Published and copyright by: Medical Assotiation of Zenica-Doboj Canton; Address: Zenica, 72000, Bulevar kralja Tvrtka I 4, Bosnia and Herzegovina; tel./fax: +387 32 444 270; Email: [email protected], web site: http//www.ljkzedo.ba For ordering information please contact: Tatjana Žilo, [email protected]; Access to this journal is available free online trough: www.ljkzedo.com.ba The Journal is indexed by MEDLINE, EMBASE (Exerpta Medica), Scopus, EBSCO; ISSN 1840-0132 DTP by: Graphic and web design studio “B Panel” Zenica, Zmaja od Bosne bb, www.bpanel.ba, e-mail: [email protected], tel. +387 32 441 290, 441 291; Printed by: Graforad d.o.o. Zenica, Zmaja od Bosne bb, Zenica Tel.: 032 201-821; e-mail: [email protected] Medicinski Glasnik Official Publication of the Medical Association of Zenica-Doboj Canton Bosnia and Herzegovina EDITOR-IN-CHIEF Selma Uzunović, Zenica, Bosnia and Herzegovina MANAGING EDITOR Tarik Kapidžić, Zenica, Bosnia and Herzegovina EDITORS Solmaz Abdolrahimzadeh, Rome, Italy Luiz Ronaldo Alberti, Belo Horizonte, Brazil Mutay Aslan, Antalya, Turkey Adem Balić, Tuzla, Bosnia and Herzegovina Dubraka Bartolek, Zagreb, Croatia Branka Bedenić, Zagreb, Croatia Iva Christova, Sofia, Bulgaria Asja Čelebić, Zagreb, Croatia Josip Čulig, Zagreb, Croatia Filip Čulo, Zagreb, Croatia Jordan Dimanovski, Zagreb, Croatia Branko Dmitrović, Osijek, Croatia Davorin Đanić, Slavonski Brod, Croatia Ines Drenjančević, Osijek, Croatia Harun Drljević, Zenica, Bosnia and Herzegovina Mukaddes Esrefoglu, Istanbul, Turkey Ivan Fistonić, Zagreb, Croatia Roberta Granese, Messina, Italy Simona Gurzu, Tȋrgu Mureş, Romania Diane Medved Harper, Louisville, United State Lejla Ibrahimagić-Šeper, Zenica, Bosnia and Herzegovina Tatjana Ille, Ajman, United Arab Emirates Slobodan M. Janković, Kragujevac, Serbia Vjekoslav Jerolimov, Zagreb, Croatia Ioan Jung, Tȋrgu Mureş, Romania David Kovacevic, Yale University School of Medicine, New Haven, United States Sven Kurbel, Osijek, Croatia Snježana Pejičić, Banja Luka, Bosnia and Herzegovina Belma Pojskić, Zenica, Bosnia and Herzegovina Besim Prnjavorac, Tešanj, Bosnia and Herzegovina Asja Prohić, Sarajevo, Bosna Hercegovina Velimir Profozić, Zagreb, Croatia Radivoje Radić, Osijek, Croatia Amira Redžić, Sarajevo, Bosnia and Herzegovina Halima Resic, Sarajevo, Bosnia and Herzegovina Suad Sivić, Zenica, Bosnia and Herzegovina Sonja Smole-Možina, Ljubljana, Slovenia Vladimir Šimunović, Mostar, Bosnia and Herzegovina Ekaterine Tskitishvili, Liege, Belgium Aylin Türel Ermertcan, Manisa, Turkey Adrijana Vince, Zagreb, Croatia Jasmina Vraneš, Zagreb, Croatia EDITORIAL ASSISTANTS Hakija Bečulić, Zenica, Bosnia and Herzegovina Mersiha Mahmić-Kaknjo, Zenica, Bosnia and Herzegovina Secretary: Tatjana Žilo Proofreaders: Glorija Alić (English) Aras Borić (Bosnian, Croatian, Serbian) MEDICINSKI GLASNIK Official Publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina Volume 14, Number 2, August 2017 Free full-text online at: www.ljkzedo.com.ba, and www.doaj.org (DOAJ, Directory of Open Access Journals) Original 152 Cytotoxic and genotoxic studies of essential oil from Rosa damascene Mill., article Kashan, Iran Mohammad Shokrzadeh, Emran Habibi, Mona Modanloo 158 Significance of parathyroid scintigraphy and correlation of findings with parathyroid hormone values in patients undergoing hemodialysis Nermana Ahmetbegović, Nadira Suljagić, Vahidin Katica 164 The effect of functional insulin therapy on glycaemic parameters in children with diabetes mellitus type 1 Diana Štimjanin-Koldžo, Salem Alajbegović, Ena Štimjanin, Jasmina Mehinagić 169 Importance of inflammatory markers and IL-6 for diagnosis and follow up of patients with type 2 diabetes mellitus Maja Malenica, Mira Šilar, Tanja Dujić, Tamer Bego1, Sabina Semiz1, Selma Škrbo, Besim Prnjavorac, Adlija Čaušević 176 Metformin use associated with lower risk of cancer in patients with diabetes mellitus type 2 Jasna Kusturica, Aida Kulo Ćesić, Edis Gušić, Sanita Maleškić, Maida Rakanović- Todić, Damir Šečić 182 Efficacy of carbocisteine in the treatment of chronic obstructive pulmonary disease and impact on the quality of life Esad Alibašić, Amira Skopljak, Aida Čengić, Gorana Krstović, Nataša Trifunović, Tarik Čatić, Belma Kapo, Meliha Mehić, Amila Hadžimuratović 189 Attainment of gross motor milestones in children with Down syndrome in Kosovo - developmental perspective Samire Beqaj, Njomza Jusaj, Vujica Živković 199 Positron emission tomography/computed tomography (PET/CT) and CT for N staging of non-small cell lung cancer Sandra Vegar Zubović, Spomenka Kristić, Besima Hadžihasanović 204 Is post-treatment standardized uptake value a prognostic factor in unresectable non-small cell lung carcinoma? Ufuk Yılmaz, Zehra Yasar, Esra Korkmaz, Burcu Yalcın, Hakan Koparal, Engin Ozbilek 211 Nitric oxide biosynthesis during normal pregnancy and pregnancy complicated by preeclampsia Jasmin Hodžić, Sebija Izetbegović, Bedrana Muračević, Rasim Iriškić, Hana Štimjanin Jović 218 Socioeconomic and demographic factors associated with abdominal obesity in women of childbearing age Amira Kurspahić-Mujčić, Emir Zećo 224 Revision of the demographic and clinical data of patients with ulcerative colitis in Turkey Hatice Karagoz, Abdulsamet Erden, Hatice Karaman, Ahmet Karaman 229 The association between the serum levels of matrix metalloproteinase 9 and colorectal cancer Ismar Rašić, Azra Rašić, Goran Akšamija, Svjetlana Radović, Nedžad Šehović 236 Early results of the conservative treatment of distal radius fractures- immobilization of the wrist in dorsal versus palmar flexion Maki Grle, Miro Miljko, Ivana Grle, Faruk Hodžić, Tarik Kapidžić 244 Warfarin should not be used for thromboprohylaxis in elective major orthopaedic surgery: a Croatian perspective Robert Kolundžić, Marijana Šimić Jovičić, Maja Ðinkić, Tadija Petrović, Tomislav Crnković, Vladimir Trkulja 250 Croatian rotatory oblique three-dimensional osteotomy (CROTO) – a modified Wilson’s osteotomy for adult hallux valgus intended to prevent dorsal displacement of the distal fragment and to reduce shortening of the first metatarsal bone Robert Kolundžić, Mladen Mađarević, Vladimir Trkulja, Tomislav Crnković, Igor Šmigovec, Daniel Matek 257 Serum homocysteine levels in patients with probable vascular dementia Salem Alajbegović, Orhan Lepara, Almira Hadžović-Džuvo, Alma Mutevelić- Turković, Lejla Alajbegović, Asija Zaćiragić, Nesina Avdagić, Amina Valjevac, Nermina Babić, Amela Dervišević 262 Mental diseases and criminal offences committed by persons placed at the Forensic Department of the Penitentiary in Zenica, Federation of Bosnia and Herzegovina Edin Bjelošević, Adisa Krehmić, Halima Hadžikapetanović, Sanel Čoralić, Sonja Bjelošević 269 AUTHOR’S CORRECTION Medicinski Glasnik is indexed by MEDLINE, EMBASE (Exerpta Medica), EBSCO and Scopus ORIGINAL ARTICLE Cytotoxic and genotoxic studies of essential oil from Rosa damascene Mill., Kashan, Iran Mohammad Shokrzadeh1, Emran Habibi2, Mona Modanloo3 1Department of Toxicology-Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, 2Department of Phar- macognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences; Sari, Iran , 3Pharmaceutical Sciences Research Centre, Student research committe Mazandaran University of Medical Sciences, Sari, Iran ABSTRACT Aim Rosa damascene Mill. belongs to the family of Roseaceae and its essential oil is produced in large amounts in Iran. The wide application of rose oil has raised questions about potential adverse health effects. We have investigated cytotoxic activity and geno- toxic effects of Rosa oil from Kashan, Iran. Methods The cytotoxic effect and IC50 of the essential oil on the cell lines was studied followed by MTT assay. In this assay mi- Corresponding author: tochondrial oxidoreductase enzymes with reducing the tetrazolium Mona Modanloo dye MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) reflect the number of viable cells. Genotoxic effect of Pharmaceutical Sciences Research the oil was evaluated by micronucleus assay by evaluating produ- Center, Student research committee, ced micronuclei due to cytogenetic damage in binucleated lymp- Mazandaran University of Medical hocytes. Sciences, Results The results showed that essential oil significantly had Khazarabad Road, 48175-861 Sari, Iran; cytotoxic and genotoxic effects at doses over 10µg/mL (p<0.05). Phone: +98 911 1520 410; Also, essential oil of Rose showed lower IC50 in cancer cell line Fax: +981133543084; (A549) in comparison with the normal cell line (NIH3T3). Email: [email protected] Conclusion Cytotoxic and genotoxic properties of essential oil of Mohammad Shokrzadeh ORCID ID: http:// Rose in Kashan, Iran, are safe at a dose of 10µg/mL. Also, a good www.orcid.org/0000-0002-0071-6530 cytotoxic effect was shown and could be introduced as an antican- cer compound. Further studies are needed with regard to anti-can- cer effects of Rose essential oil. Original submission: 28 February 2017; Key words: micronucleus assay, MTT, Rose oil Revised submission: 15 March 2017; Accepted: 09 May 2017. doi: 10.17392/901-17 Med Glas (Zenica) 2017; 14(2): 152-157 152 Shokrzadeh et al. Rose oil cytotoxic and genotoxic effect INTRODUCTION MATERIALS AND METHODS Iran has a long history in cultivation and consump- Essential oil distillation tion of Rosa damascena, and it is known as an im- portant producer of rose oil in the world (1). Rosa Flowers were picked by hand before sunrise in damascena Mill. belongs