21.10.2014SLAMF1 antibody (pAb) Rabbit Anti-Human/Mouse/Rat Signaling lymphocytic activation molecule family member 1 (CD150, CDw150, IPO -3, SLAM) Instruction Manual Catalog Number PK-AB718-6247 Synonyms SLAMF1 Antibody: Signaling lymphocytic activation molecule family member 1, CD150, CDw150, IPO-3, SLAM Description The signaling lymphocyte-activation molecule family member 1 (SLAMF1) is a novel receptor on T cells that potentiates T cell expansion in a CD28-independent manner. SLAMF1 is predominantly expressed by hematopoietic tissues. Reports suggest that the extracellular domain of SLAMF1 is the receptor for the measles virus and acts as a co-activator on both T and B cells. It is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations of the SLAM associated gene may be associated with X-linked lympho-proliferative disease (XLP). Quantity 100 µg Source / Host Rabbit Immunogen SLAMF1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SLAMF1. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Two isoforms of SLAMF1 are known to exist; this antibody will recognize both isoforms. SLAMF1 antibody is predicted to not cross-react with other SLAM protein family members. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, IHC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. SLAMF1 antibody can be used for detection of SLAMF1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. Images Available upon request. References Cocks BG, Chang CC, Carballido JM, et al. 1995. A novel receptor involved in T cell activation. Nature 1995; 376:260-3. Tatsuo H, Ono N, Tanaka K, et al. SLAM (CDw150) is a cellular receptor for measles virus. Nature 2000; 406:893-7. Aversa G, Chang CC, Carballido JM, et al. Engagement of the signaling lymphocytic activation molecule (SLAM) on activated T cells results in IL-2-independent, Cyclosporin A-sensitive T cell proliferation and IFN-alpha production. J. Immunol. 1997; 158:4036-44. Images Available upon request. Related Products Cat. No. PK-AB718-6247P - SLAMF1 Peptide Cat. No. PK-AB718-1472 - Rat Colon Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .