International Journal of Pharmacy and Biological Sciences-IJPBSTM (2019) 9 (3):220-224 Online ISSN: 2230-7605, Print ISSN: 2321-3272 Research Article | Biological Sciences | Open Access | MCI Approved UGC Approved Journal Analysis of Proximate Composition of Tenualosa Ilisha (Clupeiformes: Clupeidae) in Narmada River, Gujarat Kangkan Jyoti Sarma and Pradeep C. Mankodi* Division of Freshwater and Marine Biology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara-390002, Gujarat. Received: 10 Mar 2019 / Accepted: 9 Apr 2019 / Published online: 1 Jul 2019 Corresponding Author Email: [email protected] Abstract In the present study, the proximate biochemical compositions of Tenualosha ilisha (Hilsa, Shad) have been analysed. Hilsa, a species related to Clupeidae family is an anadromous fish migrating from the Persian Gulf along the Indian Subcontinent up to Bangladesh have been found majorly inhabiting the waters of Narmada River of Gujarat normally inhabiting the lower region of the estuarine and foreshore areas during the monsoon for breeding. The analysis of protein, ash, fat and moisture content were done in relation to different body weight groups of Hilsa. Samples were collected from major landing stations of Narmada River, viz., Jageshwar, Ambetha, Vegni, Bhadbhut and Hansot villages of Bharuch district. The protein content in females in pre- monsoon was found to be higher than the males. The samples processed for protein and fat in post monsoon period were found to be vice versa for the fact that the females had relatively utilised it during the spawning and breeding period. Similarly, the ash and moisture content have also showed profound changes in tissue samples processed during pre- and post-monsoon period for both males and females. Keywords Tenualosha ilisha, proximate analysis, Narmada River, Gujarat. ***** INTRODUCTION potamodromous type and a marine kind have been Hilsa (Tenualosha ilisha), a species related to perceived [1][2][3][4][5]. The potamodromous Clupeidae family is an anadromous fish migrating stocks seem to stay in the centre scopes of the from the Persian Gulf along the Indian Subcontinent streams consistently and breed in that. The up to Bangladesh have been found majorly inhabiting anadromous stocks, whose typical living space is the the waters of Narmada River of Gujarat normally lower locale of the estuaries and the foreshore inhabiting the lower region of the estuarine and zones, climb the waterways amid the reproducing foreshore areas during the monsoon for breeding. season and come back to the first natural The Hilsa shad is to a great extent an anadromous surroundings subsequent to bringing forth [6]. The species, however two different ecotypes, a fluvial upstream movement amid the principle rearing DOI: https://doi.org/10.21276/ijpbs.2019.9.3.31 Kangkan Jyoti Sarma and Pradeep C. Mankodi* 220 www.ijpbs.com or www.ijpbsonline.com ISSN: 2230-7605 (Online); ISSN: 2321-3272 (Print) Int J Pharm Biol Sci. season depends to a great extent on the beginning of The fresh fishes were collected from major landing the south‐west rainstorm and subsequent flooding of stations of Narmada River, viz., Jageshwar, Ambetha, the significant waterways of India, Bangladesh and Vegni and Hansot (21°34’54.50” N and Burma [3].It has been set up that the data on the 72°48’24.80”E) Bhadbhut (21°40’54.29”N and substance synthesis of fish in regard to the nutritive 72°50’42.28”E) (Fig. 1) as the primary landing esteem is essential to contrast and other wellspring centres/villages of Bharuch district in the monsoon of creature protein, meat and poultry items [7]. concerning on the main focus on seasonal variation There are a couple of reports on the nutritive for its anadromous behaviour. Fishes were collected estimations of Hilsa. Hilsa is a transitory fish and it in early evening as soon as they arrive from fishing voyages a large number of kilometers from upstream and after collection fish samples were taken in ice of stream to mid sea. Various locales of the Narmada box and were transferred to laboratory of River and the mouth of the River contains various Department of Science, Faculty of Science, The kinds of nourishment (phyto‐ and Maharaja Sayajirao University of Baroda, Vadodara zooplankton)[8].Different types of food make for analysis. After washing with tap water and taking proximate composition a bit different and also length and weight of the fishes, the sample fish were different part of the body makes the composition frozen at -20ºC until laboratory process. The samples different as well.Biochemical composition of fish were dissected out and the muscle tissues for the flesh may vary within the same species of fish study were taken from the caudal region. The study depending upon the fishing season, age, sex and is currently going on for the other regions as well so habitat [9][10][11]. The variation is also found within that the proximate content as per body difference the different region of the body. In fishes, proximate can be ascertained. The proximate composition of composition means the composition of the fish flesh. fish was determined by conventional methods of Fish flesh contains four basic ingredients in varying Association of Official Analytical Chemicals [12] on proportions major nutrients such as water (70 ‐ 80%), weight basis. protein (18 ‐ 20%), fat (5%) and minerals (5%) and At the beginning, initial weight of the sample (W1) minor nutrients such as vitamin, carbohydrate. It has was taken. The collected samples were dried in an high nutritional value in terms of fats and proteins oven at about 105°C for about 3 hours and were that are not commonly available in other foods. The transferred to desiccator to cool down. Thereafter present study details with the proximate analysis of the weight of the sample (W2) was taken again. The protein, fat, moisture and ash content in Hilsa. moisture content was calculated using the following equation: Moisture (%) = (W1 – W2) x 100/W1 [12]. MATERIALS AND METHODS Fig 1. Moisture content (%) variation for male and female Hilsa collected from different places. The range of moisture content (%) for male specimen ranged from 37.2 – 64.1% while for the female range starts from 49.4 – 72.5%. MOISTURE CONTENT 80 70 60 50 40 Male 30 Percentage (%) Percentage Female 20 10 0 Jageshwar Ambetha Vegni Hansot Bhadbhut Area of catch International Journal of Pharmacy and Biological Sciences Kangkan Jyoti Sarma and Pradeep C. Mankodi* 221 www.ijpbs.com or www.ijpbsonline.com ISSN: 2230-7605 (Online); ISSN: 2321-3272 (Print) Int J Pharm Biol Sci. Fig 2. Ash content (%) variation for male and female Hilsa collected from different places. The range of ash content (%) for male specimen ranged from 0.8 – 1.33% while for the female range starts from 0.9 – 1.16%. ASH CONTENT Male 1.4 Female 1.2 1 0.8 0.6 0.4 Percentage (%) Percentage 0.2 0 Jageshwar Ambetha Vegni Hansot Bhadbhut Area of catch Fig 3. Protein content (%) variation for male and female Hilsa collected from different places. The range of protein content (%) for male specimen ranged from 29.5 – 41% while for the female range starts from 33.2 – 49.5%. PROTEIN CONTENT Male Female 60 50 40 30 20 Percentage (%) Percentage 10 0 Jageshwar Ambetha Vegni Hansot Bhadbhut Area of catch Table 4. Fat content (%) variation for male and female Hilsa collected from different places. The range of fat content (%) for male specimen ranged from 16 – 29.5% while for the female range starts from 21. – 41.2%. FAT CONTENT Male 50 Female 40 30 20 Percentage (%) Percentage 10 0 Jageshwar Ambetha Vegni Hansot Bhadbhut Area of catch International Journal of Pharmacy and Biological Sciences Kangkan Jyoti Sarma and Pradeep C. Mankodi* 222 www.ijpbs.com or www.ijpbsonline.com ISSN: 2230-7605 (Online); ISSN: 2321-3272 (Print) Int J Pharm Biol Sci. The protein content of the fish was determined by CONCLUSION micro kjeldahl method. It involves conversion of There were numerous conceivable factors, for organic nitrogen to ammonium sulphate by digestion example, measure, sex, development of tests that with concentrated sulphuric acid in a microkjeldahl can influence the contrasts in proximate analysis of flask [12]. fishes. Examining strategies additionally assumed For the estimation of fat content, the dried samples critical job in the distinctions of the discoveries. One left after moisture determination were finely grinded of conceivable components was the and the fat was extracted with a nonpolar solvent, representativeness of the examples. Unique ethyl ether. After extraction, the solvent was approach of inspecting methods, which included the evaporated, and the extracted materials were technique for sample gathering, distinctive example weighed [12]. accumulation locales, and distinction in the The ash content of a sample is the residue left after incorporation criteria of samples with different past ashing in a furnace at about 550 ‐ 600°C till the examinations, clarified the slight contrast in the residue became white [12]. proximate analysis. There was likewise sure data in past examinations that was lacking to be utilized for RESULTS AND DISCUSSIONS examination with the present investigation. Thus, it The analysis of the samples for moisture, ash, protein is hopeful that details on the sampling procedures and fat for the muscle tissues were carried out and and methods of analysis used in this study will be the results obtained have been represented able to provide sufficient information for any graphically with reference to male and female hilsa comparative purposes in the future. caught during the monsoon season while travelling into the freshwaters of Narmada River for spawning ACKNOWLEDGEMENTS activity. The authors are thankful to the Head, Department of The results obtained from the studies showed the Zoology for giving us the necessary field permissions highest percentage (%) of moisture from the muscles and allowing us to use the laboratory facilities during of the samples.