PLRG1 Antibody Cat. No.: 46-203 PLRG1 Antibody 46-203 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Specifications HOST SPECIES: Goat SPECIES REACTIVITY: Human, Mouse HOMOLOGY: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow IMMUNOGEN: The immunogen for this antibody is: C-PVSWKPEIIKRKRF TESTED APPLICATIONS: ELISA, IHC, WB Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse APPLICATIONS: NP_058064.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml. September 28, 2021 1 https://www.prosci-inc.com/plrg1-antibody-46-203.html This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 SPECIFICITY: (NP_001188493.1). POSITIVE CONTROL: 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: PLRG1 PLRG1, pleiotropic regulator 1 (PRL1 homolog, Arabidopsis), PRL1, MGC110980, ALTERNATE NAMES: pleiotropic regulator 1 (PRL1, Arabidopsis homolog), pleiotropic regulator 1 (PRL1homolog, Arabidopsis), pleiotropic regulator 1 (PRL1, Arabidopsis homolog) ACCESSION NO.: NP_002660.1, NP_001188493.1 PROTEIN GI NO.: 4505895 GENE ID: 5356 Background and References 1) Ajuh P, Lamond AI Identification of peptide inhibitors of pre-mRNA splicing derived REFERENCES: from the essential interaction domains of CDC5L and PLRG1 Nucleic Acids Res. 2003 Nov 1;31(21):6104-16 ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 2 https://www.prosci-inc.com/plrg1-antibody-46-203.html.