PRINCIPLES OF REPRODUCTIVE BIOLOGY COURSE SYLLABUS Course Name: Principles of Reproductive Biology Course Number: PSL 7690 Course Directors: Jeyasuria Pancharatnam, PhD, [email protected], Office: 313-577-2153 Course Location: CS Mott Center for Human Growth and Development, Department of Obstetrics and Gynecology, 275 E. Hancock between John R and Brush streets Credit Hours: Three (3) Course Offered: Fall Semester, September 1 to December 16, 2020 – Wednesdays and Fridays (15 weeks x 2 lectures or Journal clubs/week) Format: Lecture – Two sessions per week, 1 hour/session, 10:00 am – 11:00 am, CS Mott Center Conference Room 135.1 Course Description: Covers principles and techniques in reproduction including: endocrinology; gametogenesis; fertilization; implantation; embryogenesis; stem cell determination; pregnancy; and parturition. Some knowledge of biology, genetics, embryology, and molecular biology recommended. Learning Objectives/Outcomes: Students will understand how to use the scientific method and analyze recent and classic literature and techniques to design experiments that solve cutting edge hypotheses in reproductive and developmental sciences. Students will be expected to read the literature in reproductive physiology and present a seminar/journal club presentation of the material. Assessment Method: 1 Midterm and Final, Journal Club MIDTERM – 10/21/19 35% of final grade FINAL – 12/9/19 45% of final grade JOURNAL CLUB 20% of final grade Other Events: Thanksgiving Holiday Break, November 26-29, 2019 Reference Book: Essential Reproduction, 8th Edition, Martin H. Johnson Course Faculty: Office hours are by appointment. Faculty Office Phone Email Ayesha Alvero, MD CS Mott 577-1794 [email protected] D. Randall Armant, PhD CS Mott 577-1748 [email protected] Tracie Baker, DVM, PhD I-Bio 577-2462 [email protected] Kang Chen, PhD Elliman 578-4339 [email protected] Jennifer Condon, PhD CS Mott 577-2152 [email protected] Nardhy Gomez-Lopez, PhD CS Mott 577-8904 [email protected] John Hannigan, PhD MPSI 664-2503 [email protected] Stephen Krawetz, PhD CS Mott 577-0765 [email protected] Gil Mor, MD, PhD CS Mott 577-1337 [email protected] Nihar Nayak, DVM, PhD CS Mott 577-8910 [email protected] Jeyasuria Pancharatnam, PhD CS Mott 577-2153 [email protected] Daniel Rappolee, PhD CS Mott 577-1228 [email protected] Douglas Ruden, PhD CS Mott 577-6688 [email protected] 1 Sana Salih, MD CS Mott 248-352-8200 [email protected] Zhibing Zhang, MD, PhD CS Mott 577-0442 [email protected] 1. Introduction ((Jeyasuria) (09/02) An overview of sexual reproduction. Why? The Germ cell-Zygote- implantation and pregnancy. 2. Adult Male Anatomy and Reproductive Physiology (Jeyasuria) (09/04) Description of male anatomy Testis divided into somatic vs germ cells and their reproductive function Leydig cell function and steroidogenesis Sertoli cell function and germ cell maturation HPG axis function, the onset of puberty Sperm transport-vas deferens, prostate, etc. 3. Spermatogenesis-Spermatogenic Stem Cells to Mature Sperm (Jeyasuria) (09/09) Meiosis vs mitosis, spermatogenesis, spermiogenesis Reshuffling of paternal and maternal genes through chromosomal crossover and segregation Clinical aspects – chromosomal abnormalities 4. Adult Female Anatomy and Reproductive Physiology (Condon) (09/11) The anatomy and function of uterus, cervix, vagina, fallopian tubes and breast Granulosa cells and thecal cells – Steroidogenesis: the two-cell model 5. Oogenesis I (Jeyasuria) (09/16) Transcriptional regulation of PGC specification Germ cell development – Germ cell numbers Meiosis – Regulation of cell cycle arrest Male/Female differences in gametogenesis 6. Oogenesis II (Salih) (09/18) The onset of puberty The HPG axis and control of the menstrual cycle Oocyte maturation – Primary follicle to graffian follicle Hormonal control of folliculogenesis and ovulation Clinical aspects – Infertility 7. Fertilization and Activation of Development (Zhang) (09/23) Sperm-egg recognition Acrosome reaction and species barriers 8. Journal Club (09/25) 2 9. Preimplantation (Rappolee) (09/26) Embryo transport Zygote gene activation Cleavage stages Inner cell mass (ICM) and trophectoderm Lineage specification 10. Implantation (Armant or TBA) (10/02) Morphological and molecular aspects of blastocyst programing for implantation Trophoblast adhesion and differentiation during implantation Coordinate interactions of trophoblast and endometrium Hormonal control of uterine receptivity 11. Experimental Embryology (Ruden) (10/07) Maternally and paternal imprinting (Igf2 and H9) X-chromosome inactivation Imprinting and cloning Genomic imprinting and human disease Epigenetics (definition) Chromatin remodeling Methylation and CpG islands, histone methylation 12. Placental Development and Function (Nayak) (10/09) Introduction – evolutionary perspective Placentation – classification Placental structure – comparative biology – emphasis on primates and rodents Decidua, fetal membranes, umbilical cord, amniotic fluid Placental circulation – primates and rodents Placental function – metabolism, transport and endocrine Animal models for studying placental development and function 13. Pregnancy (Condon) (10/14) Growth and differentiation of the pregnant uterus Overview from implantation to term Regulation of gestational length Origins and actions of estrogen and progesterone Modulators of uterine progesterone receptor action 14. Journal Club (10/16) MIDTERM EXAM (10/21) 15. An introduction to reproductive immunology(Mor/Alvero) (10/23) Concepts Immune cell interactions Humoral immunity vs ---- 3 16. General Concepts of Maternal Fetal Immune Interaction (Mor) (10/28) The process of maternal/fetal immune acceptance Immune tolerance, mechanisms Role of the immune system during pregnancy Antigen presentation during pregnancy Modulation of immune responses by the endometrial microenvironment 17. Immune Cells and Mediators in Pregnancy (Chen) (10/30) Male and female reproductive immune systems Immune modulatory functions of the seminal fluid Immunity at the maternal-fetal interface in early pregnancy Immune cells, mediators and pathways that maintain fetal tolerance Immune functions of the amniotic fluid Maternal-fetal immunity in late pregnancy and parturition Transfer of maternal passive immunity to the fetus SPECIAL TOPICS IN REPRODUCTION — 18. Immune Mediators that Lead to Term and Preterm Labor (Gomez-Lopez) (11/04) Inflammation Infection Immunology 19. Reproductive Toxicology (Baker) (11/06) Endocrine disrupting compounds (EDCs) Developmental origins of health and disease Transgenerational effects Model organisms for evaluation and testing 20. IVF/ART (Salih) (11/11) Acquire skills to evaluate infertility Review of hormones that regulate the ovarian cycle Learn the hormones used for controlled ovarian hyperstimulation Describe the process of in vitro fertilization (IVF) Assessment of embryo growth in the laboratory Review the impact of IVF on maternal and child health Understand the regulatory processes that apply to IVF 21. Towards the Birth of a Healthy Child: The Male Contribution in the Era of Precision Medicine (Krawetz) (11/13) The System – Contribution - Task Discovery and changing paradigms Developing molecular diagnostics A working model Translation to the clinic 4 22. Journal Club (11/18) 23. Teratology (Hannigan) (11/20) The emergence of modern teratology Principles of Teratology ➢ Mechanisms and models ➢ “Epigenesis” versus “Epigenetics” The “varieties of teratogenic experience” ➢ Selected examples (dietary, biological, environmental, pharmacological) Prevention and intervention: Counseling and screening 24. Reproductive Cancers (Alvero) (11/27) Emphasis will be on female reproductive cancers with focus on ovarian cancer Clinical profiles will be discussed with the goal of identifying clinical problems that should be addressed by current research Relevant pro-tumor pathways (i.e. stem cell and cell death pathways) will also be discussed with the goal of highlighting unresolved issues that can be answered by basic science research 26. Journal Club (12/02) 27. Review of Material (12/04) FINAL EXAM (12/09) UNIVERSITY POLICIES Religious holidays (from the online Academic Calendar): Because of the extraordinary variety of religious affiliations of the University student body and staff, the Academic Calendar makes no provisions for religious holidays. However, it is University policy to respect the faith and religious obligations of the individual. Students with classes or examinations that conflict with their religious observances are expected to notify their instructors well in advance so that mutually agreeable alternatives may be worked out. Student Disabilities Services: If you have a documented disability that requires accommodations, you will need to register with Student Disability Services for coordination of your academic accommodations. The Student Disability Services (SDS) office is located at 1600 David Adamany Undergraduate Library in the Student Academic Success Services department. The SDS telephone number is 313-577-1851 or 313-202-4216 for videophone use. Once you have your accommodations in place, I will be glad to meet with you privately during my office hours to discuss your special needs. Student Disability Services’ mission is to assist the university in creating an accessible community where students with disabilities have an equal opportunity to fully participate in their educational experience at Wayne State University.