Figure. S1 The quantities in the chosen columns vs. the soft threshold power Figure S2. Heat map of differentially expressed genes. X-axis shows the cluster of cases (cluster 1) and controls (cluster 2), Y-axis shows the dendrogram of differentially expressed genes. Genes 2018, 9, x; doi: FOR PEER REVIEW www.mdpi.com/journal/genes Genes 2018, 9, x FOR PEER REVIEW 2 of 39 Figure. S3 a. Expression level of SLC32A1 between cases and controls Genes 2018, 9, x FOR PEER REVIEW 3 of 39 b. Expression level of HINT1 between cases and controls Genes 2018, 9, x FOR PEER REVIEW 4 of 39 c. Expression level of IFITM3 between cases and controls Genes 2018, 9, x FOR PEER REVIEW 5 of 39 d. Expression level of RELN between cases and controls Genes 2018, 9, x FOR PEER REVIEW 6 of 39 Figure. S4 Protein-Protein Interaction Network structure of detected modules a. Module yellow Genes 2018, 9, x FOR PEER REVIEW 7 of 39 b. Module turquoise Genes 2018, 9, x FOR PEER REVIEW 8 of 39 c. Module blue Genes 2018, 9, x FOR PEER REVIEW 9 of 39 d. Module brown Genes 2018, 9, x FOR PEER REVIEW 10 of 39 Table S1. Sample demographics Sex (% N Age pH PMI RIN Subtype MoodDx Substance Dx Suicide Antidepr Tox F) 7 BN 2 AN-BN ED 15 86.7 40.1 ±8.1 6.2 ±0.2 32.3 ±19.5 7.57 ±0.88 86.70% 53.30% 73.30% 66.70% 3 AN 3 NOS Control 102 20.5 42.5 ±17.2 6.6±0.3 27.2±14.8 8.3±0.69 Abbreviations: AN, anorexia nervosa (restricting); AN-BN, anorexia nervosa, binge–purge type; AntidepressTox, percentage of cases on antidepressants at the time of death as measured by blood/brain toxicology; BN, bulimia nervosa; ED, eating disorder; MoodDx, presence of comorbid Axis I mood disorder diagnosis; NOS, eating disorder not otherwise specified; pH, brain pH; PMI, postmortem interval in hours; RIN, RNA integrity number; Substance Dx, presence of comorbid Axis I substance use disorder. All numerical cells are mean±s.d. Genes 2018, 9, x FOR PEER REVIEW 11 of 39 Table S2. Differentially expressed genes Expression genname logFC AveExpr t P.Value adj.P.Val B TNFRSF6B 0.81 8.01 6.15 1.07E-08 1.42E-04 9.47 TSHZ2 0.47 8.69 5.44 2.84E-07 9.35E-04 6.48 AP1G2 0.45 8.50 5.16 1.01E-06 1.37E-03 5.33 HYOU1 0.49 9.08 5.15 1.05E-06 1.37E-03 5.29 ELK1 0.53 9.76 5.13 1.14E-06 1.37E-03 5.21 CHTF18 0.27 7.74 5.11 1.23E-06 1.37E-03 5.15 IGSF9B 0.41 8.00 5.07 1.49E-06 1.52E-03 4.97 COL4A1 0.59 8.28 5.02 1.80E-06 1.58E-03 4.80 SIN3B 0.51 8.86 4.78 4.97E-06 2.58E-03 3.88 PPP1R10 0.38 8.40 4.77 5.21E-06 2.58E-03 3.83 ULK3 0.35 8.11 4.77 5.26E-06 2.58E-03 3.83 DGCR2 0.47 9.63 4.71 6.78E-06 3.21E-03 3.60 ABCA7 0.37 7.91 4.67 7.84E-06 3.26E-03 3.46 TTC14 0.46 9.21 4.67 8.02E-06 3.26E-03 3.44 LOC146177 0.26 7.76 4.66 8.15E-06 3.26E-03 3.43 Up-regulation ST6GALNAC6 0.58 10.51 4.55 1.31E-05 4.69E-03 3.00 PTCHD1 0.34 8.69 4.51 1.56E-05 5.27E-03 2.84 SRC 0.40 9.07 4.49 1.64E-05 5.28E-03 2.80 PARC 0.33 8.07 4.44 2.03E-05 5.78E-03 2.61 CSDA 0.78 8.90 4.44 2.06E-05 5.78E-03 2.59 SFRS14 0.34 8.30 4.42 2.20E-05 5.78E-03 2.53 LOC654342 0.34 7.85 4.42 2.23E-05 5.78E-03 2.52 CHMP1A 0.40 9.21 4.37 2.64E-05 6.36E-03 2.37 HS.490981 0.40 8.38 4.37 2.65E-05 6.36E-03 2.36 DGKG 0.27 7.80 4.36 2.73E-05 6.36E-03 2.34 LAMC1 0.33 8.99 4.36 2.77E-05 6.36E-03 2.33 MTHFR 0.29 7.93 4.36 2.79E-05 6.36E-03 2.32 ZNF562 0.27 8.59 4.33 3.12E-05 6.89E-03 2.22 CACNA1A 0.55 8.29 4.33 3.13E-05 6.89E-03 2.22 GADD45B 0.56 7.92 4.29 3.65E-05 7.81E-03 2.08 HELZ 0.30 8.96 4.29 3.71E-05 7.81E-03 2.06 Genes 2018, 9, x FOR PEER REVIEW 12 of 39 CHERP 0.22 8.03 4.29 3.72E-05 7.81E-03 2.06 LOC644931 0.51 8.44 4.27 4.03E-05 8.07E-03 1.99 SHC1 0.42 9.14 4.26 4.13E-05 8.07E-03 1.97 HS.388347 0.32 8.26 4.26 4.19E-05 8.07E-03 1.95 IFT140 0.26 7.85 4.25 4.21E-05 8.07E-03 1.95 CACNA1C 0.26 8.04 4.22 4.82E-05 8.52E-03 1.83 ZER1 0.38 8.73 4.21 4.92E-05 8.52E-03 1.81 GNAO1 0.63 11.80 4.21 5.05E-05 8.52E-03 1.78 ADCY6 0.25 8.03 4.20 5.24E-05 8.52E-03 1.75 NPIP 0.47 10.30 4.19 5.35E-05 8.52E-03 1.73 RNF40 0.27 8.56 4.16 6.09E-05 9.37E-03 1.62 ANKRD20A1 0.62 10.26 4.15 6.19E-05 9.42E-03 1.60 BAG4 0.35 8.07 4.11 7.20E-05 1.05E-02 1.47 EXTL3 0.44 9.57 4.08 8.30E-05 1.16E-02 1.34 PHYHIP 0.57 9.27 4.08 8.34E-05 1.16E-02 1.33 RGS11 0.32 8.07 4.05 9.01E-05 1.23E-02 1.26 GNB2 0.27 7.94 4.05 9.17E-05 1.24E-02 1.25 TUBGCP6 0.20 7.77 4.05 9.25E-05 1.24E-02 1.24 SLC38A11 0.34 8.28 4.03 9.88E-05 1.27E-02 1.18 OGDH 0.44 9.07 4.02 1.04E-04 1.29E-02 1.14 FURIN 0.18 7.75 4.01 1.05E-04 1.29E-02 1.13 NARFL 0.27 8.30 4.00 1.11E-04 1.33E-02 1.07 ADAM11 0.41 8.99 3.99 1.14E-04 1.35E-02 1.05 HS.34558 0.26 8.20 3.99 1.15E-04 1.35E-02 1.04 CEBPD 0.66 9.28 3.98 1.19E-04 1.35E-02 1.02 HS.181500 0.31 8.32 3.98 1.20E-04 1.35E-02 1.00 MT1M 0.86 9.40 3.98 1.21E-04 1.35E-02 1.00 RSAD1 0.27 7.90 3.97 1.22E-04 1.35E-02 0.99 CELSR3 0.38 9.92 3.97 1.23E-04 1.35E-02 0.99 BZRAP1 0.44 9.50 3.97 1.24E-04 1.35E-02 0.98 LOC729008 0.23 7.71 3.97 1.25E-04 1.35E-02 0.97 ST5 0.26 8.01 3.96 1.27E-04 1.36E-02 0.96 SLC9A5 0.26 7.91 3.95 1.34E-04 1.37E-02 0.91 Genes 2018, 9, x FOR PEER REVIEW 13 of 39 COLQ 0.21 8.06 3.95 1.35E-04 1.37E-02 0.90 MBOAT7 0.45 9.48 3.94 1.36E-04 1.37E-02 0.90 SMARCD3 0.27 8.51 3.94 1.36E-04 1.37E-02 0.90 B4GALNT4 0.47 8.95 3.94 1.37E-04 1.38E-02 0.89 ANKFY1 0.21 8.07 3.94 1.39E-04 1.38E-02 0.88 CIC 0.28 8.36 3.94 1.40E-04 1.38E-02 0.87 LOC728734 0.57 10.83 3.93 1.43E-04 1.39E-02 0.85 TRIM52 0.23 8.12 3.91 1.51E-04 1.44E-02 0.80 ADAMTS9 0.34 7.78 3.91 1.53E-04 1.44E-02 0.79 PKD1 0.59 9.32 3.91 1.55E-04 1.44E-02 0.78 MBTPS1 0.33 8.16 3.91 1.55E-04 1.44E-02 0.78 SOHLH1 0.43 8.42 3.90 1.57E-04 1.44E-02 0.77 RIMS4 0.43 8.77 3.90 1.59E-04 1.45E-02 0.76 LPPR2 0.41 8.92 3.90 1.60E-04 1.45E-02 0.75 RAD9A 0.22 7.97 3.90 1.63E-04 1.46E-02 0.74 RFNG 0.42 9.60 3.89 1.64E-04 1.46E-02 0.73 PPP2R5B 0.27 9.40 3.89 1.66E-04 1.47E-02 0.72 SYMPK 0.39 8.14 3.88 1.72E-04 1.50E-02 0.68 ANGPT2 0.37 7.82 3.85 1.89E-04 1.61E-02 0.60 C19ORF28 0.21 7.92 3.84 1.97E-04 1.65E-02 0.57 CCDC109A 0.21 8.04 3.84 2.01E-04 1.67E-02 0.55 SERPING1 0.29 7.94 3.84 2.02E-04 1.67E-02 0.54 USP35 0.19 7.75 3.83 2.07E-04 1.70E-02 0.52 UNKL 0.30 8.12 3.82 2.13E-04 1.74E-02 0.49 LOC441268 0.37 8.82 3.82 2.14E-04 1.74E-02 0.49 PTP4A2 0.37 8.85 3.81 2.18E-04 1.75E-02 0.48 FLJ33534 0.21 7.73 3.81 2.18E-04 1.75E-02 0.48 BTBD9 0.30 7.95 3.81 2.24E-04 1.77E-02 0.45 ADCY6 0.29 8.67 3.80 2.30E-04 1.79E-02 0.43 TUFT1 0.24 8.15 3.80 2.31E-04 1.79E-02 0.42 ZFX 0.16 7.69 3.79 2.34E-04 1.80E-02 0.41 DDEFL1 0.30 8.49 3.79 2.41E-04 1.82E-02 0.38 UPF1 0.32 8.56 3.79 2.41E-04 1.82E-02 0.38 Genes 2018, 9, x FOR PEER REVIEW 14 of 39 CLASP1 0.33 10.32 3.78 2.45E-04 1.82E-02 0.37 YTHDF1 0.23 9.54 3.78 2.46E-04 1.82E-02 0.37 GCAT 0.33 8.73 3.78 2.48E-04 1.82E-02 0.36 LOC650580 0.33 7.82 3.78 2.50E-04 1.82E-02 0.35 PAPD5 0.39 8.76 3.77 2.52E-04 1.82E-02 0.35 MLL 0.21 8.28 3.77 2.55E-04 1.82E-02 0.33 LOC440359 0.41 7.93 3.76 2.60E-04 1.83E-02 0.32 TAOK2 0.38 8.23 3.75 2.70E-04 1.86E-02 0.28 TBC1D3G 0.23 7.90 3.75 2.72E-04 1.86E-02 0.28 TEF 0.46 9.72 3.75 2.77E-04 1.89E-02 0.26 SLC39A14 0.25 8.17 3.74 2.80E-04 1.89E-02 0.25 PTPRA 0.40 9.87 3.74 2.81E-04 1.89E-02 0.25 DLG4 0.72 11.79 3.74 2.85E-04 1.90E-02 0.23 COL6A1 0.41 8.61 3.73 2.91E-04 1.91E-02 0.22 TTC14 0.34 9.29 3.73 2.92E-04 1.91E-02 0.21 EDN1 0.44 7.95 3.73 2.96E-04 1.92E-02 0.20 MAPK11 0.38 8.41 3.72 3.08E-04 1.94E-02 0.17 COL11A1 0.30 8.27 3.72 3.10E-04 1.94E-02 0.16 LOC728452 0.25 7.75 3.72 3.11E-04 1.94E-02 0.16 IL18BP 0.27 7.98 3.71 3.12E-04 1.94E-02 0.15 RORB 0.50 9.14 3.71 3.16E-04 1.94E-02 0.14 CLIP2 0.42 9.71 3.71 3.17E-04 1.94E-02 0.14 DPP9 0.38 8.58 3.70 3.25E-04 1.95E-02 0.12 CHD8 0.40 9.67 3.70 3.31E-04 1.97E-02 0.10 HDAC5 0.30 8.42 3.70 3.31E-04 1.97E-02 0.10 NRBP1 0.21 7.86 3.70 3.33E-04 1.97E-02 0.10 FLJ37078 0.42 8.43 3.70 3.33E-04 1.97E-02 0.10 RPIA 0.23 8.12 3.69 3.37E-04 1.97E-02 0.09 FGF17 0.29 7.75 3.69 3.40E-04 1.97E-02 0.08 YWHAE 0.56 8.98 3.69 3.40E-04 1.97E-02 0.08 LOC613037 0.61 11.76 3.69 3.41E-04 1.97E-02 0.08 RANBP10 0.19 8.19 3.69 3.45E-04 1.98E-02 0.07 PBX1 0.17 7.72 3.68 3.55E-04 2.00E-02 0.04 Genes 2018, 9, x FOR PEER REVIEW 15 of 39 SCARA3 0.28 7.85 3.68 3.58E-04 2.00E-02 0.03 SYT7 0.48 10.48 3.67 3.59E-04 2.00E-02 0.03 MKL1 0.23 8.38 3.67 3.60E-04 2.00E-02 0.03 GADD45A 0.40 9.29 3.67 3.66E-04 2.01E-02 0.01 KLHL18 0.20 7.96 3.67 3.66E-04 2.01E-02 0.01 NAT9 0.20 8.76 3.67 3.66E-04 2.01E-02 0.01 NOL5A 0.36 9.96 3.66 3.71E-04 2.01E-02 0.00 TNFRSF25 0.56 9.30 3.66 3.72E-04 2.01E-02 0.00 KIAA0460 0.24 7.93 3.66 3.80E-04 2.03E-02 -0.02 AKAP8L 0.42 8.74 3.66 3.81E-04 2.03E-02 -0.02 MEG3 0.49 11.11 3.66 3.83E-04 2.04E-02 -0.03 ZBTB16 0.40 9.01 3.65 3.86E-04 2.05E-02 -0.04 GRB2 0.24 8.67 3.65 3.94E-04 2.08E-02 -0.05 BAT2 0.42 9.35 3.64 4.00E-04 2.08E-02 -0.07 KIAA0664 0.36 8.66 3.63 4.12E-04 2.13E-02 -0.09 TAOK1 0.37 8.33 3.63 4.15E-04 2.14E-02 -0.10 SS18L1 0.27 8.91 3.63 4.18E-04 2.14E-02 -0.11 ATG16L2 0.23 8.06 3.63 4.23E-04 2.14E-02 -0.11 LIMK1 0.30 8.10 3.62 4.36E-04 2.18E-02 -0.14 GOLGA8A 0.48 8.15 3.62 4.37E-04 2.18E-02 -0.14 RN7SK 0.49 8.04 3.62 4.41E-04 2.18E-02 -0.15 ICAM2 0.40 8.83 3.60 4.58E-04 2.23E-02 -0.19 MEIS3 0.27 7.95 3.60 4.61E-04 2.23E-02 -0.19 STK38 0.25 8.41 3.60 4.70E-04 2.25E-02 -0.21 MFHAS1 0.22 7.83 3.59 4.82E-04 2.30E-02 -0.23 TYW3 0.27 8.35 3.59 4.86E-04 2.31E-02 -0.24 LOC91461 0.26 8.22 3.58 4.91E-04 2.32E-02 -0.25 ATXN7L3 0.45 10.58 3.58 5.07E-04 2.35E-02 -0.28 MKNK1 0.29 8.81 3.57 5.08E-04 2.35E-02 -0.28 NUPR1 0.32 8.31 3.57 5.14E-04 2.36E-02 -0.29 FLJ25006 0.28 8.58 3.57 5.14E-04 2.36E-02 -0.29 COL23A1 0.18 7.85 3.56 5.25E-04 2.40E-02 -0.31 APLP1 0.63 10.04 3.56 5.26E-04 2.40E-02 -0.31 Genes 2018, 9, x FOR PEER REVIEW 16 of 39 ST5 0.29 8.04 3.56 5.30E-04