CURRICULUM VITAE JOHN A. GERLT Personal Date of Birth: July 28, 1947 Place of Birth: Sycamore, Illinois Home Address: 1805 Byrnebruk Drive Champaign, IL 61822 USA Office Address: Department of Biochemistry University of Illinois 600 South Mathews Avenue Urbana, IL 61801 USA Education B.S. (1969) Michigan State University A.M. (1970) Harvard University Ph.D. (1974) Harvard University Positions Held Jane Coffin Childs Memorial Fund Fellow, NIH, 1974-75 Assistant Professor of Chemistry, Yale University, 1975-81 Associate Professor of Chemistry, Yale University, 1981-84 Professor of Chemistry and Biochemistry, University of Maryland, 1984-1994 Professor of Biochemistry, University of Illinois, Urbana-Champaign, 1994-present Professor of Chemistry, University of Illinois, Urbana-Champaign, 1994-present Head of Biochemistry, University of Illinois, Urbana-Champaign, 1994-2004 Gutgsell Chair, University of Illinois, 2004-present Awards and Honors Jane Coffin Childs Memorial Fund Fellowship, 1974-75 NIH Research Career Development Award, 1978-83 Alfred P. Sloan Foundation Fellowship, 1981-85 Arthur C. Cope Travel Award, American Chemical Society, 1982 Co-Chair, Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, 1984 Member, Physical Biochemistry Study Section, NIH, 1986-90 Sigma Xi Award for Contribution to Science, University of Maryland Chapter, 1989 University of Maryland College Park, Distinguished Faculty Research Fellowship, 1990-91 Repligen Corporation Award in Chemistry of Biological Processes, 2003 Fellow, American Association for the Advancement of Science (AAAS), 2007 2 Advisory Panel, Schwerpunkt on Directed Evolution, Deutsche Forschung Gemeinschaft, 2004-2009 Arthur C. Cope Scholar Award, American Chemical Society, 2010 A. I. Scott Medal, Department of Chemistry, Texas A&M University, 2010 Associate Editor, Biochemistry, 2004-present Research Support Structural Bases for Catalytic Efficiency and Substrate Specificity. NIH R01 GM065155, 2/1/03 – 8/30/12. Includes contracts to the Albert Einstein College of Medicine (S. Almo) and University of California, San Francisco (M.P. Jacobson). Current year: total costs (including subcontract), $350,000; Illinois direct costs, $170,000. Deciphering Enzyme Specificity. NIH P01 GM071790, 7/10/04 – 6/30/14. Includes subcontracts to Texas A&M University (F. Raushel), University of California, San Francisco (P. Babbitt, A. Sali, B. Shoichet, and M. Jacobson) and Albert Einstein College of Medicine (S. Almo). Current year (7/10/09-6/30/10): total costs (including subcontracts), $1,540,710; Illinois direct costs, $240,000. Planning Grant for Collaborative Project for an Enzyme Function Initiative, NIH 1R24 GM087999-01, 4/10/09 – 3/30/11. Direct costs, $50,000. A Collaborative Center for an Enzyme Function Initiative. NIH U54 GM093342, 5/01/10 — 4/30/15/ A Glue Grant that includes J. A. Gerlt (PI) and thirteen CoPIs: Steven Almo (Albert Einstein College of Medicine), Karen Allen (Boston University), Richard Armstrong (Vanderbilt University Medical Center), Patricia Babbitt (UCSF), John Cronan (UIUC), Debra Dunaway-Mariano (University of New Mexico), Matthew Jacobson (UCSF), Wladek Minor (University of Virgina), C. Dale Poulter (University of Utah), Frank Raushel (Texas A&M University), Andrej Sali (UCSF), Brian Shoichet (UCSF), and Jonathan V. Sweedler (UIUC). Current year (5/01/10-4/30/11): total costs (including subcontracts), $4,500,000; Illinois direct costs, $1,100,000 (including Administrative Core, Microbiology Core, and Enolase Superfamily Bridging Project). Biochemistry, Structure, and Engineering of Enzymes to Overcome Biomass Recalcitrance, Energy Biosciences Institute, 1/1/08-12/31/12. Current year: Direct costs, $500,000. This project includes John E. Cronan and Satish K. Nair, CoPIs. Publications The Mechanism of Action of 5'-Adenylic Acid-Activated Threonine Dehydrase. IV. Characterization of the Kinetic Effect of Adenosine Monophosphate. C. P. Dunne, J. A. GERLT, K. W. Rabinowitz, and W. A. Wood, J. Biol.Chem. 1973, 248, 8189. The Mechanism of Action of 5'-Adenylic Acid Activated Threonine Dehydrase. V. Relation between Ligand-Induced Allosteric Activation and the Protomer-Oligomer Interconversion, J. A. GERLT, K. W. Rabinowitz, C. P. Dunne, and W. A. Wood, J. Biol. Chem. 1973, 248, 8200. 3 A Phosphohydrolase from Enterobacter aerogenes, J. A. GERLT and F. H. Westheimer, J. Am. Chem. Soc. 1973, 95, 8166. Enthalpy of Hydrolysis of Simple Phosphate Esters, J. M. Sturtevant, J. A. GERLT, and F. H. Westheimer, J. Am. Chem. Soc. 1973, 95, 8168. Regulation of L-Threonine Dehydrase by Ligand-Induced Oligomerization, C. P. Dunne, R. C. Menson, J. A. GERLT, and W. A. Wood, in "Metabolic Interconversion of Enzymes," E. H. Fischer et al. (Eds.), Springer-Verlag, New York, 1974, pp. 349. Purification and Properties of a Phosphohydrolase from Enterobacter aerogenes, J. A. GERLT and G. J. R. Whitman, J. Biol. Chem. 1975, 250, 5053. The Enthalpies of Hydrolysis of Acyclic, Monocyclic, and Glycoside Cyclic Phosphate Diesters, J. A. GERLT, F. H. Westheimer, and J. M. Sturtevant, J. Biol. Chem. 1975, 250, 5059. Stereochemistry of the Hydrolysis of the Endo Isomer of Uridine 2',3'-Cyclic Phosphorothioate Catalyzed by the Nonspecific Phosphoydrolase from Enterobacter aerogenes, J. A. GERLT and W. H. Y. Wan, Biochemistry 1979, 18, 4630. Thermochemical Identification of the Structural Factors Responsible for the Thermodynamic Instability of 3',5'-Cyclic Nucleotides, J. A. GERLT, N. I. Gutterson, P. Datta, B.Belleau, and C. L. Penney, J. Am. Chem. Soc. 1980, 102, 1655. Theoretical Calculations on the Geometric Destabilization of 3',5'- and 2',3'-Cyclic Nucleotides, F. J. Marsh, P. Weiner, J. E. Douglas, P. A. Kollman, G. L. Kenyon, and J. A. GERLT, J. Am. Chem. Soc. 1980, 102, 1660. Conformational Properties of Substituted Trimethylene Phosphates in Solution, J. A. GERLT, N. I. Gutterson, R. E. Drews, and J. A. Sokolow, J. Am. Chem. Soc. 1980, 102, 1665. Crystal and Molecular Structure of 5-Ethoxy Trimethylene Phosphoric Acid, J. A. GERLT, D. F. Chodosh, R. E. Drews, and R. D. Adams, J. Org. Chem. 1980, 45, 1282. Mechanism of the Adenylate Cyclase Reaction. Stereochemistry of the Reaction Catalyzed by the Enzyme from Brevibacterium liquefaciens, J. A. GERLT, J. A. Coderre, and M. S. Wolin, J. Biol. Chem. 1980, 255, 331. Oxygen Chiral Phosphodiesters. 1. Synthesis and Configurational Analysis of Cyclic [18O]-2'-Deoxyadenosine 3',5'-Monophosphate, J. A. GERLT and J. A. Coderre, J. Am. Chem. Soc. 1980, 102, 4531. 4 Syntheses and Configurational Assignments of the Diastereomers of the 4- Nitrophenyl Esters of Thymidine 3'-(N-Phenyl Phosphoramidate) and Thymidine 5'- (N-Phenyl Phosphoramidate), J. A. GERLT, S. Mehdi, J. A. Coderre, and W. O. Rogers, Tet. Letters 1980, 21, 2385. Oxygen Chiral Phosphodiesters. 2. Enzymatic Synthesis and Configurational Analysis of [-18O]-2'-Deoxyadenosine 5'-Diphosphate, J. A. Coderre and J. A. GERLT, J. Am. Chem. Soc. 1980, 102, 6594. The Solution Conformational Preferences of the Sugar and Sugar Phosphate Constituents of RNA and DNA, J. A. GERLT and A. V. Youngblood, J. Am. Chem. Soc. 1980, 102, 7433. Oxygen Chiral Phosphodiesters. 3. Use of 17O NMR to Demonstrate Configurational Differences in the Diastereomers of Cyclic [17O,18O]-2'- Deoxyadenosine 3',5'-Monophosphate, J. A. Coderre, S. Mehdi, P. C. Demou, R. Weber, D. D. Traficante, and J. A. GERLT, J. Am. Chem. Soc. 1981, 103, 1870. Oxygen Chiral Phosphodiesters. 4. Stereochemical Course of the Hydrolysis of Cyclic [17O,18O]-2'-Deoxyadenosine 3',5'-Monophosphate in H216O Catalyzed by Bovine Heart Cyclic Nucleotide Phosphodiesterase, J. A. Coderre, S. Mehdi, and J. A. GERLT, J. Am. Chem. Soc. 1981, 103, 1872. Oxygen Chiral Phosphodiesters. 5. Stereochemical Course of the Hydrolysis of Thymidine 3'-(4-Nitrophenyl [17O,18O]Phosphate) in H216O Catalyzed by the Exonuclease from Bovine Spleen, S. Mehdi and J. A. GERLT, J. Am. Chem. Soc. 1981, 103, 7018. Stereochemical Course of the Hydrolysis of Thymidine 5'-(4-Nitrophenyl [17O,18O]Phosphate) in H216O Catalyzed by the Phosphodiesterase from Snake Venom, S. Mehdi and J. A. GERLT, J. Biol. Chem. 1981, 256, 12164. High Field 17O NMR Studies of Adenine Nucleotides, J. A. GERLT, P. C. Demou and S. Mehdi, Nucleic Acids Research Symposium Series No. 9 1981, 11. Syntheses and Configurational Assignments of Thymidine 3'- and 5'-(4-Nitrophenyl [17O,18O]Phosphates), S. Mehdi, J. A. Coderre, and J. A. GERLT, ACS Symposium Series 1981, 171, 109. 17O NMR Spectral Properties of Simple Phosphate Esters and Adenine Nucleotides, J. A. GERLT, P. C. Demou, and S. Mehdi, J. Am. Chem. Soc. 1982, 104, 2848. Oxygen Chiral Phosphodiesters. 7. Stereochemical Course of a Reaction Catalyzed by Staphylococcal Nuclease, S. Mehdi and J. A. GERLT, J. Am. Chem. Soc. 1982, 104, 3223. 17O NMR Spectral Properties of Pyrophosphate, Simple Phosphonates, and Thiophosphate and Phosphonate Analogs of ATP, J. A. GERLT, M. A. Reynolds, P. C. Demou, and G. L. Kenyon, J. Am. Chem. Soc. 1983, 105, 6469. 5 15N and 17O Studies of the Proton Binding Sites in Imidodiphosphate, Tetraethyl Imidodiphosphate, and Adenylyl Imidodiphosphate, M. A. Reynolds, J. A. GERLT, P. C. Demou, N. J. Oppenheimer, and G. L. Kenyon, J. Am. Chem. Soc. 1983, 105, 6475. Oxygen Chiral Phosphodiesters. 8. Stereochemical Course of the Base-Catalyzed Hydrolysis of Cyclic [17O,18O]-2'-Deoxyadenosine 3',5'-Monophosphate, S. Mehdi, J. A. Coderre, and J. A. GERLT, Tetrahedron 1983, 39, 3448. 17O-NMR as a Probe of Nucleic