US0093.21701 B2 (12) United States Patent (10) Patent No.: US 9,321,701 B2 Burk et al. (45) Date of Patent: Apr. 26, 2016 (54) MICROORGANISMS AND METHODS FOR 2003/0233218 A1 12/2003 Schilling THE BIOSYNTHESIS OF BUTADENE 38885 A. 58: ManaSSO ca.al 2004/OO72723 A1 4/2004 Palsson et al. (75) Inventors: Mark J. Burk, San Diego, CA (US); 2009/0047719 A1 2/2009 Burgard et al. Anthony P. Burgard, Bellefonte, PA 2010.0003716 A1 1/2010 Cervin et al. (US); Jun Sun, San Diego, CA (US); 2010/0330635 Al 12/2010 Burgard et al. Robin E. Osterhout, San Diego, CA 392 A: i: Ewar 3. S. PritiD4- Pharkya, San Diego, CA 2014/O155567 A1 6, 2014 BurklKC et al. (73) Assignee: Genomatica, Inc., San Diego, CA (US) FOREIGN PATENT DOCUMENTS - GB 5251.98 8, 1940 (*) Notice: Subject to any disclaimer, the term of this WO WO O2/O55995 T 2002 patent is extended or adjusted under 35 WO WOO3/106998 12/2003 U.S.C. 154(b) by 0 days. WO WO 2007 141208 12/2007 WO WO 2008/115840 9, 2008 WO WO 2010.006O76 1, 2010 (21) Appl. 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