Leader in Biomolecular Solutions for Life Science PDCD6 Rabbit pAb Catalog No.: A6685 1 Publications Basic Information Background Catalog No. This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein A6685 family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in Observed MW T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice 16kDa deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants Calculated MW encoding multiple isoforms have been observed for this gene, and a pseudogene of this 7kDa/21kDa gene is also located on the short arm of chromosome 5. Category Primary antibody Applications WB,IHC,IF,IP Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 10016 O75340 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1). IP 1:50 - 1:200 Synonyms PDCD6;ALG-2;ALG2;PEF1B Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PDCD6 antibody (A6685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PDCD6 antibody (A6685). Western blot was performed from the immunoprecipitate using PDCD6 antibody (A6685) at a dilition of 1:1000. Immunohistochemistry of paraffin- Immunofluorescence analysis of HeLa cells embedded rat kidney using PDCD6 using PDCD6 antibody (A6685). Blue: DAPI antibody (A6685) at dilution of 1:200 (40x for nuclear staining. lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..