The following supplement accompanies the article Linking habitat and life history for conservation of the rare saltmarsh topminnow Fundulus jenkinsi: morphometrics, reproduction, and trophic ecology John D. Lopez1, 3, Mark S. Peterson1,*, Erik T. Lang1, Adriana M. Charbonnet2 1Department of Coastal Sciences, The University of Southern Mississippi, Ocean Springs, Mississippi 39564, USA 2Ocean Springs High School, Ocean Springs, Mississippi 39564, USA 3Present address: Texas Parks and Wildlife, Coastal Fisheries Division, Brownsville Field Office, Brownsville, Texas 78520, USA *Corresponding author. Email: [email protected] Endangered Species Research 12: 141–155 (2010) Supplement 1. Summary of the data used in the multi-dimensional scaling (MDS) analysis Table S1. Spring mean volume (µl) of prey items by size class (15, 20, … 40 mm) and replicate number (01 or 02) used for dietary analysis. Data are the mean value of prey volumes of all fish in each size class and replicate. P: Phylum, SP: sub-phylum, C: class, SC: sub-class, O: order, SO: sub-order, F: family, G: genus, Unid: unidentified, —: not in diet Prey size (mm) and replicate number Taxon 15–01 15–02 20–01 20–02 25–01 25–02 30–01 30–02 35–01 35–02 40–01 40–02 P. Acanthocephala –––––––––––– P. Nematoda –––––––––––– P. Mollusca C. Gastropoda – – 0.063 ––––––––– C. Polychaeta –––––––––––– C. Oligochaeta –––––––––––0.250 P. Arthropoda C. Arachnida ––––––0.140 – – 0.002 SO. Hydracarina – 0.031 –––––––––– SP. Crustacea –––––––––––– C. Maxillopoda SC. Copepoda 0.200 0.200 – 0.154 –––––––– C. Ostracoda – – 0.125 – – – 0.001 ––––– C. Malacostraca O. Amphipoda 0.200 0.200 – 0.074 0.240 0.087 0.356 0.012 0.179 – 0.098 – F. Melitadae ––––––––0.029 – – – F. Corophiidae –––––––––––– G. Apocorophium 0.200 –––––––0.025 – – – O. Isopoda –––––––––––– O. Mysidacea –––––––––––– O. Decapoda F. Penaeidae –––––––––––– Crab zoea –––––––––––– F. Portunidae G. Callinectes –––––––––––– F. Palaemonidae –––––––––––– Unid decapod –––––––––0.200 – 0.243 C. Insecta SC. Apterygota O. Collembola – 0.369 – 0.155 –––––––– SC. Pterygota O. Odonata – 0.109 ––––––––0.237 – O. Orthoptera –––––––0.119 – 0.083 – – O. Hemiptera – – 0.125 0.103 0.187 0.309 0.003 0.336 – 0.401 – 0.079 O. Megaloptera –––––––––0.002 – – O. Lepidoptera –––––––––––– O. Coleoptera –––––––––––0.257 O. Diptera – 0.091 0.187 0.093 0.036 0.213 0.152 0.212 0.011 0.018 0.040 0.102 F. Chironomidae –––––––––––– O. Hymenoptera 0.200 ––––––––0.076 – – 2 Table S1(continued) Prey size and replicate number Taxon 15–01 15–02 20–01 20–02 25–01 25–02 30–01 30–02 35–01 35–02 40–01 40–02 O. Thysanoptera –––––––––––– Unid arthropod ––––0.329 – – 0.006 – 0.019 – – Arthropod parts – – 0.250 0.301 – 0.386 0.084 0.315 0.003 0.200 – 0.067 Plant detritus ––––––0.214 ––––– Detritus –––––––––––– Amorphous – – – 0.117 0.171 0.005 0.189 – 0.612 – 0.624 – Eggs 0.200 – 0.250 ––––––––– Crab zoea/eggs –––––––––––– Table S2. As in Table S1, but for summer Prey size and replicate number Taxon 15–01 15–02 20–01 20–02 25–01 25–02 30–01 30–02 35–01 35–02 40–01 40–02 P. Acanthocephala –––––––––0.014 – – P. Nematoda –––––––––0.001 – – P. Mollusca C. Gastropoda ––––––––––0.077 0.037 C. Polychaeta ––––––––––0.004 0.002 C. Oligochaeta – – 0.013 – – 0.071 –––––– P. Arthropoda C. Arachnida 0.043 0.007 0.014 0.107 0.079 0.014 0.009 0.038 0.011 0.042 – 0.115 SO. Hydracarina –––––0.005 0.002 ––––– SP. Crustacea ––––––––0.027 – – – SC. Copepoda – 0.167 – 0.146 –––––––– C. Ostracoda 0.002 – 0.003 ––––––0.001 – – C. Malacostracea O. Amphipoda – 0.052 – 0.010 0.128 0.031 – – 0.037 0.016 0.077 0.052 F. Melitadae –––––––––––– F. Corophiidae ––––0.002 ––––––– G. Apocorophium – – – 0.042 –––––––– O. Isopoda – – – <0.001 0.034 – – – 0.008 – – – O. Mysidacea 0.091 ––––––––––– O. Decapoda F. Penaeidae –––––––––0.071 – – Crab zoea 0.182 0.187 0.039 0.225 0.310 0.323 0.162 0.161 0.077 0.009 – 0.071 F. Portunidae G. Callinectes – 0.018 –––––––––– F. Palaemonidae ––––––––––0.074 – Unid decapod – 0.038 – 0.046 – – 0.246 ––––– C. Insecta SC. Apterygpta O. Collembola – – 0.005 0.083 – 0.039 – 0.111 – 0.012 0.006 – SC. Pterygota O. Odonata –––––––0.111 – – 0.028 0.020 O. Orthoptera – – 0.071 – 0.071 – – – 0.062 – – – O. Hemiptera 0.317 0.190 0.329 0.073 0.144 0.016 0.018 0.207 0.289 0.156 0.039 0.141 O. Megaloptera –––––––––––– O. Lepidoptera – – 0.057 ––––––––– O. Coleoptera – – – 0.008 <0.001 ––––0.030 – 0.001 O. Diptera 0.080 0.084 0.071 0.006 0.001 0.044 0.160 0.037 0.090 0.087 0.233 0.049 F. Chironomidae 0.006 0.021 0.026 – 0.001 0.037 – 0.002 – 0.068 – – O. Hymenoptera – 0.052 0.006 – – 0.162 – 0.007 0.085 0.055 0.062 0.026 O. Thysanoptera – – 0.012 ––––––––– Unid arthropod – 0.021 – 0.025 – – 0.125 ––––0.088 Arthropod parts 0.280 0.125 0.223 0.042 0.155 0.171 0.153 0.325 0.237 0.211 0.262 0.355 Plant detritus ––––––––––0.073 – Detritus –––––0.071 – – – 0.112 – – Amorphous – 0.027 0.130 0.058 0.075 0.016 0.125 – 0.077 0.117 0.064 0.042 Eggs – 0.011 –––––––––– Crab zoea/eggs – – – 0.130 –––––––– 3 Table S3. As in Table S1, but for winter Prey size and replicate number Taxon 15–01 15–02 20–01 20–02 25–01 25–02 30–01 30–02 35–01 35–02 40–01 40–02 P. Acanthocephala –––––––––––– P. Nematoda –––––––––––– P. Mollusca C. Gastropoda –––––––––––– C. Polychaeta –––––––––––– C. Oligochaeta –––––––––––0.047 P. Arthropoda C. Arachnida ––––––0.056 ––––– SO. Hydracarina – 0.026 – – – 0.001 –––––– SP. Crustacea –––––––––––– C. Maxillopoda SC. Copepoda –––––––––––– C. Ostracoda –––––––––––– C. Malacostracea O. Amphipoda 0.267 – 0.462 0.153 0.920 0.325 0.795 0.394 0.166 0.200 0.110 0.091 F. Melitadae –––––––––––– F. Corophiidae –––––––––––– G. Apocorophium –––––––––––– O. Isopoda –––––––––––– O. Mysidacea –––––––––––– O. Decapoda F. Penaeidae –––––––––––– Crab zoea –––––––––––– F. Portunidae G. Callinectes –––––––––––– F. Palaemonidae –––––––––––– Unid. decapod ––––––––––0.140 – C. Insecta SC. Apterygota O. Collembola – 0.574 0.044 – 0.002 – 0.003 – 0.109 – – – SC. Pterygota O. Odonata –––––––––––– O. Orthoptera –––––––––––– O. Hemiptera 0.500 – – 0.097 – 0.150 0.010 0.200 – 0.351 0.048 0.169 O. Megaloptera – – – – – ––––––– O. Lepidoptera – – – – – ––––––– O. Coleoptera –––––––0.046 –––– O. Diptera – – 0.163 0.244 0.078 0.258 0.137 0.241 0.475 0.326 0.452 0.424 F. Chironomidae –––––––––––0.010 O. Hymenoptera – – – 0.006 – ––––––0.009 O. Thysanoptera –––––––––––– Unid arthropod – – 0.024 – – ––––––– Arthropod parts 0.233 – 0.307 0.442 – 0.266 – 0.119 – – 0.250 0.250 Plant detritus –––––––––––– Detritus –––––––––0.022 – – Amorphous – 0.400 – – – – – – 0.250 0.101 – – Eggs – – – 0.058 – ––––––– Crab zoea/eggs ––––––––––––.