3.3 NGC™ Chromatography Systems and ChromLab™ Software Installation Guide Version 3.3 NGC™ Chromatography Systems and ChromLab™ Software Installation Guide Version 3.3 Bio-Rad Technical Support Department The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific time. Go to www.consult.bio-rad.com for worldwide technical support. Phone: 1-800-424-6723, option 2 Web: www.consult.bio-rad.com Email: [email protected] (U.S./Canada only) For technical assistance outside the U.S. and Canada, contact your local technical support office. Notice No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad. Bio-Rad reserves the right to modify its products and services at any time. This instrument guide is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors or omissions, or for any damage resulting from the application or use of this information. Intel Core is a trademark of Intel Corporation PEEK is a trademark of Victrex plc. Microsoft, SQL Server, and Windows are trademarks of Microsoft Corporation. Copyright © 2015 by Bio-Rad Laboratories. All rights reserved. Table of Contents Chapter 1 Introduction. .7 Main Features . 8 Safety Information. 9 Safety Alerts . 9 Safety Precautions . 9 Regulatory Information . 10 Safety Compliance . 10 Electromagnetic Compatibility (EMC) . 10 FCC Warning and Notes . 11 Finding Out More . 12 Chapter 2 Preparing Your Site . .13 Delivery Crate Dimensions . 13 Room Requirements . 15 Bench Space. 15 Environment . 16 Power Requirements. 17 ChromLab System Requirements. 18 Preparing the ChromLab Computer . 19 Chapter 3 Setting Up NGC Chromatography Systems . .21 NGC Systems Accessories Kit . 22 NGC Scout Accessories Kit . 23 NGC Discover Accessories Kit . 23 NGC Discover Pro Accessories Kit. 23 Installation Guide | iii Table of Contents Attaching the Touch Screen . 24 NGC System Location Is on a Lab Bench. 24 NGC System Location Is a Standard Cold Cabinet . 34 Connecting the Drain Tube to the Buffer Tray . 39 Installing the pH Probe in the pH Module . 42 Attaching an Expansion Tier to the NGC Instrument . 44 Inserting Additional Modules. 51 Connecting Peripheral Equipment . 56 Connecting the BioFrac Fraction Collector . 56 Connecting Column Clamps to the NGC Instrument . 59 Connecting Tubing to the Columns. 60 Connecting a Keyboard or Mouse. 61 Connecting the Air Sensors. 62 Connecting External Devices to the NGC Instrument . 66 Connecting the Power and Communication Cables . 76 Connecting the NGC System to the ChromLab Computer. 78 Establishing a Direct Cable Connection . 78 Establishing a Network Connection . 79 Chapter 4 Installing ChromLab Software . 81 Installing ChromLab Software . 82 Establishing the Connection Between ChromLab Software and the NGC Systems . 84 Powering on the NGC Instrument and Peripherals . 84 Setting the System Name . 86 Setting Delay Volume . 92 Setting Up Remote Access Connection . 95 iv | NGC Chromatography Systems and ChromLab Software Table of Contents Chapter 5 Plumbing and Priming the NGC Chromatography Systems . 97 Preparing the Buffer and Waste Bottles . 98 Buffer Bottles. 98 Waste Bottles . 98 Plumbing NGC Systems . 99 General Guidelines for Tubing Sizes . 99 Plumbing All NGC Systems. 100 Plumbing the NGC Quest System. 106 Plumbing the NGC Scout System . 109 Plumbing the NGC Discover System . 113 Plumbing the NGC Discover Pro System . 118 Priming and Purging the System. 123 Priming and Purging the NGC Quest System . 124 Priming and Purging the NGC Scout System . 127 Priming and Purging the NGC Discover System. 131 Calibrating the Instrument . 144 Calibrating pH . 144 Calibrating the Pump Flow Rate . 145 Calibrating Pressure Sensor Zero Point . 145 Calibrating the Conductivity Monitor. 146 Appendix A Upgrading ChromLab Software and NGC Systems 147 Upgrading ChromLab Software . 147 Upgrading ChromLab Software to the Current Version . 147 Resetting Customized System Settings . 148 Updating NGC Systems . 148 Installation Guide | v Table of Contents Appendix B Troubleshooting ChromLab Software Installation . 151 Possible Causes for Connection Failures . 151 Configuring the Network Adapter for Direct Connection . 154 Troubleshooting Network Connection Issues . 158 Locating the IP Address of the NGC Instrument. 158 Checking the Network Connection . 160 ChromLab Software Firewall Settings. 162 Firmware Update Failure . 163 Appendix C Connecting the C-96 Autosampler to NGC Systems. 165 Using the C-96 Autosampler with NGC Systems . 165 Connecting the C-96 Autosampler to the NGC Instrument . 165 Plumbing and Priming the C-96 Autosampler. 168 Setting Up the C-96 Autosampler for Use in ChromLab Methods . 172 Appendix D Firewall Configuration . ..