TECHNICAL 14 DATA C SHEET Caution: For Laboratory Use. A product for research purposes only. URIDINE DIPHOSPHATE GLUCURONIC ACID, [GLUCURONYL- 14C(U)]- Product Number: NEC414 O LOT SPECIFIC INFORMATION HO O HN 2126226 Lot Number: _____________________ O O N 249 OH Specific Activity: _____________ mCi/mmol OH OH 9213 HO O O O _____________ MBq/mmol OH P P O OO Production Date: _____________16 -Feb -16 OH OH M.W. 580.3 C15 H22 N2O18 P2 PACKAGING : 0.02 mCi/ml (740 KBq/ml) in ethanol : water solutio n (7:3), shipped in dry ice. STABILITY AND STORAGE RECOMMENDATIONS : When uridine diphosphate glucuronic acid, [glucuronyl- 14 C(U)]- is stored at -20 oC in its original solvent and at its original concentration, the rate of decomposition is initially 1% for 12 months from date of purification. Stability is nonlinear and not correlated to isotope half-life. Lot to lot variation may occur. SPECIFIC ACTIVITY RANGE: > 180 mCi/mmol (> 6660 MBq/mmol) RADIOCHEMICAL PURITY: This product was initially found to be greater than 97% when determined by one of the following methods. The rate of decomposition can accelerate. It is advisable to check purity prior to use: High pressure liquid chromatography on a Zorbax SAX column using the following mobile phase: 0.2M ammonium phosphate, pH 3.5 Paper chromatography on Whatman No. 1 using the following solvent systems: a. ethanol : ammonium acetate (1.0M), pH 3.8, (5:2). b. ethanol : ammonium acetate (1.0M), pH 5.0, (7:3). Levels of radiochemical impurities found co-chromatographing with authentic standards were as follows: Less than 0.5% UDP-glucose CHEMICAL PURITY: Determined by ultraviolet spectrophotometry at pH 7.0. Values observed fall into the published range for absorbency ratios for uridine-5'-diphosphate. (1) QUALITY CONTROL: The radiochemical purity of uridine diphosphate glucuronic acid, [glucuronyl-14 C(U)]- is checked at appropriate intervals using the first listed chromatography method. SPECIAL INFORMATION: An aliquot of the entire sample can be taken to dryness by directing a gentle stream of inert gas (nitrogen) over the surface of the solution. The temperature of the solution should not be allowed to exceed 20 oC during the drying process, and the compound should not be permitted to remain in the solid state any longer than necessary. For additional information regarding stability and storage, see the discussion above. REFERENCES: 1. Specifications and Criteria for Biochemical Compounds, Third Edition, (ISBN-0-309-01917-6), National Academy of Sciences, National Research Council, Washington, D.C., 1972. HAZARD INFORMATION: WARNING: This product contains a chemical known to the state of California to cause cancer PerkinElmer, Inc. 549 Albany Street Boston, MA 02118 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com/nenradiochemicals For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. .