UK Standards for Microbiology Investigations 2018 Potassium hydroxide test DECEMBER 7 TO NOVEMBER 23 BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Issued by the Standards Unit, National Infection Service, PHE Bacteriology – Test Procedures | TP 30 | Issue no: df+ | Issue date: xx | Page: 1 of 13 © Crown copyright 2018 Potassium hydroxide test Acknowledgments UK Standards for Microbiology Investigations (UK SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. UK SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). 2018 The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the medical editors for editing the DECEMBER medical content. 7 For further information please contact us at: TO Standards Unit National Infection Service Public Health England 61 Colindale Avenue NOVEMBER London NW9 5EQ 23 E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratories BETWEEN PHE publications gateway number: 2018381ON UK Standards for Microbiology Investigations are produced in association with: CONSULTED WAS DOCUMENT THIS - DRAFT Logos correct at time of publishing. Bacteriology – Test Procedures | TP 30 | Issue no: df+ | Issue date: xx | Page: 2 of 13 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Potassium hydroxide test Contents Acknowledgments ................................................................................................................. 2 Contents ................................................................................................................................. 3 Amendment table ................................................................................................................... 4 UK SMI: scope and purpose .................................................................................................. 5 Scope of document ................................................................................................................2018 7 Introduction ............................................................................................................................ 7 Technical information/limitations ......................................................................................... 7 DECEMBER 7 1 Safety considerations ................................................................................................ 8 TO 2 Reagents and equipment ........................................................................................... 8 3 Quality control organisms ......................................................................................... 8 4 Procedure and results ................................................................................................NOVEMBER 8 23 Appendix: Potassium hydroxide test ................................................................................. 10 References ........................................................................................................................... 11 BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Bacteriology – Test Procedures | TP 30 | Issue no: df+ | Issue date: xx | Page: 3 of 13 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Potassium hydroxide test Amendment table Each UK SMI method has an individual record of amendments. The current amendments are listed on this page. The amendment history is available from [email protected]. New or revised documents should be controlled within the laboratory in accordance with the local quality management system. Amendment number/date 2018 Issue number discarded Insert issue number Anticipated next review date* DECEMBER 7 Section(s) involved Amendment TO Document updated. Technical limitations updated with subheadings. Whole document. References updated withNOVEMBER grades. 23 Modified GRADE table added in the references section to explain the grades given to references. Alternative NCTC control strains that could be BETWEEN used have been added and updated in the Quality control organisms. document.ON These have also been validated by NCTC. Reviews can be extended up to fiveCONSULTED years subject to resources available. WAS DOCUMENT THIS - DRAFT Bacteriology – Test Procedures | TP 30 | Issue no: df+ | Issue date: xx | Page: 4 of 13 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Potassium hydroxide test UK SMI: scope and purpose Users of UK SMIs Primarily, UK SMIs are intended as a general resource for practising professionals operating in the field of laboratory medicine and infection specialties in the UK. UK SMIs also provide clinicians with information about the available test repertoire and the standard of laboratory services they should expect for the investigation of infection in their patients, as well as providing information that aids the electronic ordering of appropriate tests. The documents also provide commissioners of healthcare services with the appropriateness and standard of microbiology investigations they should be2018 seeking as part of the clinical and public health care package for their population. Background to UK SMIs UK SMIs comprise a collection of recommended algorithms and proceduresDECEMBER covering all stages of the investigative process in microbiology from the pre-analytical7 (clinical syndrome) stage to the analytical (laboratory testing) and post analyticalTO (result interpretation and reporting) stages. Syndromic algorithms are supported by more detailed documents containing advice on the investigation of specific diseases and infections. Quality guidance notes describe laboratory processes which underpin quality, for example assay validation. NOVEMBER Standardisation of the diagnostic process through the23 application of UK SMIs helps to assure the equivalence of investigation strategies in different laboratories across the UK and is essential for public health surveillance, research and development activities. Equal partnership working BETWEEN UK SMIs are developed in equal partnershipON with PHE, NHS, Royal College of Pathologists and professional societies. The list of participating societies may be found at https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratorieshttp://www.hpa-standardmethods.org.uk/. Inclusion of a logo in an UK CONSULTEDSMI indicates participation of the society in equal partnership and support for the objectives and process of preparing UK SMIs. Nominees of professionalWAS societies are members of the Steering Committee and working groups which develop UK SMIs. The views of nominees cannot be rigorously representative of the members of their nominating organisations nor the corporate views of their organisations. Nominees act as a conduit for two way reporting and dialogue. Representative views are sought through the consultation process. UK SMIs DOCUMENT are developed, reviewed and updated through a wide consultation process. THIS Quality- assurance NICE has accredited the process used by the UK SMI working groups to produce UK DRAFTSMIs. The accreditation is applicable to all guidance produced since October 2009. The process for the development of UK SMIs is certified to ISO 9001:2008. UK SMIs represent a good standard of practice to which all clinical and public health microbiology laboratories in the UK are expected to work. UK SMIs are NICE accredited and represent neither minimum standards of practice nor the highest level Microbiology is used as a generic term to include the two GMC-recognised specialties of Medical Microbiology (which includes Bacteriology, Mycology and Parasitology) and Medical Virology. Bacteriology – Test Procedures | TP 30 | Issue no: df+ | Issue date: xx | Page: 5 of 13 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Potassium hydroxide test of complex laboratory investigation possible. In using UK SMIs, laboratories should take account of local requirements and undertake additional investigations where appropriate. UK SMIs help laboratories to meet accreditation requirements by promoting high quality practices which are auditable. UK SMIs also provide a reference point for method development. The performance of UK SMIs depends on competent staff and appropriate quality reagents and equipment. Laboratories should ensure that all commercial and in-house tests have been validated and shown to be fit for purpose. Laboratories should participate in external quality assessment schemes and undertake relevant internal quality control procedures. Patient and public involvement 2018 The UK SMI working groups are committed to patient and public involvement in the development of UK SMIs. By involving the public, health professionals, scientists and voluntary organisations the