Piscine orthoreovirus Series of dissertations at the Norwegian University of Life Sciences Thesis number 79 Viruses, not lions, tigers or bears, sit masterfully above us on the food chain of life, occupying a role as alpha predators who prey on everything and are preyed upon by nothing Claus Wilke and Sara Sawyer, 2016 1.1. Background............................................................................................................................................... 1 1.2. Piscine orthoreovirus................................................................................................................................ 2 1.3. Replication of orthoreoviruses................................................................................................................ 10 1.4. Orthoreoviruses and effects on host cells ............................................................................................... 18 1.5. PRV distribution and disease associations ............................................................................................. 24 1.6. Vaccine against HSMI ............................................................................................................................ 29 4.1. The non ......................................................37 4.2. PRV causes an acute infection in blood cells ..........................................................................................40 4.3. DNA vaccine expressing the PRV non-structural proteins......................................................................45 5.1. Plasmid construction...............................................................................................................................53 5.2. Transfection experiments and epitope tagging........................................................................................54 5.3. Production of polyclonal antibodies........................................................................................................55 5.4. Immunoprecipitation ...............................................................................................................................56 5.5. Protein detection .....................................................................................................................................57 5.6. RT-qPCR .................................................................................................................................................60 5.7. Experimental challenges .........................................................................................................................61 Piscine orthoreovirus Authors: Haatveit HM, Nyman IB, Markussen T, Wessel Ø, Dahle MK, Rimstad E. Published: Veterinary Research 2016, 47:5 Piscine orthoreovirus Authors: Haatveit HM, Wessel Ø, Markussen T, Lund M, Thiede B, Nyman IB, Braaen S, Dahle MK, Rimstad E. Published: Viruses 2017, 9, 49 Piscine orthoreovirus Salmo salar Authors: Haatveit HM, Hodneland K, Braaen S, Hansen EF, Frost P, Rimstad E. Manuscript Piscine orthoreovirus Salmo salar Reoviridae Mammalian orthoreovirus Piscine orthoreovirus Reoviridae, Mammalian orthoreovirus Background Salmo salar Piscine orthoreovirus Piscine orthoreovirus Reoviridae, Orthoreovirus orthoreovirus Reoviridae Reoviridae Reoviridae Spinareovirinae Sedoreovirinae Orthoreovirus Spinareovirinae Mammalian orthoreovirus Avian orthoreovirus Baboon orthoreovirus Nelson Bay orthoreovirus Reptilian orthoreovirus Piscine orthoreovirus Broome orthoreovirus Piscine orthoreovirus.