The Effects of Cerulenin, an Inhibitor of Protein Acylation, on the Two Phases of Glucose-Stimulated Insulin Secretion Susanne G. Straub,1 Hiroki Yajima,2 Mitsuhisa Komatsu,2 Toru Aizawa,2 and Geoffrey W.G. Sharp1 The potential role of protein acylation in the control of sparse. It is known that glucose metabolism is essential for biphasic insulin secretion has been studied in isolated their operation, but the link between glucose metabolism rat pancreatic islets. The protein acylation inhibitor and increased exocytosis has not been established. Some cerulenin inhibited both phases of glucose-stimulated potential mechanisms for glucose action include: insulin secretion. However, it did not affect the secre- tory response to a depolarizing concentration of KCl in ● either the absence or presence of diazoxide. Therefore, A glucose-induced increase in malonyl-CoA, subsequent -2؉ inhibition of carnitine palmitoyl transferase I and de cerulenin has no deleterious effect on the L-type Ca channels or subsequent events in Ca2؉ stimulus–secre- creased fatty acid oxidation, and an increase in cytosolic tion coupling. Advantage was taken of this to study the long-chain acyl-CoAs. Increased cytosolic long-chain effect of cerulenin on the KATP channel–independent acyl-CoAs have the potential to act as second messen- pathway of glucose signaling. In the presence of KCl and gers per se or possibly activate second messengers like diazoxide, cerulenin powerfully inhibited the augmen- PKC isoforms. They may also act via protein acylation. tation of insulin release by glucose and palmitate. Sim- This “malonyl-CoA hypothesis” has a great deal of ilar inhibition of the augmentation of release by glucose supportive evidence (7–10) but is controversial because 2؉ and palmitate was seen under Ca -free conditions in of recent studies (11,12). the presence of 12-O-tetradecanoylphorbol-13-acetate ● The glucose-induced increase in the ATP/ADP ratio (13). and forskolin. As neither glucose oxidation nor the ● A putative glutamate signal (14), which is also contro- effect of glucose to inhibit fatty acid oxidation is af- fected by cerulenin, these data suggest that protein versial (15). ● Cytosolic NADPH produced by the mitochondrial pyru- acylation is involved in the KATP channel–independent pathway of glucose signaling. Diabetes 51 (Suppl. 1): vate–malate shuttle (16). S91–S95, 2002 The KATP channel–independent pathways are likely to consist of multiple signals that are coordinated with one another and with the KATP channel–dependent pathway. lucose stimulus–secretion coupling in the pan- 2ϩ An increase in [Ca ]i alone will stimulate insulin release, creatic ␤-cell can be described in terms of two as, for example, with sulfonylureas or depolarizing con- major signaling pathways. The KATP channel– centrations of KCl. However, the responses to these stim- dependent pathway acts via closure of K G ATP uli are different from the response to glucose. All cause a channels with subsequent depolarization of the cell, acti- 2ϩ peak of first-phase release, but only glucose results in a vation of voltage-dependent Ca channels, increased in- prominent second phase, pointing to the involvement of flux of Ca2ϩ, elevated cytosolic free Ca2ϩ concentration, 2ϩ the KATP channel–independent pathways in the generation [Ca ]i, and increased insulin release (1,2). The KATP of the second phase of release. Furthermore, as the first channel–independent pathways discovered in 1992 (3–5) phase is thought to be due to a small pool of readily act in synergy with the KATP channel–dependent pathway 2ϩ releasable granules, the second phase must be due to to augment the release induced by elevated [Ca ]i. They 2ϩ granules that have translocated from reserve pools to a also operate in the absence of extracellular Ca if protein releasable pool at the membrane. Therefore, in the pres- kinase C (PKC) and protein kinase A are activated (6). The 2ϩ ence of elevated [Ca ]i, the KATP channel–independent knowledge of the underlying mechanisms by which these pathways are responsible for the selection and transloca- KATP channel–independent pathways exert their effects is tion of insulin-containing granules from the reserve pools to the cell membrane, their assembly at the plasma mem- brane, priming to achieve fusion competence, and finally From the 1Department of Molecular Medicine, College of Veterinary Medicine, exocytosis. Cornell University, Ithaca, New York; and the 2Department of Aging Medicine In the present work, we have chosen to study the and Geriatrics, Shinshu University School of Medicine, Matsumoto, Japan. Address correspondence and reprint requests to [email protected]. potential signaling role of protein acylation on the dy- Accepted for publication 14 June 2001. namic aspects of glucose stimulus–secretion coupling. We BCH, 2-aminobicyclo(2.2.1)heptane; PKC, protein kinase C; SNAP-25, syn- investigated the effects of cerulenin, an inhibitor of protein aptosomal-associated protein-25; TPA, 12-O-tetradecanoylphorbol-13-acetate. The symposium and the publication of this article have been made possible acylation, on the two phases of glucose-stimulated insulin by an unrestricted educational grant from Servier, Paris. release and on the KATP channel–independent pathway in DIABETES, VOL. 51, SUPPLEMENT 1, FEBRUARY 2002 S91 PROTEIN ACYLATION AND THE CONTROL OF INSULIN RELEASE FIG. 1. The stimulation of insulin release by 16.7 mmol/l glucose (added at minute 10) (F)(A) and a combination of 10 mmol/l BCH and 10 mmol/l glutamine (added at minute 5) (F)(B), and the inhibitory effect of 100 ␮mol/l cerulenin (Ⅺ). The basal glucose concentration was 2.8 mmol/l. .4 ؍ Cerulenin was present only during the preincubation period. Mean ؎ SE, n the presence and absence of extracellular Ca2ϩ. Cerulenin the first phase suggested that the operation of either the 2ϩ inhibits glucose-stimulated insulin release maximally at KATP channels or voltage-dependent Ca channels had 100 ␮mol/l without affecting glucose or palmitate oxida- been compromised. To determine if this was the case, the tion, or ATP content at low and high glucose concentra- effect of cerulenin on the response to a depolarizing tions (17). However, until now there has been no dynamic concentration of KCl was studied. As can be seen from the analysis of the effects on biphasic release. results in Fig. 2, cerulenin had no effect on the response to KCl. Thus, the Ca2ϩ channels are operating normally in RESEARCH DESIGN AND METHODS response to depolarization, and the subsequent secretory Isolation of rat pancreatic islets and measurement of insulin secretion. 2ϩ response to elevated [Ca ]i is also unaffected. It is Male Wistar rats weighing 300–400 g were killed by CO asphyxiation, the 2 concluded that cerulenin either prevents closure of the pancreata were surgically removed, and islets were isolated by collagenase digestion in HEPES-buffered Krebs-Ringer bicarbonate solution (18,19). The KATP channels in response to nutrient stimulation or is islets were then subjected to a 45-min static incubation at 37°C in the presence actually activating the channels. Because of this effect, and or absence of 100 ␮mol/l cerulenin before they were transferred to perifusion because the KATP channel–independent pathway acts in chambers. A 40-min equilibration period was followed by perifusion under the synergy with increased [Ca2ϩ] , it was necessary to study indicated conditions, in which samples for insulin measurement were taken. i Release rates were determined by radioimmunoassay using a charcoal sepa- the effect of cerulenin on the KATP channel–independent ration technique (20). Since it has been shown that cerulenin covalently pathway in isolation. This was achieved by activating the modifies sulfhydryl residues contained in the active sites of enzymes involved KATP channels with diazoxide, depolarizing the cell with in fatty acid metabolism and therefore cannot be easily washed out (21), we KCl, and then measuring the effect of 16.7 mmol/l glucose added the compound only during the 45-min static incubations and not during the subsequent perifusion period. There was no evidence of reversibility of the on insulin release independently of the KATP channels. The effect of cerulenin over 90 min (21). results are shown in Fig. 3. The control response to 40 mmol/l KCl in the presence of 250 ␮mol/l diazoxide was RESULTS monophasic, with the peak secretion rate after 1 min and In initial control studies, 100 ␮mol/l cerulenin had no a return to basal levels after 10–15 min. The KATP channel– effect on the rate of basal insulin secretion in the presence independent response to glucose under these conditions or absence of extracellular Ca2ϩ (data not shown). This was a large increase in release over and above that of KCl concentration of the inhibitor was then used throughout alone. Peak rates were achieved after 10 min, and insulin the study. When insulin secretion was stimulated by secretion remained at high levels throughout the course of glucose or the combination of 10 mmol/l glutamine and 10 the experiment. Cerulenin strongly inhibited this response. mmol/l 2-aminobicyclo(2.2.1)heptane (BCH; a nonmetabo- The initial rise in insulin secretion to the KCl peak at 1 min lizable analog of leucine, which activates glutamate dehy- was unaffected. However, from minute 2, the secretion drogenase), cerulenin had a powerful inhibitory effect rate began to decline as the glucose response was inhib- (Fig. 1A and B, respectively). Both the first and second ited. Thus, cerulenin inhibits the KATP channel–indepen- phases of release were profoundly inhibited. The effect on dent pathway of glucose signaling. Because the main S92 DIABETES, VOL. 51, SUPPLEMENT 1, FEBRUARY 2002 S.G. STRAUB AND ASSOCIATES FIG. 2. The effects of 40 mmol/l KCl (f) (added at minute 10) to FIG. 4. The effects of 40 mmol/l KCl (᭹) on insulin release, and the lack stimulate insulin release, and the lack of inhibition of this response by of effect of cerulenin (100 ␮mol/l) (⅜) on KCl-induced secretion.