007 Ponderosa B Moderator: Steven Bolin 1:00 PM Persistent Bovine viral diarrhea virus infection in cervids in Colorado - Colleen Duncan*, Hana VanCampen, Ryan Miller, Mike Miller 1:15 PM A summary of test results from large-scale BVDV antigen ELISA testing performed in a private laboratory setting - John Lawrence, Chris McClure 1:30 PM BVDV antigen ELISA false-positives associated with Brahman cattle - John Lawrence, Rick Linscott, Edmond Martel 1:45 PM Interlaboratory comparison of diagnostic testing methods for Bovine viral diarrhea virus - Narda Huyke, Sabrina L. Swenson, Julia F. Ridpath 2:00 PM Improved BVDV-1b challenge model for evaluating efficacy of protection against clinical signs following acute infection - Julia F. Ridpath, John D. Neill, Ernst Peterhans 2:15 PM Utilization of multiple diagnostic tests to identify cattle with Bovine viral diarrhea virus infections and persistence of positive tests in persistently infected cattle - Robert W. Fulton, Bill J. Johnson, Bill E. Hessman, Julia Ridpath, Sanjay Kapil, Lurinda J. Burge, Barbara Braziel, Kira Kautz, Amy Reck 2:30 PM BVDV ear notch survey to reveal potential false-negatives associated with a mutation in the Erns glycoprotein - John Lawrence, Rick Linscott, Edmond Martel 2:45 PM Evaluation of the AnDiaTec BoVir real-time RT-PCR kit for the detection of BVDV in pooled bovine ear notch and blood samples - Stephanie Reinhauer, Georg Moesslacher, Gottfried Schoder, Christoph Metzger-Boddien, Johannes Kehle * Graduate student presentation Epidemiology/Molecular Diagnostics Scientific Session Sunday, October 21, 2007 Bonanza A Moderators: Francois Elvinger, Lindsay Oaks 08:00 AM Laboratory-based early animal disease detection utilizing a prospective space- time permutation scan statistic - Craig Carter, Agricola Odoi, Jeremy Riley, Jackie Smith, Tony Cattoi, Stu McCollum 08:15 AM Epidemiologic characteristics of outbreaks associated with changes in the Foot-and-mouth disease virus genome in the 2001 epidemic in Argentina - Andres M. Perez, Guido König, Mark C. Thurmond 08:30 AM FMD BioPortal: a system for global surveillance of foot-and-mouth disease - Mark C. Thurmond, Andres M. Perez, Zack Whedbee, Chunju Tseng, Hsinchun Chen, Daniel Zeng, Mike Ascher 08:45 AM The use and limitations of expert opinion data for foot-and-mouth disease surveillance - Rebecca B. Garabed*, Andres M. Perez, Wesley O. Johnson, Mark C. Thurmond 09:00 AM Risk factors associated with spatial distribution of foot-and-mouth disease in Nepal - Bimal K. Chhetri*, Andres M. Perez, Mark C. Thurmond 09:15 AM A high-resolution global hierarchical Bayesian model to predict foot-and- mouth disease presence - Rebecca B. Garabed*, Andres M. Perez, Wesley O. Johnson, Mark C. Thurmond 09:30 AM Spatial and temporal characterization of animal