See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/235874990 Phylogeny of Hepatica (Ranunculaceae) and origin of Hepatica maxima Nakai endemic to Ullung Island, Korea Article in Biological Journal of the Linnean Society · January 2011 CITATIONS READS 24 774 9 authors, including: Martin Pfosser Tod Stuessy Biocenter Linz University of Vienna 99 PUBLICATIONS 1,910 CITATIONS 192 PUBLICATIONS 6,258 CITATIONS SEE PROFILE SEE PROFILE Chang-gee Jang Yanping Guo Kongju National University Beijing Normal University 77 PUBLICATIONS 580 CITATIONS 50 PUBLICATIONS 846 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: to investigate for DMFC View project Central Asia Green Road Project View project All content following this page was uploaded by Martin Pfosser on 23 May 2014. The user has requested enhancement of the downloaded file. © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.at PFOSSER 'L!Hepatica maxima STAPFIA 95 (2011): 16–27 Phylogeny of Hepatica (Ranunculaceae) and origin of Hepatica maxima NAKAI endemic to Ullung Island, Korea M. PFOSSER*, B.-Y. SUN, T. F. STUESSY, CH.-G. JANG, Y.-P. GUO, K. TAEJIN, K. C. HWAN, H. KATO, & T. SUGAWARA Abstract: Nuclear ITS, chloroplast atpB-rbcL spacer and trnL-F intron and spacer sequences, as well as [<C[QP"<<< Hepatica¤YCRP endemic H. maxima[T[<<<Hepatica 'Anemone C`P"<'HepaticaX'"< CPtrnL-F #"6+<H. asiatica, H. henryi, H. insularis, and H. maxima#H. asiatica and H. insularis <'[< H. maximaH. asiaticaR' !R[Q[H. maxima'H. asiatica R'<^CH. nobilisP 'H. asiatica and H. insularis'<[Q' <<<< < Zusammenfassung7Y!"atpB-rbcL-IGS und trnL-F&YY:!&!" '[<C[QPT'< <:Hepatica[H. maxima, einem Endemiten Y¤Y"'T[&!&= =<:Hepatica<'[ :Anemone'HepaticaC`P" T:Hepatica['trnL-F !<Y=&@[T '6+<H. asiatica, H. henryi, H. insularis und H. maxima H. asiatica und H. insularis X<" [TT[ H. maximaH. asiatica- R'![Q&T 'H. maxima-H. asiatica&Q '' CH. nobilisP RT'H. asiatica und H. insularis- [Q&T'[' T&X<'@ &! Key words7 [Q <" Hepatica" " Y! " T[ " plastid microsatellites, speciation. *7 16 STAPFIA: reports © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.at PFOSSER 'L!Hepatica maxima STAPFIA 95 (2011): 16–27 A H. nobilis H. asiatica H. transsilvanica H. insularis H. maxima H. americana H. falconeri H. acutiloba H. henryi H. nobilis var. japonica var. pubescens B Manchuria A11-12 East Sea Korea A10 A9 Ullung Island A8 M1-7 A7 Japan A4-6 A1-2 A3 P1-3 J3 J1-2 I11 I12 I15 P4-5 I14 I13 I1-I10 200 km Fig. 1]UK!Hepatica'QU^p!N" H. asiatica (A1-12), H. insularis$::D;UH. maxima (M1-7), H. nobilisI'japonica (J1-3), H. nobilis I'pubescens$A:D;' Introduction @< < <" X'- Y < < YCBALDWIN & SANDERSON6**BARRIER6***P" 7 < - ^QYCSTUESSY & ONO6**P"<Y '["'< CFRANCISCO-ORTEGA 4556 RAY 6**P" = ""< CFRANCISCO-ORTEGA6***VARGAS6***P << <'- CEMERSON4554GRANT6**STUESSY & ONO6** WHITTAKER6**P! R¤&CPFOSSER4554P< <<CCRAWFORD & - STUESSY6**`P- <' " ' & < CSUN & STUESSY6**P STAPFIA: reports 17 © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.at PFOSSER 'L!Hepatica maxima STAPFIA 95 (2011): 16–27 ¤&6` < R55'^CQ6P"- AnemoneCEHRENDORFER455*EHREN- `4`)`)65+`)65_) DORFER & SAMUEL4556HOOT6**+MEYER4565 `2 SCHUETTPELZ4554P<<- *+ strate that Hepatica<Anemone that x`"< CKIM 6*" P & [¤ [ 4` - Island endemic H. maxima. <"' <Y!" <' plastid trnL-F intron and spacer, and atpB-rbcL spacer sequences K 6<C IM6*P[<- <<Hepatica worldwide. To "'"<[ <" <¤&"- <[[ R'^ <C[QP YANG <®<C 6**_P< <'¤Y - ^R [`55¤Y Materials and Methods `"4+ CLEE & YANG6*6SUN & STUESSY6**P- Taxa Sampled<Hepatica '¤Y R"¤&CRP" Y RC*+P"^C_P= CRP""["¤!["^6***&4556 C* CKIM 6*PP Q [CH. transsilvanica, H. henryiP- < #:P 'Acer, A. okamotoa- " " num and A. takesimense"<'CPFOSSER et =#[- 4554PA. mono and A. ¤' pseudosieboldianum"<"R [¤Y"H. maxima" DNA ExtractionT[' &' DOYLE DOYLE Hepatica MILLERC@P & C6*`P'['- CMELCHIOR6*_+P"CTAMURA6**P"CWEISS '`553[#C4 4554P""- [#"655="6+="45=T["54- " [ "X5P5_5553 [CQ6P- <C4+6P'' [['' 6+["T[ ¤H. maxima. NAKAICNA- ''553'' KAI6*`NAKAI6*4PR' '`56553 "H. asiatica and H. insularis' DNA Sequencing & - ¤Y"H. maxima,'' 'trnL(UAA) intron and the H. nobilis"japonicapu- CY:!P ' trnL(UAA)-) bescens^< the trnF(GAA)'[@ CHIROE6*`P# H. henryiH. CPFOSSER & SPETA 6***P atpB-rbcL transsilvanica CP [ '[4MANEN et al. [(H. acutiloba and H. americana; C6**+PY!'[' STEYERMARK & STEYERMARK6*_5P) and the threatened species Y!6&6 C)&M)P Y!+ C)&- H. falconeri'R M)P[[&T[' ''Hepatica, "' <[#Y``C 'CJALAS & SUOMINEN "¤RP'T<- 6**P[ H. maxima' C["¤![P#T< "<"- <!@<@RC[#<" "H. asiatica- ^P"CPFOSSER & SPETA RCSUN & STUESSY6**P'"R 6***P [ ' - endemic, H. insularis Y Y!4 C)&&)P Y! R" < < C)&&)P' related to H. asiatica CSUN & STUESSY6**WOO4554P Y!"6 #- ' H. asiaticaCLEE6*`_PARK6*`+P < Phylogenetic Analysis!' Hepatica['- T[6555[+55< 18 STAPFIA: reports © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.at PFOSSER 'L!Hepatica maxima STAPFIA 95 (2011): 16–27 57 Hepatica asiatica A13 Hepatica henryi 1 Hepatica insularis I16 H. nobilis var. japonica J4 H. nobilis var. pubescens P1 Hepatica acutiloba 3 Hepatica americana Hepatica nobilis 8 98 Hepatica transsilvanica 4 99 Anemone narcissiflora 2 100 Anemone narcissiflora 3 Anemone obtusiloba 100 Anemone flaccida 70 Anemone keiskeana 93 100 Anemone antucensis Anemone tenuicaulis 1 Anemone canadensis 100 Clematis afoliata 74 Clematis hexapetala Knowltonia vesicatoria 63 Pulsatilla patens 100 Pulsatilla montana 97 67 Pulsatilla koreana Pulsatilla occidentalis Anemone raddeana 100 66 Anemone nemorosa 1 100 Anemone sylvestris 1 74 Anemone blanda 1 Anemone drummondii Anemone rivularis 1 Ranunculus ficaria 98 Trautvetteria carolinensis 100 Trautvetteria grandis Trautvetteria japonica 5 changes Fig. 2Y"He- patica'!Anemone, Clematis, PulsatillaUKnowltonia' !TrautvetteriaRanunculus'QI$:/U///;!D/ I'!'I! x = %x = _!]- !!'p!H. maxima' T¤+5TT['& +5+CSWOFFORD4555P=' ¤::'C:& < 6"555 - :6**+P- &C#@P'Q- QT[' CFITCH6*`6P!'' < '' < < [=# '& C[6P<<- CFELSENSTEIN6*P'[ <C=P& C^P Y CP ''[¤ the trnL-F'" STAPFIA: reports 19 © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.at PFOSSER 'L!Hepatica maxima STAPFIA 95 (2011): 16–27 66 Hepatica asiatica A1a Hepatica henryi 1 Hepatica insularis I2 Hepatica americana 100 Hepatica acutiloba 2 Hepatica nobilis 3 Hepatica transsilvanica 1 64 H. nobilis var. japonica J1 H. nobilis var. pubescens P1a 74 96 Anemone narcissiflora 2 99 Anemone narcissiflora 1 55 Anemone obtusiloba 100 Anemone keiskeana 90 82 Anemone flaccida 92 Anemone tenuicaulis 2 97 Anemone antucensis 87 Anemone tenuicaulis 1 Anemone canadensis 1 Clematis hexapetala 62 Anemone x intermedia 78 Anemone sylvestris 2 73 Anemone coronaria Anemone ranunculoides Anemone nemorosa 2 61 98 Anemone pavonina Anemone hortensis 97 Anemone blanda 2 97 Anemone blanda 1 Anemone palmata 61 Anemone drummondii 92 Anemone rivularis 2 54 94 Anemone rivularis 1 Anemone hupehensis 78 98 Pulsatilla occidentalis Pulsatilla grandis Knowltonia vesicatoria Ranunculus ficaria 5 changes Fig. 3YatpB-rbcL- Hepatica'!Anemone, Clematis, PulsatillaUKnowltonia' ['QI$:/U///;!D/ I'!'I! x = %x = _!]!- !'p!H. maxima' C' AFLP Analysis.[Q<' P<'- ' [Q < C " Q "¤RP'[455T[' C<&<&[P'trnL-F ' < Eco@Y 66*&64*" _+&_*4" `_5& MseY3[C3P' ``6" 654_&65+ ' ' ' ' `[53T['& ' [T'<CX#[YTP45 =5_CMADDISON & MADDISON6**4P <45*+"+5_"5`4 -/ STAPFIA: reports © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.at PFOSSER 'L!Hepatica maxima STAPFIA 95 (2011): 16–27 H. acutiloba 1-2 H. asiatica A3, A10-12 H. insularis I1-4 H. transsilvanica 1-3 H. insularis I5-I15 67 H. asiatica A1-2, A4-6, A8 H. henryi 1-4 93 H. asiatica A7, A9 H. nobilis var. japonica J1-3 63 H. nobilis var. pubescens P1-5 H. nobilis 1-7 100 Anemone narcissiflora 1 Anemone canadensis 2 Clematis afoliata 95 Anemone palmata Anemone hortensis Anemone coronaria Fig. 4 " Y trnL-F - 'Anemone'x = _'Q I$:/U///;!D/I'p!H. maxima ' @'65& Results [ ' Eco@Y MseI Q&Eco@Y'- Nuclear ITS sequences [Q6_ " @ ' <C"- <"P Y! ' +6+ CY!67 4_5 Y!47 6+P 7 Eco&[[Mse&[" Eco&[:Mse&:" <&'< Eco&[[:Mse&!['- (Anemone, Clematis, Hepatica, Knowlto- '<['45*+" nia, Pulsatilla, Ranunculus, and Trautvetteria)=- 45_"5`4'<64<'- <<<< <5`<T _`*C<Y5_6`"@Y 4<'_ 5`_"<@5+`+P!<- !['_- C 4`44+" Y 5`+" @Y 5_`" @ <<'C:- 5_5P"''Q4"'- 55@"[#<P[#Y`` 'Y' 5&55 Ranunculus or Trautvetteria as '<<O5P- =<<Hepatica<C* O6P P[<" 56'< Hepatica and Anemone spp. with [@®¤Y66C!6**`P& - x`<- <'FST'- < Clematis and Pulsatilla < '' C655*`"<P"'Anemone !!&45C@6**`P << < STAPFIA: reports 21 © Biologiezentrum Linz/Austria; download unter www.biologiezentrum.