Journal of Helminthology Description of a new species Fuhrmannetta jurubatensis n. sp. (Cestoda, Davaineidae) cambridge.org/jhl from Cerradomys goytaca Tavares, Pessôa & Gonçalves, 2011 (Rodentia, Cricetidae) Research Paper 1 1 2 Cite this article: Oliveira LC, Oliveira FCR, L.C. Oliveira , F.C.R. Oliveira and N.B. Ederli Ederli NB. Description of a new species 1 Fuhrmannetta jurubatensis n. sp. (Cestoda, Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Davaineidae) from Cerradomys goytaca Av. Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ, Brazil, 28013-602 and Tavares, Pessôa & Gonçalves, 2011 (Rodentia, 2Instituto do Noroeste Fluminense de Educação Superior (INFES), Universidade Federal Fluminense (UFF), Cricetidae). Journal of Helminthology https:// Av. João Jasbick, s/n, Aeroporto, Santo Antônio de Pádua, RJ, Brazil, 28.470-000 doi.org/10.1017/S0022149X17000773 Received: 5 April 2017 Abstract Accepted: 31 July 2017 A new species of cestode of the genus Fuhrmannetta found in the small intestine of Author for correspondence: Cerradomys goytaca is described herein, named Fuhrmannetta jurubatensis n. sp. Rodents N.B. Ederli, E-mail: [email protected] were collected from the sand-plains areas of the northern coast of the State of Rio de Janeiro, Brazil. Morphological analyses were conducted by light and scanning electron micros- copy. From the morphological analysis and a comparison with the known species of the genus, F. jurubatensis n. sp. can be identified by a combination of morphological and morpho- metrical characteristics, including strobila length, number and length of rostellar hooks, pos- ition of the genital pore and the number of eggs per uterine capsule. Introduction Tavares et al.(2011) described a new species of rodent of the family Cricetidae, Cerradomys goytaca Tavares, Pessôa & Gonçalves, 2011 from south-eastern Brazil. This species lives on sand-plains areas of the northern coast of Rio de Janeiro State and the south of Espírito Santo State. The differentiation of the new species was based on its skull morphology, coat and chromosomal differences (Tavares et al., 2011). According to these authors, this new taxon has a restricted distribution to the sand-plains area, forming an open mosaic on the coast of the states of Rio de Janeiro and Espírito Santo. The cestodes of the genus Raillietina Fuhrmann, 1920 (Cyclophyllidea, Davaineidae) are divided into four subgenera by Fuhrmann (1920), i.e. R. (Raillietina) Fuhrmann, 1920, R. (Fuhrmannetta) Stiles & Orleman, 1926, R. (Skrjabinia) Fuhrmann, 1920 and R. (Paroniella) Fuhrmann, 1920. Movsesyan (1966) elevated these subgenera to the generic rank and this concept has been accepted in the latest taxonomic revision of the family Davaineidae Braun, 1900 (Jones & Bray, 1994). The main diagnostic characteristics of the gen- era Raillietina, Fuhrmannetta, Skrjabinia and Paroniella are the number of eggs per uterine capsule and the position of the genital pore, e.g. unilateral or irregularly alternating. According to Jones & Bray (1994), the genus Fuhrmannetta is characterized by the pres- ence of irregularly alternating genital pores and uterine capsules containing 3–5 eggs. Species of this genus have been reported parasitizing birds and some mammals, with a cosmo- politan distribution (Sawada, 1965; Rolas, 1976; Olsen & Kuntz, 1979; Schmidt, 1986; Movsesyan, 2003a, b). During studies on the helminth parasites of the cricetid rodent C. goytaca from the Jurubatiba Natural Reserve, in the city of Quissamã, State of Rio de Janeiro, Brazil, we found cestode specimens belonging to the family Davaineidae. Our observations revealed that they belong to a new species of the genus Fuhrmannetta. This study aimed to describe this new species from C. goytaca from Brazil. Materials and methods A total of 16 rodents C. goytaca were collected between April and December 2015, in sand- plains areas of Quissamã city, in the northern region of Rio de Janeiro State, using livetraps and lures made of fruits, seeds and peanut butter. Rodents were euthanized in a carbon dioxide © Cambridge University Press 2017 (CO2) chamber, necropsied and the gastrointestinal tracts were analysed under a stereomicro- scope for the presence of parasites. Cestodes were washed in 0.65% saline solution and fixed in hot AFA (70°GL ethanol, 93%; formaldehyde, 5%; glacial acetic acid, 2%) (70°C) overnight, conserved in 70% ethanol and stained by the regressive process with Grenacher’s borax car- mine, dehydrated in an increasing series of alcohol, according to Amato et al. (1991), with Downloaded from https://www.cambridge.org/core. IP address: 191.178.36.221, on 05 Oct 2017 at 09:53:29, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0022149X17000773 2 L.C. Oliveira et al. modifications as follows. Specimens were cleared in clove oil and (2419–2960, n = 5) at level of gravid proglottids. Scolex rounded, mounted in Dammar gum. The scolices were mounted in Hoyer’s wider than long, 386 ± 95.51 (256–547, n = 6) wide by 334 ± 67.79 medium for counting and measurement of the hooks, according (254–454, n = 6) long, not clearly distinct from neck (fig. 1a). to Ganzorig et al.(1999). Two scolices were macerated in a tiny Retracted rostellum, measuring 23 ± 3.58 (17–29, n = 6) long by mortar and pestle with a little distilled water for better visualiza- 64 ± 8.17 (53–78, n = 6) wide; with rounded apex, armed with tion of the morphology of the spines. The macerate was placed double crown of hammer-shaped hooks (fig. 1d), similar in between a slide and cover slip and observed on a light microscope. length. Hooks 102 ± 15.8 (89–128, n = 6) in number, measuring Mature proglottids were embedded in paraffin and serial cross- 10 ± 0.89 (9–13, n = 15) long. Four rounded muscular suckers, sections were cut at a thickness of 5 μm. Sections were stained 80 ± 6.94 (63–92, n = 14) of greatest diameter by 63 ± 7.33 (48– with haematoxylin and eosin and mounted in Canada balsam. 73, n = 14) of smaller diameter, armed with four rows of hooks The morphometric measurements were taken from the stained (figs 1a, 2). cestode specimens. All measurements are given in micrometres Neck unclearly distinct from scolex, 275 ± 68.22 (205–399, n = unless otherwise stated. Measurements were taken with an 6) wide. First evidence of segmentation 4094 ± 751.8 (3032–5347, Axioplan Zeiss light microscope (Carl Zeiss, Jena, Germany) n = 6) from scolex apex. Mature proglottids (fig. 1b) measuring equipped with a Canon Power-Shot A640 digital camera 348 ± 33.0 (295–381, n = 8) long by 1459 ± 66.6 (1373–1552, (Canon, Beijing, China) and Zeiss AxioVision Sample Images n = 8) wide. Genital pores irregularly alternating, open at anterior Software for image analysis. The drawings were made using a third of lateral proglottid margin (fig. 1b, c). Genital atrium shal- camera lucida. The illustrations were drawn with Intuos4 low (fig. 1e), 29 ± 8.3 (16–40, n = 12) in diameter. Wacon® pen tablet (Wacom, Beijing, China) using Adobe Testes approximately spherical, measuring 53 ± 6.30 (32–59, Photoshop Elements 8.0. n = 30) at greatest diameter by 46 ± 6.45 (27–57, n = 30) at smaller For scanning electron microscopy, cestodes were fixed for 2 h diameter. Number of testes per proglottid about 28 ± 3.18 (22–32, in 2.5% glutaraldehyde, 4% freshly prepared paraformaldehyde, n = 12), arranged in two groups 12 ± 1.1 (10–13, n = 12) on the 5mM calcium chloride in 0.1 M cacodylate buffer, pH 7.2 and poral side and 17 ± 3 (12–21, n = 12) on the aporal side, distribu- post-fixed in 2% osmium tetroxide in 0.1 M cacodylate buffer, ted between the osmoregulatory canals (fig. 1b). Cirrus pouch according to Ederli et al. (2008). The samples were dehydrated pear-shaped, 146 ± 12.3 (123–170, n = 11) long and 76 ± 4.6 – in an acetone series, critical-point dried with CO2, sputter-coated (65 79, n = 12) wide. Short internal seminal vesicle, slightly coiled with gold and examined in a Zeiss EVO 40 scanning electron (fig. 1e). Vas deferens narrow and coiled, passing between dorsal microscope (SEM) operating at 15 kV. and ventral osmoregulatory canals, extending to the base of cirrus Slides deposited at the Helminthological Collection of the pouch (fig. 1b, g). Oswaldo Cruz Institute (CHIOC), Fundação Oswaldo Cruz Female genital complex median (fig. 1b). Ovary multilobed, (FIOCRUZ), Rio de Janeiro, Brazil, under the registration num- bi-alate, with length 245 ± 39.5 (172–297, n = 12) and maximum bers CHIOC 33.043 A–C, CHIOC 33.043 D–F and CHIOC width 404 ± 42.4 (313–446, n = 12). Vitellarium compact, poster- 33.044, collected from Oryzomys ratticeps from Paraná State, ior to ovary, disposed slightly antiporally to ovary; measuring 184 Brazil and identified as (Raillietina) Raillietina guaricana, were ± 19.4 (156–226, n = 12) long by 159 ± 9.1 (146–180, n = 12) wide used for comparison. (fig. 1b). Vagina opening and passing ventrally and posteriorly to cirrus pouch. Gravid proglottids wider than long, measuring 753 ± 66.6 Results (649–895, n = 28) long by 2754 ± 141 (2419–2960, n = 28) wide, – Fuhrmannetta jurubatensis n. sp. containing 56 ± 4.2 (50 69, n = 28) uterine egg capsules, 141 ± 6.4 (131–154, n = 14) long by 168 ± 10 (154–187, n = 14) wide, Taxonomic summary situated between ventral osmoregulatory canals (fig. 1c). Terminal gravid proglottids longer than wide, measuring 1575 Type host. Cerradomys goytaca (Tavares, Pessôa & Gonçalves, ± 101.3 (1366–1689, n = 15) long by 1139 ± 162 (930–1364, n = 2011) (Rodentia, Cricetidae). 15) wide, containing 64 ± 6.5 (53–76, n = 15) uterine egg capsules, Type locality. Reserva de Jurubatiba, Quissamã, Rio de Janeiro, 209 ± 18.6 (173–243, n = 14) long by 174 ± 23.1 (116–202, n = 14) Brazil (22°10′36.5′′S, 41°25′42.0′′W).