AgroLife Scientific Journal - Volume 9, Number 2, 2020 CONCLUSIONS (2016b). Effects of salt stress on three ecologically ISSN 2285-5718; ISSN CD-ROM 2285-5726; ISSN ONLINE 2286-0126; ISSN-L 2285-5718 distinct Plantago species. PLoS ONE, 11(8), e0160236. doi:10.1371/journal.pone.0160236. Halophytic species present in littoral salt Apel, K., Hirt, H. (2004). Reactive oxygen species: DEVELOPMENT OF IN VITRO MULTIPLICATION METHOD marshes of the Mediterranean Spanish coast are metabolism, oxidative stress, and signal transduction. FOR Bidens pilosa L. well adapted to the harsh conditions of their Annual Review of Plant Biology, 55, 373-399. natural habitat and the extreme seasonal Boscaiu, M., Naranjo, M., Vicente, O. (2018). Strategies Lydie VUGUZIGA1, Oana LIVADARIU1, Narcisa BĂBEANU1, Paola ANGELINI2, oscillations in soil salinity and humidity. This to increase crop yields in a climate change scenario. Florentina MATEI1 Scientific Bulletin. Series F. Biotechnologies, 22, 15- adaptation is achieved mainly through the 20. 1 control of ion transport and maintenance of Buchanan, B.B., Gruissem, W., Jones, R.L. (2000). University of Agronomic Sciences and Veterinary Medicine of Bucharest, Faculty of osmotic balance by the accumulation of organic Biochemistry and Molecular Biology of Plants. Biotechnology, 59 Marasti Blvd., District 1, Bucharest, Romania and inorganic osmolytes. Besides, these species American Society of Plant Physiologists, Rockville, 2 University of Perugia, Department of Chemistry, Biology and Biotechnology, appear to possess built-in mechanisms that will MD., USA. Via del Giochetto, 06123, Perugia, Italy Fita, A., Rodríguez-Burruezo, A., Boscaiu, M., Prohens, allow them surviving the potential exacerbation J., Vicente, O. (2015). Breeding and domesticating Corresponding author email: [email protected] of environmental stress factors as a crops adapted to drought and salinity: a new consequence of climate change. These paradigm for increasing food production. Frontiers in mechanisms, observed under artificial Plant Science, 6, 978. doi: 10.3389/fpls.2015.00978. Abstract conditions in the greenhouse, include, for Flowers, T.J., Colmer, T.D. (2008). Salinity tolerance in Bidens pilosa L. it is a medicinal plant which is often used traditionally in the treatment of several diseases. Many example, triggering the synthesis of additional halophytes. New Phytologist, 179, 945-963. pharmaceutical products from this plant have been investigated and these are produced from a very large quantity of + Gil, R., Bautista, I., Boscaiu, M., Lidón, A., Wankhade, plant parts. However, the high production of biomass for this plant is requested. In vitro micropropagation may be a organic osmolytes or K transport to the leaves S., Sánchez, H., Llinares, J., Vicente, O. (2014). valuable solution to obtain multiple uniform plants in a short period of time. This study has been conducted to develop under very high salinities. Responses of five Mediterranean halophytes to such specific in vitro multiplication protocol for Bidens pilosa L. The impacts of basal medium combined with different seasonal changes in environmental conditions. AoB plants growth hormone at various dose have been examined for thedevelopment of three explants (shoot with one nod, ACKNOWLEDGEMENTS Plants, 6, plu049. doi:10.1093/aobpla/plu049 leaves and cotyledons). The cotyledons and leaves transplanted in all tested culture medium didn’t show any Gil, R., Lull, C., Boscaiu, M., Bautista, I., Lidón, A., development during this experiment. The utmost shoot elongation and the many number of roots from shoot tip explants Vicente, O. (2011). Soluble carbohydrates as were achieved on the culture media containing IAA and NAA. IAA at 1 mg/l increased the shoots length at 11.1 cmwith The initial fieldwork was funded by a grant to osmolytes in several halophytes from a 17 rootsfollowed by IAA at 0.5 mg/l (8.3 cm with 12 roots) and NAA at 0.5 mg/l (7.1 cm with 10 roots). The top number O. Vicente from the Spanish Ministry of Mediterranean salt marsh. Notulae Botanicae Horti of the leaves was obtained with the association of BAP and IAA (23 leaves), BAP and NAA (20 leaves) at 0.5 mg/l Science and Innovation (Project CGL2008- Agrobotanici Cluj-Napoca, 39, 9-17. concentrationand the shoots had 7 roots. BAP applied alone at 0.5 mg/l showed a meaningfully role in leaves 00438/BOS), with contribution from the Giorno, F., Wolters-Arts, M., Mariani, C., Rieu, I. multiplication (shoots have 17 leaves) but less effective in lengthening the shoots and roots initiation (shoots have 4 cm (2013). Ensuring reproduction at high temperatures: with 3 roots). The BAP used alone and in combination at 1.0 mg/l inhibit the plant growth. Healthy B. pilosa plants European Regional Development Fund. More The heat stress response during anther and pollen with roots were obtained after 23 days with the help of in-vitro culture and the plants sent in the greenhouse have been recent work was partly supported by Project development. Plants, 2, 489-506. doi: 10.3390/ successfully adapted to the field conditions. AICO/2017/039 from Generalitat Valenciana, plants2030489. granted to M. Boscaiu, and by internal funds of González-Orenga, S., Llinares, J.V., Al Hassan, M., Fita, Key words: Bidens pilosa L., in vitro propagation, medicinal plants, plant growth hormones. Universitat Politècnica de València. A., Collado, F., Lisón, P., Vicente, O., Boscaiu, M. (2020). Physiological and morphological INTRODUCTION (Sundararajan et al., 2006), antimalarial characterization of Limonium species in their natural (Tobinaga et al., 2009), antibacterial REFERENCES habitats: Insights into their abiotic stress responses. Plant and Soil, 449, 267-284. Bidens pilosa Linné (Asteraceae) is an annual (Shandukani et al. 2018), antihypertensive, Al Hassan, M., Estrelles, E., Soriano, P., López-Gresa, Slama, I., Abdelly, C., Bouchereau, A., Flowers, T., herb native to South America thatis spread vasodilatory (Nguelefack et al., 2005; Dimo et M. P., Bellés, J. M., Boscaiu, M., Vicente, O. (2017). Savoure, A. (2015). Diversity, distribution and roles worldwide, especially in tropical and al., 2001) antioxidant and antifungal (Deba et Unraveling salt tolerance mechanisms in halophytes: of osmoprotective compounds accumulated in subtropical regions (Cortés-Rojas et al., 2013). al. 2008), immunomodulatory (Chiang et al., A comparative study on four Mediterranean halophytes under abiotic stress. Annals of Botany, B. pilosa L. isused globally in phototherapy 2007), anti-inflammatory (Horiuchi et al., Limonium species with different geographic 115, 433-447. and each plant part or the whole plant has been 2008), and antiulcerative (Tan et al., 2000). distribution patterns. Frontiers in Plant Science, 8, Vicente, O., Boscaiu, M., Naranjo, M.A., Estrelles, E., 1438. doi: 10.3389/fpls.2017.01438. Bellés, J.M. and Soriano, P. (2004). Responses to salt declared to have an effect in the treatment of Some classes of compounds namely flavonoids, Al Hassan, M., López-Gresa, M.P., Boscaiu, M., stress in the halophyte Plantago crassifolia many illnesses like malaria, flu, cancers, aliphatics, terpenoids, phenylpropanoids, Vicente, O. (2016a). Stress tolerance mechanisms in (Plantaginaceae). Journal of Arid Environments, 58, headache, inflammation, wounds, angina, aromatics, porphyrins were isolated from Juncus: responses to salinity and drought in three 463-481. metabolic syndrome, immunological disorders, Bidens pilosa and are associated in the Juncus species adapted to different natural ***IPCC Intergovernmental panel on climate change. In digestive and infectious diseases etc. (Tan et pharmacological activities of this plant (Wang environments. Functional Plant Biology, 43, 949- Proceedings of the 5th Assessment Report, WGII, al., 2000; Dimo et al., 2001; Young et al., et al., 2010; Silva et al., 2011; Wu et al., 2013; 960. doi: 10.1071/FP16007. Climate Change 2014: Impacts, Adaptation, and Al Hassan, M., Pacurar, A., López-Gresa, M.P., Donat- Vulnerability. Retrieved January, 15, 2020 from 2010). Cortés-Rojas et al., 2013). Torres, M.P., Llinares, J.V., Boscaiu, M., Vicente, O. http://www.ipcc.ch/report/ar5/wg2/. Nowadays, there is an increasing international These pharmaceutical compounds are produced attraction in the usage of B. pilosa and from a very large quantity of plant parts or scientific investigations have revealed that whole plant; moreover, the industrial B.pilosa extracts have many activities such as manufacturers of medicinal substances require antiviral (Chiang et al., 2003), antidiabetic and massive quantities of biomass, which is a antihyperglycemic (Hsu et al., 2009), antitumor 377 difficulty in countries where certain species are Naphthaleneacetic acid) alone and in longest shoot and the many number of roots concentration (0.5 mg/l). It is also clearly that rare (Ashish & Anchal, 2014). combination at the concentration of 0.5 mg/l were produced in medium supplemented with IAA is more effective in advancing shoot Understandably, the more the demand for and 1.0 mg/l. The 9 variants of hormonal IAA and NAA as it is detailed in the Figure length, root initiation and multiplication than bioactive compounds increases, the more the combinations namely:M2 = MS+BAP(0.5 1and Figure 2. The basal medium NAA and BAP. De Lima et al. (2017), reported exploitation of medicinal plants also increases. mg/l), M3 = MS+BAP(1 mg/l), M4 = MS+3 supplemented with IAA at 1.0 mg/l has the top that increasing the IAA concentration favoured Thus, researches