Abstracts of papers presented at the 2011 meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE September 6–September 10, 2011 View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Cold Spring Harbor Laboratory Institutional Repository Cold Spring Harbor Laboratory Cold Spring Harbor, New York Abstracts of papers presented at the 2011 meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE September 6–September 10, 2011 Arranged by Stephen Bell, HHMI / Massachusetts Institute of Technology Joachim Li, University of California, San Francisco Johannes Walter, Harvard Medical School Cold Spring Harbor Laboratory Cold Spring Harbor, New York This meeting was funded in part by the National Cancer Institute, a branch of the National Institutes of Health; and the National Science Foundation. Contributions from the following companies provide core support for the Cold Spring Harbor meetings program. Corporate Sponsors Agilent Technologies Astellas-OSI Oncology AstraZeneca BioVentures, Inc. Bristol-Myers Squibb Company Genentech, Inc. GlaxoSmithKline Life Technologies (Invitrogen & Applied Biosystems) New England BioLabs, Inc. Sanofi-Aventis Plant Corporate Associates Monsanto Company Pioneer Hi-Bred International, Inc. Foundations Hudson-Alpha Institute for Biotechnology ________________________________________________________________ Cover: Sulfolobus Orc1-1 and Orc1-3 bound to DNA. Photo courtesy of James Berger. EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE Tuesday, September 6 – Saturday, September 10, 2011 Tuesday 7:30 pm 1 New Approaches and Views of Replication Wednesday 9:00 am 2 Origin Selection and pre-RC Assembly Wednesday 2:00 pm 3 Poster Session I Wednesday 4:30 pm Wine and Cheese Party * Wednesday 7:30 pm 4 Response to Replication Stress and Damage Thursday 9:00 am 5 Origin Activation and Timing Thursday 2:00 pm 6 Poster Session II Thursday 7:30 pm 7 Chromatin, Cancer and Development Friday 9:00 am 8 Replisome Activities and Functions Friday 2:00 pm 9 Cell Cycle Control and Connections Friday 6:00 pm Concert 7:00 pm Banquet Saturday 9:00 am 10 Postreplicative Repair and Lesion Bypass Poster sessions are located in Bush Lecture Hall * Airslie Lawn, weather permitting Mealtimes at Blackford Hall are as follows: Breakfast 7:30 am-9:00 am Lunch 11:30 am-1:30 pm Dinner 5:30 pm-7:00 pm Bar is open from 5:00 pm until late Abstracts are the responsibility of the author(s) and publication of an abstract does not imply endorsement by Cold Spring Harbor Laboratory of the studies reported in the abstract. These abstracts should not be cited in bibliographies. Material herein should be treated as personal communications and should be cited as such only with the consent of the author. Please note that recording of oral sessions by audio, video or still photography is strictly prohibited except with the advance permission of the author(s), the organizers, and Cold Spring Harbor Laboratory. Printed on 100% recycled paper. PROGRAM TUESDAY, September 6—7:30 PM SESSION 1 NEW APROACHES AND VIEWS OF REPLICATION Chairpersons: D. MacAlpine, Duke University Medical Center, Durham, North Carolina D. Sherratt, University of Oxford, United Kingdom The Drosophila DNA replication program—Genome-wide distribution and dynamics of the MCM complex David M. MacAlpine, Sara K. Powell, Heather K. MacAlpine, Matthew L. Eaton, Joseph A. Prinz. Presenter affiliation: Duke University, Durham, North Carolina. 1 Interrogation of DNA replication using iPOND and a proximal biomarker of ATR activation Bianca M. Sirbu, Edward A. Nam, Frank B. Couch, David Cortez. Presenter affiliation: Vanderbilt University School of Medicine, Nashville, Tennessee. 2 Chromatin and the lagging strand Duncan Smith, Sean McGuffee, Iestyn Whitehouse. Presenter affiliation: Sloan-Kettering Institute, New York, New York. 3 Mammalian telomeres use telomere-specific, rather than universal, replication programs W. C. Drosopoulos, S. T. Kosiyatrakul, Z. Yan, S. G. Calderano, C. L. Schildkraut. Presenter affiliation: Albert Einstein College of Medicine, Bronx, New York. 4 Expanded GAA repeats induce atypical post-replicative DNA junctions in human cells Cindy Follonier, Massimo Lopes. Presenter affiliation: Institute of Molecular Cancer Research, Zürich, Switzerland. 5 Replisome dynamics in vivo David Sherratt, Mark Leake, Christian Lesterlin, Yichao Dong, David Brown, Rodrigo Reyes-Lamothe. Presenter affiliation: Oxford University, Oxford, United Kingdom. 6 v Quantitative proteomics reveals the functions of replication factor C-like complexes Takashi Kubota, Shin-ichiro Hiraga, Kayo Yamada, Angus I. Lamond, Anne D. Donaldson. Presenter affiliation: University of Aberdeen, Aberdeen, United Kingdom. 7 Local and origin specific determinants modulate global DNA replication controls Christopher Richardson, Joachim Li. Presenter affiliation: University of California, San Francisco, California. 8 Signaling roles for ribonucleotides in the S. cerevisiae nuclear genome Thomas A. Kunkel, Jessica S. Williams, Danielle L. Watt, Anders R. Clausen, Mercedes E. Arana, Alan B. Clark. Presenter affiliation: NIEHS, NIH, Research Triangle Park, North Carolina. 9 Extrachromosomal microDNAs and chromosomal microdeletions in normal tissues Yoshiyuki Shibata, Pankaj Kumar, Ryan Layer, Smaranda Willcox, Jack D. Griffith, Anindya Dutta. Presenter affiliation: University of Virginia, Charlottesville, Virginia. 10 WEDNESDAY, September 7—9:00 AM SESSION 2 ORIGIN SELECTION AND PRE-RC ASSEMBLY Chairpersons: J. Blow, University of Dundee, United Kingdom M. Debatisse, Institut Curie-CNRS-UPMC, Paris, France The dynamics of replication licensing in living C. elegans and Xenopus embryos Remi Sonneville, Jolanta Kisielewska, Matthieu Querenet, Ashley Graig, Anton Gartner, J. Julian Blow. Presenter affiliation: University of Dundee, Dundee, United Kingdom. 11 In vitro assembly of human pre-replicative complex and its regulation Min Wu, Wenyan Lu, Mark G. Frattini, Ruth Santos, Thomas J. Kelly. Presenter affiliation: Memorial Sloan Kettering Cancer Center, New York, New York. 12 vi Structure and mechanism in MCM2-7 loading during licensing of eukaryotic DNA replication Christian Speck, Jingchuan Sun, Juergen Zech, Carmen Herrera, Pippa Clarke, Stefan Samel, Bruce Stillman, Rudi Lurz, Hulin Li. Presenter affiliation: MRC-CSC / Imperial College, London, United Kingdom. 13 Replicators as chromatin organizers—Insights from replicator binding protein complexes and whole genome replication initiation maps Haiqing Fu, Melvenia M. Martin, Liang huang, Ya Zhang, Chii Mei Lin, Amy L. Conner, Mirit I. Aladjem. Presenter affiliation: National Cancer Institute, Bethesda, Maryland. 14 Genome-wide mapping of pre-RC binding sites and initiation sites in the EBV-genome reveals conserved features but also differences Peer Papior, Jose M. Arteaga-Salas, Aloys Schepers. Presenter affiliation: Helmholtz Zentrum München, Munich, Germany. 15 Replication profiles and fragility of common fragile site O Brison, D Azar, A Letessier, M Debatisse. Presenter affiliation: Institut Curie-CNRS-UPMC, Paris, France. 16 DNA replication origins—Conserved features and organization in metazoans Christelle Cayrou, Philippe Coulombe, Olivier Ganier, Alice Vigneron, Aurore Puy, Slavitza Stanojcik, Sabine Laurent-Chabalier, Isabelle Peiffer, Marcel Méchali. Presenter affiliation: Institute of Human Genetics, CNRS, Montpellier, France. 17 Identification and characterization of shared chromatin architecture at S. pombe origins of replication Robert M. Givens, William K. Lai, Jonathan E. Bard, Piotr A. Mieczkowski, Janet Leatherwood, Joel A. Huberman, Michael J. Buck. Presenter affiliation: Roswell Park Cancer Institute, Buffalo, New York. 18 Sequential and increasing activation of replication origins along replication timing gradients in the human genome Aurélien Rappailles, Guillaume Guilbaud, Antoine Baker, Chun-Long Chen, Benoit Moindrot, Antoine Leleu, Cedric Vaillant, Alain Arneodo, Arach Goldar, Yves d'Aubenton-Carafa, Claude Thermes, Benjamin Audit, Olivier Hyrien. Presenter affiliation: ENS-CNRS UMR8197, Paris, France. 19 vii Whole genome maps of origins of replication in a near-normal human lymphoblastoid cell line Larry D. Mesner, Veena Valsakumar, Stefan Bekiranov, Joyce L. Hamlin. Presenter affiliation: University of Virginia School of Medicine, Charlottesville, Virginia. 20 WEDNESDAY, September 7—2:00 PM SESSION 3 POSTER SESSION I NCC—A new technology to dissect chromatin dynamics at replication forks Constance Alabert, Zuzana Jasencakova, Flavia Alves, Jakob Mejlvang, Juri Rappsilber, Anja Groth. Presenter affiliation: Biotech Research and Innovation Centre (BRIC), Copenhagen, Denmark. 21 Rb-Independent TGF-beta1 cell cycle arrest and Inhibition of MCM hexamer assembly is abrogated by the replication oncoprotein Cdt1 Piyali Mukherjee, Reeti Behera, Mark G. Alexandrow. Presenter affiliation: Moffitt Cancer Center, Tampa, Florida. 22 Mcm10 functions with the 9-1-1 clamp in resistance to topoisomerase I-DNA adducts Robert C. Alver, Anja K. Bielinsky. Presenter affiliation: University of Minnesota, Minneapolis, Minnesota. 23 Investigating the properties of a repair replication-fork in the budding yeast Saccharomyces cerevisiae Ranjith P. Anand, James E. Haber. Presenter affiliation: Brandeis University, Waltham, Massachusetts. 24 New views on strand asymmetry in fission yeasts Benoit J. Arcangioli, Sophie Thomain. Presenter affiliation: Pasteur Institute, Paris, France. 25 viii Physical and functional interaction