Scientific Proceedings 16TH ANNUAL RESEARCH DAY JANUARY 31, 2015 HILTON FORT COLLINS 2015 CVMBS-ZOETIS EARLY CAREER RESEARCH AWARD Two researchers in the College of Veterinary Medicine and Biomedical Sciences have been honored with the 2015 CVMBS-Zoetis Early ON THE COVER: Career Research Award. The honorees, selected Colorado State University by colleagues and peers, are Dr. Candace Mathiason graduate student Genevieve and Dr. Elizabeth Ryan. Each will receive a plaque Forster, working alongside Dr. Elizabeth Ryan, is among and $1,000 honorarium. The two awardees also will more than 140 undergradu- kick off 2015 Research Day by presenting talks ate students, graduate students, veterinary resi- about their work. In keeping with a proud tradition, dents, and post-doctoral global animal health company Zoetis sponsors fellows participating in 2015 Research Day. The day gives Research Day and the Early Career Research Awards. trainees in the College of Veterinary Medicine and Biomedical Sciences a showcase for their research efforts and findings. ZOETIS | EARLY CAREER RESEARCH AWARD WINNER Mathiason investigates prion disease to shed light on human neurologic diseases DR. CANDACE MATHIASON is an assistant professor and member of the Prion Research Center in the Department of Microbiology, Immunology, and Pathology. She established her research program in 2012. Her talk at 2015 Research Day is titled, “Stealthy Prions.” Mathiason received a Ph.D. in prion pathobiology from Colorado State University under the mentorship of Dr. Edward Hoover, University Distinguished Professor and member of the National Academy of Sciences. She had earlier worked as manager of the Hoover Laboratory, training in retrovirology and prion biology. The Mathiason Laboratory investigates the biological mechanisms associated with covert prion infection and transmission. Studies examine chronic wasting disease (CWD) prions in native cervid hosts using novel in vitro detection methodologies. This work is designed to provide insight for all transmissible spongiform encephalop- athies (TSEs), along with human neurologic diseases similar to prion diseases, including Alzheimer’s, Huntington’s, and Parkinson’s diseases, as well as type II diabetes. Animal prion diseases, including CWD, are remarkably similar to human prion diseases, re- capitulating trafficking, dissemination, and even transmission patterns. In exposed mammals, including humans, prions establish and maintain a silent carrier, or asymptomatic phase of disease, that lasts many months or years prior to the development of clinical TSE disease. Mathiason collaborates with researchers from Colorado, Nebraska, Kansas, Montana, Illinois, Wisconsin, New York, Georgia, Tennessee, Canada, Scotland, France, England, and Germany. She is a board member for the Rocky Mountain Virology Association and has played key roles in several regional, national and international conferences addressing prion and TSE research. Mathiason also mentors young scientists seeking to develop careers and is an alternate member on the CSU Institutional Animal Care and Use Committee. Mathiason’s research is funded by the U.S. Department of Agriculture, Animal and Plant Health In- spection Service; and National Institutes of Health, National Institute of Allergy and Infectious Diseases. SCIENTIFIC PROCEEDINGS 1 ZOETIS | EARLY CAREER RESEARCH AWARD WINNER Ryan examines rice bran as a route to reducing cancer and diarrheal disease DR. ELIZABETH RYAN is an assistant professor of toxicology in the Colorado State Uni- versity Department of Environmental and Radiological Health Sciences. She established her CSU research laboratory in 2010. Her talk at 2015 Research Day will be, “Rice bran: cross-kingdom and One Health perspectives.” Ryan received her doctorate in molecular toxicology from the University of Rochester School of Medicine, Department of Environmental Medicine. She received postdoctoral training with a National Cancer Institute fellowship in clinical cancer control and prevention and focused her research in the field of immunotoxicology at the James P. Wilmot Cancer Center, University of Rochester Medical Center in New York. The Ryan Laboratory employs molecular biological, immunological and biochemical tech- niques and implements clinical dietary studies to explore enteric diseases, namely diarrhea and colon cancer, affecting people in both developing and developed countries. Studying mechanisms of pathogen infection and metabolic disturbances in animals and people pro- vides essential information that is leading to novel dietary strategies and molecular targets. Ryan’s research program focuses on the interaction of dietary compounds with the im- mune system and lymphoid tissues in the gut. Her studies in mice, dogs, pigs and humans have led to numerous publications detailing translational knowledge about the promising role for the agricultural byproduct rice bran to promote health. Her laboratory collaborates with researchers from Fort Collins, University of North Carolina, University of Illinois, Virginia Polytechnic Institute and State University, Michigan State University and University of Colo- rado. International research collaborations are established in Mali, Kenya, Nicaragua, Nepal, and India. Ryan has also distinguished herself in global public health and food security. Her work is funded through National Institutes of Health, National Cancer Institute; the Bill and Melinda Gates Foundation, University of Colorado Cancer Center, and the Dry Bean Health Research Group. 2 16TH ANNUAL RESEARCH DAY CVMBS RESEARCH DAY 2015 OUR 16TH ANNUAL RESEARCH DAY SCHEDULE of Events showcases the academic work of more than 140 aspiring scientists in 11:30–NOON POSTER SET UP SALON III, IV Colorado State University’s College of Veterinary NOON OPENING REMARKS SALON II Medicine and Biomedical Dr. Melinda Frye, Associate Dean for Veterinary Sciences. The day gives our Academic and Student Affairs rising stars vital experience 12:05 P.M. CVMBS-Zoetis Early Career Research Award Winner SALON II presenting their research Dr. Candace Mathiason “Stealthy Prions” findings to a scientific audience through poster 12:25 P.M. CVMBS-Zoetis Early Career Research Award Winner SALON II displays and talks. The Dr. Elizabeth Ryan “Rice Bran: Cross-Kingdom and day also provides young One Health perspectives” researches with an avenue for feedback to help them 1-5 P.M. ORAL PRESENTATION I: Clinical Sciences SALON I develop ideas that, in many 1-5 P.M. ORAL PRESENTATION II: Basic Sciences SALON II cases, will become lifelong scientific pursuits. In a 1-5 P.M. ORAL PRESENTATION III : Clinical/Basic Sciences SALON V sign of significance, the research projects on dis- 1-3 P.M. POSTER SESSION I JUDGING: SALON III, IV Odd-numbered Posters play are sponsored by two dozen well-respected com- 2:45 P.M. BREAK panies, foundations, and institutions concerned with 3:15-5 P.M. POSTER SESSION II JUDGING: SALON III, IV improving human, animal, Even-numbered Posters and environmental well- being. Thank you for 5-6 P.M. SOCIAL HOUR SALON III, IV supporting and engaging 6 P.M. AWARDS SALON III, IV with our presenters – undergraduate students, graduate students, veterinary residents, and post-doctoral fellows - as they pursue research that will help animals, people, and the planet! SCIENTIFIC PROCEEDINGS 3 ORAL PRESENTATIONS | CLINICAL SCIENCE SESSION 1: Clinical Science 1:00–5:00 PM | SALON I 1:00 Bartner Bartonella spp. PCR assay results using cerebrospinal fluid of naturally exposed dogs with central nervous system disease | CS 1:15 Bromberek Canine B-cell chronic lymphocytic leukemia shows strong breed-specific risk | MIP 1:30 Colbath Single Paralumbar Fossa Laparoscopy for Elective Ovariectomy in Standing Mares | CS 1:45 Coleman Investigation of gastrointestinal motility in dogs undergoing prophylactic laparoscopic gastropexy | CS 2:00 Fagre Improved characterization of Salmonella enterica shedding among reptile patients at the James L. Voss Veterinary Teaching Hospital | CS 2:15 Felumlee Intra- and interobserver variability of GFR rate determination in cats with chronic kidney disease via gamma camera uptake of Tc-99m-DTPA | ERHS 2:30 Fisher Carbon dioxide induced pulmonary hemorrhage | MIP 2:45 BREAK 3:00 Forster Navy bean powder diets modulate serum cholesterol and fecal lipid metabolites in healthy companion dogs | ERHS 3:15 Freund Assessment of novel digital and smartphone goniometers for joint angle measurement of the canine stifle | CS 3:30 Hart Comparison of owner satisfaction with stifle orthoses or tibial plateau leveling osteotomy for the treatment of cranial cruciate ligament disease | BMS 3:45 Hoaglund Effect of intravenous fluid volume administration on development of post-operative ileus in horses with colic | CS 4:00 Lakin The estrus cycle influences the bacterial microbiome in the normal mare reproductive tract | CS 4:15 Lantz Assessing pain and trauma in early lactation dairy cattle | CS 4:30 Lee Cationic liposomes-oligonucleotide complexes as an alternative adjuvant for polyclonal antibody production in rabbits | MIP 4:45 Martin Canine Gait Analysis Using Inertial Measurement Units During Outdoor and Treadmill Activity | CS DEPARTMENTAL ABBREVIATIONS BMS: Biomedical Sciences CS: Clinical Sciences ERHS: Environmental and Radiological Health Sciences MIP: Microbiology, Immunology, and Pathology 4 16TH ANNUAL RESEARCH DAY ORAL PRESENTATIONS | BASIC SCIENCE SESSION 2: Basic Science 1:00–5:00 PM | SALON II 1:00 Adney Efficient replication and shedding