Abstract Book 6th Pan-American Dengue Research Network Meeting April 9-12 Galveston, TX, USA WELCOME TO THE 6TH PANDENGUE NET MEETING IN GALVESTON, TX! Thanks to all of you for participating in the 6th PanDengue Net meeting! It is an honor that The PanDengue Net executive committee selected Galveston and The University of Texas Medical Branch (UTMB) to host this meeting. The Gulf Coast of Texas and specifically Galveston has had a rich history of circulation of arboviruses, such as dengue and yellow fever, and development of vector control programs to prevent their impact on human public health. Since 1994, UTMB has built an infectious disease program that is internationally recognized as a leader in emerging and arboviral diseases. This meeting will give the opportunity to professionals who work in different areas of arboviral infections, such as dengue, chikungunya, Zika, Mayaro, yellow fever as well as other emerging and neglected arboviruses, to present their latest research and establish collaborations that will further enhance their visibility and scientific vistas. We also hope that this meeting will offer the opportunity to all trainees to not only network with established investigators but also to exchange ideas and share their vision about their work. Thanks to the members of the Executive and Scientific Committees and all volunteers for their effort and passion in the organization of this Meeting. We hope you will enjoy the meeting and our Texas hospitality! Nikos Vasilakis, PhD Mariano Garcia-Blanco, MD, PhD, Professor and Vice Chair for Research Mildred Hajek Vacek and John Roman Vacek Department of Pathology Distinguished Chair Center for Biodefense and Emerging Infectious Professor and Chair Diseases Department of Biochemistry and Molecular Center for Tropical Diseases Biology Institute for Human Infections and Immunity University of Texas Medical Branch University of Texas Medical Branch University of Texas Medical Branch Galveston TX USA Scott Weaver, PhD John Sealy Distinguished University Chair in Department of Biochemistry & Molecular Human Infections and Immunity; Biology; Professor of Emerging Infectious Director, Institute for Human Infections and Diseases at the Duke-NUS Graduate Medical Immunity; School in Singapore Scientific Director, Galveston National Laboratory, Pei-Yong Shi, PhD University of Texas Medical Branch Kempner Professor of Human Genetics Galveston TX USA Department of Biochemistry & Molecular Biology University of Texas Medical Branch Galveston TX USA Executive Organizing Committee Nikolaos Vasilakis, Eva Harris, University of Texas Medical Branch, USA Univ. of California Berkeley, USA Scott Weaver, George Dimopoulos, University of Texas Medical Branch, USA Johns Hopkins Bloomberg School of Public Health Mariano Garcia Blanco, Duke Univ. Medical Center, UTMB USA Mauricio Nogueira, Univ de Sao Jose do Rio Preto, Brazil Pei-Yong Shi, University of Texas Medical Branch, USA Catalina Alfonso, Instituto Colombiano de Medicina Tropical Andrea Gamarnik, Fundacion Instituto Leloir, Buenos Aires Carlos Sariol, Secretary, Pan Dengue Net University of Puerto Rico, Puerto Rico Scientific Committee Eva Harris (Chairperson) Diagnostics/Prognostics/Clinical Andrea Gamarnik (Chairperson) Pei-Yong Shi Albert Ko Virology/Pathogenesis/Antivirals Irene Bosch Andrea Gamarnik Ernesto Marques Mariano Garcia-Blanco Jaime Torres Rosa Maria del Angel Jorge Munoz Andrea da Pollan Pei-Yong Shi Vector Biology/Ecology/Vector Control Amy Morrison Immunology/vaccines George Dimopoulos Eva Harris Nikos Vasilikis Aravinda de Silva Luciano Moreira Ana Sesma Roberto Barrera Scott Weaver Sandra Lopez Steve Whitehead Epi/Phylogenetics/Modeling/Burden Mauricio Nogueira Isabel Rodriguez Betz Halloran Christine Carrington Tyler Sharp Mariana Leguia (Peru) AGENDA Monday 4/09/18 PM Tuesday 4/10/18 AM Tuesday 4/10/18 AM Moderators: Mauricio Nogueira/Isabel Rodriguez Expo A Moderators: Berlin Londono/ Amy Morrison Expo B Epi/Phylogenetics (concurrent) Vector Biology (concurrent) Connor McMeniman [8:30 - 8:50 + 5 Q&A] Identification of Nuno Faria [8:30 - 8:50 + 5 Q&A] Chemosensory Circuitry Orchestrating Aedes aegypti Attraction to Humans Myrna Bonaldo [8:55 - 9:15 + 5 Q&A] Genomic and structural features of Berlin Londono [8:55 - 9:15 + 5 Q&A] Mosquito saliva: Where are we the Yellow Fever virus from the ongoing Brazilian outbreak in terms of tracking vector-host interactions? Mauricio Nogueira [9:20 - 9:40 + 5 Q&A] The interplay of Zika and other Bradley White [9:20 - 9:40 + 5 Q&A] The Debug Project at Verily: Flavivirus in an endemic area Scaling the Sterile Insect Technique Short talk 1 Drumond, Betania Yellow fever epizootics in urban areas: Short talk - 1 Granada, YE [9:40 - 9:50 + 5 Q&A] Aedes Aegypti Brazil, 2016/2017 [9:40 - 9:50 + 5 Q&A] Resistance is associated with mutations in sodium channel Short talk 2 Cruz, Cristopher THE PHYLOGEOGRAPHY AND Short talk - 2 Hughes G [9:55 - 10:05 + 5 Q&A] Microbiota and Zika PHYLODYNAMICS OF DENV-3 IN PERU virus interactions in field-collected Aedes aegypti Registration Monday to Wednesday AM Coffee Break 10:10 - 10:30 Coffee Break 10:10 - 10:30 8:00 am-6:00 pm Moderators: Mauricio Nogueira/Isabel Rodriguez Moderators: Berlin Londono/ Amy Morrison Thursday 8:00 am- 12:00 pm Modeling/Burden (concurrent) Vector Ecology/Control (concurrent) Isabel Rodriguez-Barraquer [10:30 - 10:50 + 5 Q&A] Towards refining the Luciano Moreira [10:30 - 10:50 + 5 Q&A] City-wide deployment of global dengue map: insight from seroprevalence and case data Wolbachia in Brazil as an arbovirus control strategy Zulma Cucunuba [10:55 - 11:15 + 5 Q&A] Combining different information Pablo Manrique [10:55 - 11:15 + 5 Q&A] New Tools for the Control of sources to estimate the burden of Chikungunya epidemic, the case of Aedes aegypti and challenges for control programs Colombia Eugenia Corrales [11:20 - 11:40 + 5 Q&A] Neotropical Bats that Co-habit Julien F. Pompon [11:20 - 11:40 + 5 Q&A] How non-coding flaviviral with Humans Function as Dead-End Hosts for Dengue Virus. RNA enhances mosquito transmission Short talk 1 - Sequetin, Mariana - Yellow fever in São Paulo State: a Short talk - 1 Ortiz, M [11:45 - 11:55 + 5 Q&A] Integrated Vector threat in peri-urban centers? [11:45 - 11:55 + 5 Q&A] Control Management After Two Hurricanes in Puerto Rico Short talk 2- Coello-Escoto, Ana - A pipeline for measuring antigenic Short talk - 2 Astete, H .[12:00 - 12:10 + 5 Q&A] COMPARISON of the relationships among diverse, low-passage dengue viruses [12:00 - 12:10 + efficacy of the bg sentinel and suna traps for capture of Aedes aegypti 5 Q&A] mosquitoes in Peru Lunch: 12:20 am - 1:30 pm Moderators: Eva Harris and Ana Sesma Expo A Epi/Immunology DENV-ZIKV Leah Katzelnick [1:30 - 1:50 + 5 Q&A] Effect of pre-existing antibodies to DENV and ZIKV on subsequent DENV and ZIKV antibody titers, infection, and disease in Mauro Teixeira [1:55 - 2:15 + 5 Q&A] The role of glutamate on Zika-induced neuronal damage Alex Sette [2:20 - 2:40 + 5 Q&A] CD8 T cell immunity to Zika virus in humans is shaped by prior DENV exposure and associated with an IFN gamma/cytotoxic signature Short talk - 1: Delgado et al. Cross-reactive immune responses upon sequential dengue and Zika virus infection [2:45 - 2:55 + 5 Q&A] PM 6-9 Meet & Greet Short talk 2 Haller et al., MAYV emergence in a CHIKV endemic world: Is MAYV a new a threat? - 3:00 - 3:10 + 5 Q&A] Hotel Convention Center Expo Coffee Break 3:15 - 3:45 Moderators: Eva Harris and Ana Sesma Expo A 6:30 Call to order - Nikos Vasilakis Immunology 6:35 General welcome to Galveston and Ernesto Marques [3:45 - 4:05 + 5 Q&A] Zika and Dengue antibody interactions: implications for diagnostics and vaccines introduction to UTMB - Danny Jacobs 6:45 Acknowledgments, credits and last minute announcements to participants - Irene Ramos [4:10 - 4:30 + 5 Q&A] Immune profiling of dengue virus infections in human immune cells Nikos Vasilakis, Carlos Sariol and Andrea Gamarnik 7:00 Introduction of Keynote Speaker - Eng-Eong Ooi [4:35 - 4:55 + 5 Q&A] Insights into determinants of symptomatic flaviviral infection Robert Tesh 7:10 Keynote Lecture - Tom Monath “Yellow Fever: Creature from the Black Short talk - 1 Tan et al. -Guey Chuen Perng-, Functional analysis of PBMC in dengue endemic and non-endemic regions [5:00 - 5:10 + 5 Q&A] Lagoon” Short talk - 2 Ramjag et al., Characterization of Immune Responses to Selected Arboviruses and determination of seroprevalence in Trinidad 8:00 Reception [5:15 - 5:25 + 5 Q&A] Break [4 - 5 pm] POSTER SESSION 5 - 7 pm (Poster Session will run from Tuesday 8:00 am to Thursday noon) AGENDA Wednesday 4/11/18 AM Thursday 4/12/18 Moderators: Andrea Gamarnik/ MarianoGarcia-Blanco Expo A Moderators: Pei Yong Shi/ Albert Ko Expo A Virology Diagnostics/Prognostics Eva Harris [8:30 - 8:50 + 5 Q&A] Development and evaluation of Rosa Maria del Angel [8:30 - 8:50 + 5 Q&A] NS3 and NS5 from dengue and Zika modulate serological methods for Zika diagnosis and surveillance and use in nuclear citoplasmic transport and innate immune response by targeting nuclear proteins studies of seroprevalence and long-term immunity Stacy HORNER [8:55 - 9:15 + 5 Q&A] Defining how the RNA modification m6A regulates Irene Bosch [8:55 - 9:15 + 5 Q&A] A low cost methodology to detect Flaviviridae virus infection specific exposure to Zika virus Cybele Garcia [9:20 - 9:40 + 5 Q&A] Role of PML in the intrinsic immunity against Susan Wong [9:20 - 9:40 + 5 Q&A] The flavivirus multiplex microsphere flaviviruses immunoassay for recent