International Journal of Science and Research (IJSR) ISSN: 2319-7064 SJIF (2019): 7.583 Minerals and Phytochemical Analysis of Bark of Derris scandens (Roxb.) Benth Kishorjit Singh Maharabam1, Rameshor Singh Atom1, 2, Dini Ahanthem1, Warjeet Singh Laitonjam1* 1Department of Chemistry, Manipur University, Canchipur- 795003, Imphal, Manipur, India 2Waikhom Mani Girls’ College, Thoubal-795138, Manipur, India *Corresponding author E-mail: warjeet [at]manipuruniv.ac.in; warjeet [at]yahoo.com Abstract: The Himalaya mountain Range is hub of medicinal plants. The Derris scandens (Roxb) Benth is a plant offabaceae which has been used by indigenous people of Manipur, North-East India, for treatment of various purposes such as removal stones in kidney, loss of appetite, menstrual cycle in women, massage of body, solvent in laboratory, nourishment of child etc. It is commonly found in the forests of northern Oudh, in central and southern India, extending to Bengal, Assam, the Andaman and Nicobar Islands, Srilanka, Burma, southern China and North Australia (Duthie 1903–1929) The plant usually grows in the mountainous region of Manipur. The elemental analysis and phytochemical screening of the bark of the plant was carried out.Inductively coupled plasma -mass spectrometry and FAAS determined the presence of the elements Na ( 0.6µg/ml), K (10.9µg/ml), Fe (1.156µg/ml), Mg (2.2156µg/ml), Ca (13.0µg/ml), As (less than 5 ng/ml), V (less than 2 ng/ml), Cr (7 ng/ml), Mn (167 ng/ml), Ni (6 ng/ml), Co (less than 2ng/ml), Cu (31ng/ml), and Pb (12ng/ml). Phytochemical screening of the bark extracts of water, chloroform and petroleum ether confirmed that water extractcontain maximum phytochemicals viz. glycosides, saponin, alkaloid, terpenoid, steroid, phenol and tannin as compounds. The present finding will definitely helpful in the preparation of further pharmaceutical formulations. Keywords: Fabaceae, ICP-MS, FAAS, minerals, Phytochemicals, nourishment 1. Introduction pretreatment for cells before irradiation which synergistically sensitizes HT-29 cells to the radiation- The fabaceae or leguminous family also commonly known induced cell death by apoptosis or by mitototic catastrophe. as pea or legume or bean family is one of the important This plant extract are also used for treatment of silences pro- flowering plant due to its medicinal value. It consists of survival signaling [3]. 2) The compound isolated from derris varieties of trees and herb plants of perennial or annual and plant is used as insecticide and act as a feeding deterrent can be recognized by their fruits and stipulated leaves. against the pests for stored grains [2]. 3) The compound Presently, it is considered as one of the third largest plant obtained from this plant have also the capability for cytokine family after orchidaceae and asteraceae family based on its secretion [4].In this regard, our laboratory has focused on abundance and has about 730 genera and over 14000 isolating the medicinally important plant extracts and species. Some of the species of this family are found in the structurally define the major components. Further validate mountainous region and the valley of Manipur and are used the use of the extracts using common techniques. as food namely, glycine max (soybean), phaseolus (beans), pisumsativum (pea), cicerarietinum (chickpeas), medicago Based on our hypothesis, we proposed to extract and sativa (alfalfa), arachishypogaea (peanut), ceratoniasiliqua characterize the commonly practiced plants extracts from (carob), and glycyrrhizaglabraetc [1]. Many of the plants of Derris scandens. In Manipur, the product obtained from this this family are known for their medicinal properties and plant are used as1) removal of kidney stones. 2) against loss used in the natural preventive treatments of common of appetite. 3) treatment of menstrual cycle in women. 4) diseases like asthma, abscess, anthelmintic, astringent, massage of body. 5) solvent in laboratory. 6) nourishment of cough and cold, fever, paralysis, piles, diarrhea, worm, heart child etc. This information obtained through the survey of disease, eczema, dad, whooping cough, ulcers, snake-bite, local experts who used this plant. ring worm, diuretic, breast pain, bronchitis, dysentery, gonorrhea, leprosy, burning sensation, kidney disease, blood The phytochemical test of this plant extracts in water, pressure, malaria, syphilis, cholera, opthalmia, psoriasis, chloroform and petroleum ether found that water extract sciatica etc. contained maximum of glycosides, saponin, alkaloid, terpenoid, steroid, phenol and tannin compound. Some of the fabaceae family plants that are used for treatment of diseases. They are Derris scandens (Roxb) The determination of traces element in the plant derris plant Benth, Acacia nilitica (L.), Albizialebbek (L.), were performed using inductively plasma coupled-mass Albrusprecaborious L, Albiziaprocera (Roxb.) Benth, spectrometry and FAAS. The plant powdered contained K Bauhinia acuminate L., Cajanuscajan (L.), and Ca in major amount and Na, Fe, Mg, As, V, Cr, Mn, Ni, CaesalpiniabondusL, Mimosapudica L, Pisumsativum L, Cu, and Pb. are present in smaller amount. The presences of Tamarindusindica L, etc. [1] above compounds such as glycosides, saponin, alkaloid, terpenoid, steroid, phenol and tannin etc and the traces In literature, the derris scandens (Roxb) Benth, one of the elements have contribution to above uses. As far as my class of plant within fabaceae family is used for 1) Volume 10 Issue 1, January 2021 www.ijsr.net Licensed Under Creative Commons Attribution CC BY Paper ID: SR21118172633 DOI: 10.21275/SR21118172633 1038 International Journal of Science and Research (IJSR) ISSN: 2319-7064 SJIF (2019): 7.583 literature survey is concerned no one has performed determination of elements in this plant. 2. Material and Method 2.1 Description of plant sample Derris scandens plant is a tree climber usually grows near the river in mountainous region. It is a perennial and took some years to become mature plant. It flowers in May –June at the start of rainy and spring season. Stages of the plant sample are shown in Fig1, Fig 2. Figure 3: Plant’s bark Sample Figure 1: Plant Figure 4: Powdering process Figure 2: Flower 2.2 Collection of Sample About 20 kg the bark of the plant were collected from the Figure 5: Powdered sample mountainous region of Sapormeina, Kangpokpi District of Manipur in North East India, Fig 3. The collections of barks 2.4 Elemental Analysis were done in January to February. a) Sample preparation for analysis: The plant bark 2.3 Processing of Sample powder was measured around 1 gm and dissolved in 10mL of nitric acid followed by the volume was made a) Cleaning of sample: The bark of plant was thoroughly 100 mL by adding deionised water. This solution is washed with normal water to remove impurities and then preserved for ICP-MS and FAAS studies. The pellets of again washed with distilled water. Finally, plant bark was 1 cm diameter were prepared under hydraulic pressure washed with deionised water to remove impurities and these were send for SEM- EDAX study. present in the bark. b) Characterization methods: The determination of b) Drying of the extract: The bark of plant was dried in elements was done by Flame atomic absorption shed and open air at room temperature so that not to spectroscopy with model GBC 906AA AAS unit and affect chemical composition of the plant bark. The colour deuterium-arc background correction was employed. The of the bark is brown outside and light yellow inside. The elements Na, K, Fe, Mg, and Ca were determined up to bark was shed air dried for 3 to 4 weeks until the colour ppm (µg/ml). Nano-pure water (18.3 Mega ohms) as changes into brown. The dried plant bark was stored in diluent in this estimation. And the air-acetylene flame air tight container to avoid incorporation of unwanted was employed. The determination of elements was also impurity that might change chemical composition of the done by inductively coupled plasma mass spectrometer sample. with model VG PQ ExCell, VG Elemental, UK. The c) Powdering of the extract: The dried plant bark was elements such as Cr, Mn, Co, Cu, Pb, As, V were powdered using electric grinder. The powder plant bark determined and reported up to ppb (ng/ml), The Relative was stored in glass container for analysis, Fig 4, Fig 5. standard deviation was measured by using the following formula Relative standard deviation (RSD) = (SD 100)/x, where x is provided in ppm or ppb as mean. The Relative standard deviation values for Flame atomic Volume 10 Issue 1, January 2021 www.ijsr.net Licensed Under Creative Commons Attribution CC BY Paper ID: SR21118172633 DOI: 10.21275/SR21118172633 1039 International Journal of Science and Research (IJSR) ISSN: 2319-7064 SJIF (2019): 7.583 absorption spectroscopy and inductively coupled plasma Lead acetate test: A few drops of lead acetate solution are mass spectroscopy techniques are calculated to be 2-5 % treated with the extract. The yellow colour solution and 5-10%, respectively. formation shows the presence of flavonoids. 4) Detection of phenols 2.5 Phytochemical Screening To the plant extract a few drops of ferric chloride solution was added. The formation of bluish black colour shows a) Preparation of different extracts the presence of phenols. The bark powdered of plant, Derrisscandens (Roxb.) 5) Tannins test: Benth was soaked in water for 5 days and the water The extract is mixed with a few drops of 1 % gelatin soluble extracts was removed through filtration. The solution containing sodium chloride. The white water soluble extract was concentrated by Rotavapor and precipitate formation shows the presence of tannins. dried it at room temperature to get solid form. The 10 ml of bromine water was mixed with 0.5 g The soluble extract obtained through filtration was then aqueous extract. The decolouration of bromine water mixed with petroleum ether in separating funnel and two indicates the presence of tannins.