THE NATIONALUS009763454B2 AL MUTATTA MATRA UTTA TIK (12 ) United States Patent ( 10 ) Patent No. : US 9 , 763, 454 B2 Wachendorff- Neumann et al. ( 45 ) Date of Patent : Sep . 19 , 2017 (54 ) COMPOSITION COMPRISING A ( 56 ) References Cited BIOLOGICAL CONTROL AGENT AND A FUNGICIDE U . S . PATENT DOCUMENTS (71 ) Applicant: Bayer CropScience AG , Monheim 2014 /0005047 A1 * 1 /2014 Hungenberg .. .. A01N 43 /40 ( DE ) 504 / 100 ( 72 ) Inventors : Ulrike Wachendorff -Neumann , FOREIGN PATENT DOCUMENTS Neuwied (DE ) ; Peter Dahmen , Neuss WO 91 /02051 A1 2 / 1991 (DE ) ; Haruko Sawada , Langenfeld WO 2012 /013590 A2 2 / 2012 (DE ) WO 2012 /016989 A2 2 / 2012 WO 2012 / 072696 AL 6 / 2012 @( 73 ) Assignee : Bayer CropScience AG , Monheim (DE ) OTHER PUBLICATIONS @( * ) Notice : Subject to any disclaimer , the term of this Demirci, F ., et al. , “ Paecilomyces lilacinus, A Potential Biocontrol patent is extended or adjusted under 35 Agent on Apple Rust Mite Aculus, Schlechtendali and Interactions With Some Fungicides In Vitro , ” Phytoparasitica , Feb . 18 , 2010 , U . S . C . 154 (b ) by 0 days . vol. 38 , No . 2 , pp . 125 - 132 . Partridge , D . E . , et al . , “ Effect of Environmental Factors and Pesti (21 ) Appl. No .: 14 / 646 , 336 cides on Mycoparasitism of Sclerotinia minor by Coniothyrium minitans , ” Plant Disease , Nov. 1 , 2006 , vol. 90 , No . 11 , pp . (22 ) PCT Filed : Dec . 3 , 2013 1407 - 1412 . International Search Report and Written Opinion of the Interna ( 86 ) PCT No . : PCT/ EP2013/ 075351 tional Search Authority , International Application No . PCT / $ 371 ( C ) ( 1 ), EP2013/ 075351 , Jun . 17 , 2014 , 13 pages. ( 2 ) Date : May 20 , 2015 * cited by examiner ( 87) PCT Pub . No. : WO2014 /086764 Primary Examiner — Alton Pryor PCT Pub. Date : Jun . 12 , 2014 (74 ) Attorney , Agent, or Firm — Adam L . Lunceford ; Michelle L . Samonek (65 ) Prior Publication Data US 2015 /0320051 A1 Nov. 12 , 2015 ( 57 ) ABSTRACT The present invention relates to a composition comprising at ( 30 ) Foreign Application Priority Data least one biological control agent selected from the group consisting of Paecilomyces lilacinus strain 251 ( AGAL No . Dec. 3 , 2012 (EP ) .. 12195206 89 / 030550 ) and Coniothyrium minitans CON / M / 91 -08 (DSM 9660 ) and / or a mutant of these strains having all the (51 ) Int. Cl. identifying characteristics of the respective strain , and /or at AOIN 63 / 00 ( 2006 .01 ) least one metabolite produced by the respective strain that AOIN 25 /00 ( 2006 .01 ) exhibits activity against nematodes , insects and /or phyto AOIN 63 /04 ( 2006 . 01) pathogens , and at least one fungicide ( I ) selected from the AOIN 43 / 40 ( 2006 . 01 ) group consisting of inhibitors of the respiratory chain at (52 ) U . S . CI. complex I, II and III in a synergistically effective amount . CPC . .. .. AOIN 63/ 04 ( 2013. 01 ); AQIN 43/ 40 Furthermore , the present invention relates to a kit of parts ( 2013 .01 ) comprising said composition and the use of said composi (58 ) Field of Classification Search tion . None See application file for complete search history. 11 Claims, No Drawings US 9 , 763 ,454 B2 COMPOSITION COMPRISING A 91/ 02051 ) and has been deposited with the Australian Gov BIOLOGICAL CONTROL AGENT AND A ernment Analytical Laboratories ( AGAL ) in 1989 under the FUNGICIDE accession No. 89 /030550 . WO 2009 / 116106 realtes to the strain Trichoderma atro CROSS -REFERENCE TO RELATED 5 viride SC1 which is effective for biocontrol of fungal APPLICATION ( S ) diseases in plants . It has first been isolated from decayed hazelnut wood in northern Italy in 2000 and has been deposited at the “ Centraalbureeau voor Schimmelcultures” The present application is a 35 U . S . C . $ 371 national stage under the deposition number CBS No . 122089 in 2007 . entry of PCT/ EP2013 /075351 , filed on Dec . 3 , 2013 , which 10 A further known biological control agent is the strain claims priority of European Patent Application No . Coniothyrium minitans CON / M / 91 -08 ( cf . WO 96 /21358 ) 12195206 . 3 , filed on Dec. 3 , 2012 , which is hereby incor which has been deposited under the number DSM 9660 with porated by reference in its entirety . Applicant claims priority the German Collection ofMicroorganisms and Cell Cultures to each of the foregoing patent applications. The PCT in Braunschweig . It is used as a biological control against International Application was publishedshed in the English lan - 15 the fungal pathogens Sclerotinia sclerotiorum and Sclero guage . tinia minor ( causal agents of white mold on many plant The present invention relates to a composition comprising species ) . at least one biological control agent selected from specific In view of this , it was in particular an object of the present microorganisms and /or a mutant of these strains having all invention to provide compositions which exhibit activity the identifying characteristics of the respective strain , and / or 20 against insects , mites , nematodes and /or phytopathogens . at least one metabolite produced by the respective strain that Moreover, it was a further particular object of the present exhibits activity against insects , nematodes and /or phyto - invention , to reduce the application rates and broaden the pathogens and at least one specified fungicide ( I ) in a activity spectrum of the biological control agents and fun synergistically effective amount. Furthermore , the present gicides , and thereby to provide a composition which , pref invention relates to the use of this composition as well as a 25 erably at a reduced total amount of active compounds method for reducing overall damage of plants and plant applied , has improved activity against insects , mites , nema parts . todes and / or phytopathogens . In particular , it was a further Synthetic insecticides or fungicides often are non - specific object of the present invention to provide a composition and therefore can act on organisms other than the target which , when applied to a crop , results in a decreased amount ones , including other naturally occurring beneficial organ - 30 of residues in the crop , thereby reducing the risk of resis isms. Because of their chemical nature , they may be also tance formation and nevertheless provides efficient disease toxic and non -biodegradable . Consumers worldwide are control. increasingly conscious of the potential environmental and Accordingly , it was found that these objects at least partly health problems associated with the residuals of chemicals , are solved by the compositions according to the invention as particularly in food products . This has resulted in growing 35 defined in the following . The composition according to the consumer pressure to reduce the use or at least the quantity present invention preferably fulfills the above- described of chemical ( i. e . synthetic ) pesticides . Thus, there is a need needs . It has been surprisingly discovered that the applica to manage food chain requirements while still allowing tion of the composition according to the present invention in effective pest control. a simultaneous or sequential way to plants , plant parts , A further problem arising with the use of synthetic 40 harvested fruits , vegetables and /or plant' s locus of growth insecticides or fungicides is that the repeated and exclusive preferably allows better control of insects , mites , nematodes application of an insecticide or fungicides often leads to and / or phytopathogens than it is possible with the strains , selection of resistant microorganisms . Normally , such their mutants and / or their metabolites produced by the strains are also cross - resistant against other active ingredi- strains on the one hand and with the individual fungicides on ents having the samemode of action . An effective control of 45 the other hand , alone ( synergistic mixtures ) . By applying the the pathogens with said active compounds is then not biological control agent and the fungicide according to the possible any longer . However, active ingredients having new invention the activity against insects , mites , nematodes mechanisms of action are difficult and expensive to develop and / or phytopathogens is preferably increased in a super The risk of resistance development in pathogen popula - additive manner . Preferably , the application of the compo tions as well as environmental and human health concerns 50 sition according to the invention induces an increase in the have fostered interest in identifying alternatives to synthetic activity of phytopathogens in a superadditive manner . insecticides and fungicides for managing plant diseases . The As a consequence , the composition according to the use of biological control agents (BCAs ) is one alternative . present invention preferably allows a reduced total amount However, the effectiveness of most BCAs is not at the same of active compounds to be used and thus the crops which level as for conventional insecticides and fungicides , espe - 55 have been treated by this composition preferably show a cially in case of severe infection pressure . Consequently , decreased amount of residues in the crop . Accordingly , the known biological control agents , their mutants and metabo - risk of resistance formation of harmful microorganisms is lites produced by them are, in particular in low application decreased . rates , not entirely satisfactory . The present invention is directed to a composition com Thus, there is a constant need for developing new , alter - 60 prising at least one