Centre for Genomic Regulation Annual Report 2009 © Copyright 2010 Produced by: Department of Communication & Public Relations Centre for Genomic Regulation (CRG) Dr. Aiguader, 88 08003 Barcelona, Spain www.crg.es Texts and graphics: CRG Researchers, Department of Communication and Public Relations Graphic Design: Genoma ArtStudio SCP (www.genoma-artstudio.com) Photography: Ivan Paulick, Sergi Espada Printing: Novoprint, S.A. Legal deposit: B-31.812-2010 CONTENTS CRG Scientific Structure 6 CRG Core Facilities Structure 8 CRG Management Structure 10 CRG Scientific Advisory Board (SAB) 12 CRG Business Board 13 Year Retrospect by the Director of the CRG: Miguel Beato 14 Research Programmes Gene Regulation 16 > Chromatin and gene expression 18 > Regulation of alternative pre-mRNA splicing during cell 22 differentiation, development and disease > RNA interference and chromatin regulation 26 > RNA-protein interactions and regulation 29 > Regulation of protein synthesis in eukaryotes 33 > Translational control of gene expression 36 Differentiation and Cancer 42 > Hematopoietic differentiation and stem cell biology 44 > Epigenetics events in cancer 48 > Epithelial homeostasis and cancer 52 > Mechanisms of cancer and ageing 55 Genes and Disease 58 > Genetic causes of disease 60 > Gene therapy 69 > Gene function and murine models of disease 73 > Neurobehavioral phenotyping of mouse models of disease 77 Bioinformatics and Genomics 82 > Bioinformatics and genomics 84 > Comparative bioinformatics 94 > Comparative genomics 98 > Evolutionary genomics 103 Cell and Developmental Biology 108 > Intracellular compartmentation 110 > Microtubule function and cell division 114 > Sensory cell biology and organogenesis 118 > Coordination of cytokinesis with chromosome segregation 122 > Biomechanics of morphogenesis 125 128 Systems Biology 130 > Design of biological systems 136 > Systems analysis of development 140 > Gene network engineering 145 > Genetic systems 149 > Sensory systems and behaviour 152 > Comparative analysis of developmental systems 156 Core Facilities 159 > Genomics Unit 159 Genotyping Unit 165 Ultrasequencing Unit 168 Microarrays Unit 172 > Proteomics Unit 175 > Advanced Light Microscopy Unit 179 > High-Throughput Screening Unit 182 > Bioinformatics Unit 186 Highlights: 187 > Gene Regulation Programme: “Luxury Packages For Genome Messages” 188 > Differentiation and Cancer Programme: “A Step Forward In Cell Reprogramming” 189 > Genes and Disease Programme: “Genetic Causes For Psoriasis” 190 > Bionformatics and Genomics Programme: “The Bovine Genome: New Information About Mammalian Evolution And Clues To Efficient Cattle” 191 > Cell and Developmental Biology Programme: “Tango1: A Guide For Protein Transport Inside The Cell” 192 > Systems Biology Programme: “Even The Simplest Form Of Life Is More Complex Than Expected” 194 Appendix 1: > VIII CRG Annual Symposium: “Stem Cells, Differentiation and Cancer” 196 Appendix 2: > PRBB-CRG Sessions and Programme & Core Facilities Seminars 202 Appendix 3: > Grants 203 Appendix 4: > Finance and Personnel Evolution 206 Appendix 5: > Press Clipping SCIENTIFIC STRUCTURE BOARD OF TRUSTEES DIRECTOR Miguel Beato ........................................................................................ DEPUTY DIRECTOR Luis Serrano SCIENTIFIC FACULTY BOARD SCIENTIFIC BUSINESS BOARD ADVISORY BOARD EXECUTIVE BOARD COMMITTEE GENE BIOINFORMATICS CELL AND SYSTEMS DIFFERENTIATION GENES REGULATION AND GENOMICS DEV. BIOLOGY BIOLOGY AND CANCER AND DISEASE MIGUEL BEATO RODERIC GUIGÓ VIVEK MALHOTRA LUIS SERRANO THOMAS GRAF XAVIER ESTIVILL Coordinator Coordinator Coordinator Coordinator Coordinator Coordinator INTRACELLULAR HEMATOPOIETIC CHROMATIN AND BIOINFORMATICS DESIGN OF COMPARTMENTATION DIFFERENTIATION AND GENE EXPRESSION AND GENOMICS BIOLOGICAL SYSTEMS GENETIC CAUSES STEM CELL BIOLOGY OF DISEASE RODERIC GUIGÓ VIVEK MALHOTRA LUIS SERRANO MIGUEL BEATO Group Leader THOMAS GRAF Group Leader Group Leader Group Leader XAVIER ESTIVILL Group Leader Group Leader ............................... FUNCTIONAL GENOMICS OF NEUROLOGICAL REGULATION MICROTUBULES COMPARATIVE SYSTEMS ANALYSIS EPIGENETICS EVENTS DISORDERS OF ALTERNATIVE FUNCTION AND CELL BIOINFORMATICS OF DEVELOPMENT IN CANCER PRE-MRNA SPLICING DIVISION EULÀLIA MARTÍ CEDRIC NOTREDAME JAMES SHARPE LUCIANO DI CROCE Staff Scientist JUAN VALCÁRCEL Group Leader ISABELLE VERNOS Group Leader Group Leader Group Leader Group Leader EPITHELIAL RNA INTERFERENCE COMPARATIVE GENE NETWORK SENSORY CELL BIOLOGY HOMEOSTASIS GENE THERAPY AND CHROMATIN GENOMICS AND ORGANOGENESIS ENGINEERING AND CANCER REGULATION TONI GABALDÓN MARK ISALAN CRISTINA FILLAT HERNAN LOPEZ-SCHIER SALVADOR Group Leader RAMIN SHIEKHATTAR Group Leader Group Leader Group Leader Group Leader AZNAR-BENITAH Group Leader NEUROBEHAVIORAL TRANSLATIONAL CYTOKINESIS AND PHENOTYPING OF CONTROL OF GENE EVOLUTIONARY GENETIC SYSTEMS MECHANISMS OF CHROMOSOME SEGRE- MOUSE MODELS EXPRESSION GENOMICS CANCER AND AGING GATION BEN LEHNER OF DISEASE FYODOR KONDRASHOV Group Leader BILL KEYES RAÚL MÉNDEZ MANUEL MENDOZA Group Leader Group Leader Group Leader MARA DIERSSEN Group Leader Group Leader GENE FUNCTION REGULATION OF BIOMECHANICS AND MURINE MODELS PROTEIN SYNTHESIS SENSORY SYSTEMS OF MORPHOGENESIS OF DISEASE IN EUKARYOTES AND BEHAVIOUR JÉRÔME SOLON SUSANA DE LA LUNA FÁTIMA GEBAUER Group Leader MATTHIEU LOUIS Group Leader Group Leader MARIONA ARBONÉS Group Leaders RNA-PROTEIN COMPARATIVE INTERACTIONS ANALYSIS OF AND REGULATION DEVELOPMENTAL SYSTEMS JOSEP VILARDELL Group Leader JOHANNES JÄGER Group Leader CORE FACILITIES STRUCTURE BOARD OF TRUSTEES DIRECTOR CEO Miguel Beato Marian Marrodan CORE FACILITIES Doris Meder Director GENOMICS UNIT PROTEOMICS FACS UNIT ADVANCED LIGHT BIOINFORMATICS HTS/ROBOTICS UNIT (CRG/UPF) (CRG/UPF) MICROSCOPY UNIT UNIT UNIT (CRG/UPF) GENOTYPING UNIT HENRIK MOLINA ÒSCAR FORNAS (UPF) GUGLIELMO ROMA RAÚL GÓMEZ Unit Leader Unit Leader TIMO ZIMMERMANN Unit Leader Unit Responsible MÒNICA BAYÉS Unit Leader Unit Leader ............................... MICROARRAYS UNIT MÒNICA BAYÉS Unit Leader ............................... ULTRASEQUEN- CING UNIT HEINZ HIMMELBAUER Unit Leader . Annual Report 2009 .9 MANAGEMENT STRUCTURE BOARD OF TRUSTEES DIRECTOR ASSISTANT DIRECTOR CEO CEO ASSISTANT Reyes Perza Miguel Beato Marian Marrodán Ester Montava HUMAN FINANCES AND COMMUNICATION RESOURCES / GENERAL TECHNOLOGY PROGRAMMES/CF GRANTS RESEARCH ICT AND PUBLIC OFFICE LEGAL SERVICES TRANSFER & RECEPTION EVA DEL PINTO DAVID CAMARGO RELATIONS SECRETARIAT BRUNA VIVES DEPARTMENT JOSEP QUERALT XAVIER RÚBIES GLORIA LLIGADAS ESTER MONTAVA CRISTINA CASAUS EUROPEAN & HR-PERSONNEL INTERNATIONAL PRESS OFFICER PROGRAMME MANAGEMENT ENGINEERING FUNDING SUPPORT SERVICE & EVENTS PROJECT MANAGER & CORE FACILITIES SUPPORT ORGANISATION ISMAEL DE MINGO GLORIA FREIXAS SILVIA TÓRTOLA SECRETARIAT BRUNA VIVES JORDI PAIRÓ MARC GONZÁLEZ LAIA CENDRÓS ACCOUNTING CLARA LÓPEZ DIEGO MELLIBOVSKY MAGALÍ BARTOMEUS ENRIC GUMMÀ GEMMA PÉREZ MARÍA ROZAS IMMA FALERO ANNA PAYTUVÍ BLANKA WYSOCKA VERÓNICA JAREÑO NATIONAL FUNDING SCIENCE RUT CARBONELL SYSTEMS HR-FELLOWSHIP DISSEMINATION ARANTXA FERRER ADMINISTRATION AND STUDENTS ROMINA GARRIDO MANAGEMENT ANNICK LABEEUW MARTA SALLÉS OUTSOURCING PURCHASING ALBA SHAW NURIA JANÉ AND LOGISTICS MARC GONZÁLEZ SERGI REPULLO MIREIA PÉREZ MONTSERRAT ESTAPÉ CORE FACILITIES ORACLE LEGAL DAVID RODRÍGUEZ RECEPTION ADMINISTRATION DEVELOPMENT DEPARTMENT JUAN CARLOS GÓMEZ AREA MARTA CASALS JAUME BACARDIT CRISTINA CASAUS VÍCTOR ELÍAS ANA CELAYA CONTROL WEB SCIENTIFIC AND PROJECTS DEVELOPMENT HR SUPPORT EQUIPMENTS JUSTIFICATON AREA BÁRBARA FERREIRA NICOLA COLLU DAVID CASTILLO GEMMA SALVADÓ MARIANA MORLANS STEFAN PONISCH ENRIC GUMMÀ EVA DEL PINTO JOAN CONANGLA MAINTENANCE OLGA JUDERÍAS JOSEP QUERALT WORKING RISKS / RADIOACTIVITY / WASTES SONIA ALCÁZAR MªJOSÉ ROMA EiPMC ARANTXA VENTURA ELISABETH MUÑOZ MARTA POU SCIENTIFIC ADVISORY BOARD (SAB) Chairman Dr. Kai Simons Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany Members Dr. Stylianos Emmanuel Antonarakis Medical Genetics, University of Geneva, Geneva, Switzerland Dr. Michael Ashburner European Bioinformatics Institute-EMBL Outstation, Department of Genetics, University of Cambridge, Cambridge, United Kingdom Dr. Pierre Chambon Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Strasbourg, France Dr. Iain Mattaj European Molecular Biology Laboratory (EMBL), Heidelberg, Germany Dr. Joan Modolell Center of Molecular Biology “Severo Ochoa”, CSIC & Autonomous University of Madrid, Madrid, Spain Dr. Arnold Munnich Det. Génétique, Hôpital des Enfants Malades, Paris, France Dr. Christiane Nüsslein-Volhard Abt. Genetik, Max Planck Institut für Entwicklungsbiologie, Tübingen, Germany Dr. Marc Vidal Dana Farber Cancer Institute, Boston, USA Dr. Erwin Wagner Spanish National Cancer Research Centre, Madrid, Spain 12 . Annual Report 2009 . BUSINESS BOARD President Dr. Josep M. Taboada Medical Director, Sanofi-Aventis Vice-president Dr. Antonio Parente President, Grup Lipotec Members Dr. Pere Berga R+D Management Director, Almirall Prodesfarma, S.A. Dr. Clara Campàs Executive Vice-President, Advancell Therapeutics Dr. Antoni Esteve President, Esteve Dr. Josep Prous, Jr. Executive Vice President, Prous Science Sr. Jordi Ramentol General Manager, Grupo Ferrer Internacional, S.A. Mr. Josep Lluís Sanfeliu Co-founder partner, Ysios Capital Partners Sr. Fernando Turró General Manager, Contratas y Obras . Annual Report 2009 .13 YEAR RETROSPECT by the Director