Published with support from the Commission on Higher Education (CHEd) Acta Manilana Volume 64 (2016) ISSN: 0065–1370 Official Publication of the Research Center for the Natural and Applied Sciences UNIVERSITY OF SANTO TOMAS ACTA Manilana Volume 63 (2015) ISSN: 0065–1370 EDITOR-IN-CHIEF Fortunato B. Sevilla III, Ph.D. MANAGING EDITOR Mafel C. Ysrael, Ph.D. SECTION EDITORS Christina A. Binag, Ph.D. (Chemistry) John Donnie A. Ramos, Ph.D. (Biology) Ma. Carlota B. Decena, Ph.D. (Mathematics) Marilyn C. Mabini, Ph.D. (Engineering) EDITORIAL ASSISTANT Christopher D. Purugganan INTERNATIONAL ADVISORY BOARD Salvador S. Alegret Ramaier Narayanaswamy Universitat Autonoma de Barcelona, Spain The University of Manchester, United Kingdom Geoffrey A. Cordell Cheong Nge University of Illinois at Chicago, U.S.A. Agency for Science, Technology and Research, Singapore Kevin D. Croft Takashi Okamoto University of Western Australia, Australia Nagoya City University, Japan Henri J. Dumont Stephen G. Pyne Ghent University, Belgium University of Wollongong, Australia Scott G. Franzblau Roger W. Read University of Illinois at Chicago, U.S.A. University of New South Wales, Australia Mary J. Garson Giorgio Sberveglieri The University of Queensland, Australia University of Brescia, Italy Hasuck Kim Steven L. Stephenson Daegu Gyeongbuk Institute of Science and Technology, University of Arkansas, U.S.A. Republic of Korea David Mark Taylor Nordin Hj. Lajis National University of Singapore, Singapore Universiti Putra Malaysia, Malaysia Shueh-Lin Yau Arben Merkoci National Central University, Taiwan, R.O.C. Catalan Institute of Nanoscience and Nanotechnology, Spain The ACTA MANILANA is a peer-reviewed journal published by the University of Santo Tomas for the Research Center for the Natural and Applied Sciences. All manuscripts, contributions or enquiries should be addressed to the ACTA MANILANA, Research Center for the Natural and Applied Sciences, University of Santo Tomas, España Boulevard, 1015 Manila, THE PHILIPPINES. Copyright 2015 by the University of Santo Tomas, Manila, Philippines. All rights reserved. SINGLE COPYING OF THE SINGLE ARTICLE FOR PRIVATE STUDY OR RESEARCH, IRRESPECTIVE OF WHERE THE COPYING IS DONE, IS PERMITTED. Multiple copying of the contents or parts thereof without permission is in breach of copyright . ACTA Manilana Volume 64 (2016) ISSN: 0065–1370 GRAPHICAL ABSTRACTS Molecular confirmation and taxonomy of the Rubiaceous Mycetia 1–7 Article apoensis (Elmer) Govaerts John Christopher C. Villanueva, Remigio S. Callanta Jr., Jasmin Aei F. Neptuno, Maryneil A. Verin, Porferio S. Bangcaya, Vincent Louie D. Cabelin, & Grecebio Jonathan D. Alejandro GC-MS metabolite profiling of the hexane extract and antimicrobial 8–16 Article characterization of the Philippine endemic Rubiaceae species Uncaria cordata var. circa, Psychotria luzoniensis, and Psydrax puberula Sarleen G. Castro, John Emmanuel V. Cid, Winnie Adrianne S. Ibañez, Grecebio Jonathan D. Alejandro, & Mario A. Tan https://www.slideshare.net/lourensrobberts/antimicrobial-susceptibility-testing-2013 17–23 Comparison of cellular-based viability and apoptosis assays on Article doxorubicin treated colorectal adenocarcinoma cells Thomas Adrian Tiongson, Maria Charina Magsumbol, Mark Kevin Devanadera, & Myla R. Santiago 25–32 Fluorophore-labeled bioengineered glucose binding protein Article for measurement of transdermal glucose Cristina Tiangco, Sheniqua Brown, Fortunato Sevilla III, Govind Rao, & Leah Tolosa 33–39 Enantioselective chiral 2-pyridyl-2-imidazoline organocatalyzed Aldol Article reactions in brine Aileen Marice R. Baltazar, Simon Budde, Christian Faderl, Aldrick B. Verano, & Allan Patrick G. Macabeo Cl- Na+ N N N - Na+ N N Cl H H OH O Na+ 1 1 R CHO O R - + Cl R2 R2 Na+ + - + - - Na Cl Na Cl Cl 67–74 A review of recent literature on repairable-item inventory systems Article Marilyn C. Mabini REPAIR SUPPLIER FACILITY REPAIRABLE-ITEM INVENTORY MODELS Single- Multi- WAREHOUSE Echelon Echelon scrap Deterministic Stochastic Deterministic Stochastic • Inventory policy: • Inventory policy: • Inventory policy: • Inventory policy: USERS fixed lot size (s-1,s); (Q.r): fixed lot size (s-1,s); (Q.r); (s,S): service level base (s-1,s), based depot (s,S) 75–86 Reviving the Philippine shrimp industry: molecular diagnostics Article and therapeutics Maria Violeta R. Tare, Pocholo Mari T. Arabit, Mark Anthony G. Fran, David Angelo V. Guanzon, Jalizah Jaira E. Lim, Sharlaine Joi Ann B. Orense, Joselito A. Tabardillo Jr., & Mary Beth B. Maningas 87–98 Natural products-based discovery of antitubercular agents from Philippine Article medicinal plants — A review Allan Patrick G. Macabeo, Oliver B. Villaflores, Scott G. Franzblau, & Ma. Alicia M. Aguinaldo N MeO H OMe N O O HO Ten plant families H O N H MeO H O Explored seventeen plants O O OH OMe Identified sixty compounds N OMe H O OMe OMe O OH MeO N N O HO O >5 promising antitubercular hit compounds!!! Acta Manilana Volume 64 (2016) ISSN: 0065–1370 Manila, Philippines ACTA Manilana GUIDE TO CONTRIBUTORS The ACTA MANILANA publishes papers dealing with any aspect of the natural sciences. Research papers, preliminary and short communications, and review papers are welcome. Papers submitted are considered with the understanding that they have not been published and are not under consideration for publication elsewhere. Every paper will be reviewed by at least two referees on whose recommendations the Editor’s decision on acceptance will be based. Script Requirement Two complete copies of the manuscript are required. They should be clearly typed double- spaced on an 8.5 11 inches paper. Original photograph must be included when applicable. An e-copy of the manuscript (using any of the following program: MSWord or Adobe PageMaker) should also be submitted. Please indicate the e-mail addresses of the corresponding author(s). Each manuscript should include the following: (1) Title page, which should contain the title of the paper, the names of the authors, and the institutions where the work has been carried out. The title should be concise and informative with a high keyword content. The names of its authors should include first name, middle initial, and surname. If the author has left the institution mentioned, his present address should be given as a footnote.The corresponding author’s full mailing address, plus phone and fax numbers and email address should be included. The author to whom correspondence should be addressed should be indicated with an asterisk (*). (2) Abstract, which should comprise a factual account of the contents of the paper with emphasis on what is new and/or original in the paper. It must be concise (up to 200 words) summary of the whole paper. (3) Keywords, which should be not more than six. (4) Introduction, which should mention in a concise manner previously published work and start clearly what is new in the paper now submitted. (5) Experimental, which should describe the methods and materials used with sufficient detail to enable any reasonably experienced workers to carry out the procedure. Description of well- known techniques and equipments are not necessary. In writing, complete sentences should be used, and consistency in voice and tense should be observed. (6) Results and discussion, which should be complete and relevant but concise. Verbose exposition of ideas should be avoided, and unsupported elaboration of hypothesis should not be included. (7) Acknowledgment, which should include credits for financial support, technical assistance and sources of special materials. Professional titles and wordy sentences should be avoided. (8) References, which are listed on a separate page at the end of the manuscript. The references are numbered consecutively in the text where they are indicated by an Arabic numeral enclosed in square brackets. The following format should be used in the listing: [1] Calixto NO, Pinto MEF, Ramalho SD, Burger MCM, Bobey AF, Young MCM, Bolzani VS, & Pinto AC. The Genus Psychotria: Phytochemistry, Chemotaxonomy, Ethnopharmacology and Biological Properties. Journal of the Brazilian Chemical Society 2016; 27(8):1355–1378 [2] Caipang C. Utilization of loop-mediated isothermal amplification (LAMP) for the detection of pathogens in shrimp and fish aquaculture in the Philippines. In: Caipang MM, CMA (Ed.) Biotechnological advances in shrimp health managament in the Philippines, pp. 149–163. (Kerala, India: Research Signpost, 2015). (9) Tables, which should be presented on separate sheets. They should be clearly arranged, provided with appropriate captions and numbered consecutively. (10) Illustrations and figures, which should be used as sparingly as possible. Original drawings of graphs and diagrams should be submitted on plain white paper or in tracing paper. Glossy prints of photographs should be supplied. (11) Figure and table captions, which should be listed on separate sheets. The captions should be as brief as possible. Acta Manilana 64 (2016), pp. 1–7 Printed in the Philippines ISSN: 0065–1370 Molecular confirmation and taxonomy of the Rubiaceous Mycetia apoensis (Elmer) Govaerts John Christopher C. Villanueva1, Remigio S. Callanta Jr.1, Jasmin Aei F. Neptuno1, Maryneil A. Verin1, Porferio S. Bangcaya3, Vincent Louie D. Cabelin4, & Grecebio Jonathan D. Alejandro*1,2 1Department of Biological Sciences, College of Science; 2Research Center for the Natural & Applied Sciences, University of Santo Tomas, 1015 Manila, Philippines; 3College of Teacher Education, Biological Science Department,