2nd International Symposium on Bio-nanosystems October 31 – November 2, 2008 Koshiba Hall, the University of Tokyo, Hongo, Tokyo, Japan Program and Abstracts The Symposium is hosted by MEXT, Scientific Research on Priority Areas: Bio-nanosystems (http://nanobio.phys.s.u-tokyo.ac.jp/NanoSystem/) Correspondence: Hideo Higuchi, Ph.D. Department of Physics, Graduate School of Science, the University of Tokyo 7-3-1 Hongo Bunkyou-ku Tokyo, Japan 113-0033 Phone: +81-35841-4128 Fax: +81-35841-7646 e-mail: [email protected] i October 31st (i ) October 31st (Friday) Welcome Address 13:00-13:05 Hideo Higuchi (University of Tokyo) Session I. Actomyosin nanosystem (Session Chair: Motoshi Kaya) 13:05-13:35 Motoshi Kaya (University of Tokyo) S1-1 Introduction Power stroke distance and load-dependent step size of single skeletal myosins 13:35-14:05 So Nishikawa (Osaka University) S1-2 Single molecule detection of two-legged nano-machines 14:05-14:45 Ronald Rock (University of Chicago) S1-3 Navigating the Cytoskeleton with Myosins 14:45-15:15 Issei Mabuchi (Gakusyuin University) S1-4 Structure and assembly of actin cytoskeletons in fission yeast cells 15:15-15:30 Takeyuki Wakabayashi (Teikyo University) S1-5 Tropomyosin-binding motifs of actin 15:30-15:45 Taro Uyeda (AIST) S1-6 A novel expression system for toxic mutant actins, and characterization of mutant actins defective in motility. 15:45-16:00 Coffee Break 16:00-18:00 Poster Session 1 ii November 1st. (Satday November 1st (Saturday) ) Session II. Structure and Mechanism of Dynein (Session Chair: Kazuhiro Oiwa) 9:00-9:05 Kazuhiro Oiwa (NICT) Introduction 9:05-9:40 Stan Burgess (University of Leeds) S2-1 Structure and mechanism of dynein studied by electron microscopy 9:40-10:05 Keiko Hirose (AIST) S2-2 Structural changes in dynein molecules bound to microtubules 10:05-10:40 Daniela Nicastro (Brandeis University) S2-3 Three-dimensional structure of cytoplasmic and axonemal dynein bound to microtubules 10:40-10:55 Yusuke Kato (University of Leeds) S2-4 Structure of the microtubule-binding domain of a flagellar dynein 10:55-11:10 Coffee Break 11:10-11:35 Takahide Kon (University of Tokyo) S2-5 Structure and mechanochemical cycle of cytoplasmic dynein 11:35-11:50 Shiori Toba (NICT) S2-6 Mechanical and enzymatic properties of the heterodimeric axonemal dynein-f 11:50-12:05 Kenji Imamula (University of Tokyo) S2-7 The stalk coiled coil of axonemal dynein is responsible for microtubule rotation 12:05-12:20 Yuki Hirota (Nagoya City University) S2-8 Polarization of ependymal cilia during mouse brain development 12:20-13:50 Lunch (Put up posters in Poster session 2) Session III. Dynein in Axonemes and Cells (Session Chair, Kazuhiro Oiwa) 13:50-14:20 Takashi Ishikawa (ETH Zürich) S2-9 Structural study of outer and inner dynein arms by electron cryo-tomography 14:20-14:45 Shinji Kamimura (Chuo University) S2-10 Dynamic features of the axonemal structure of sea-urchin sperm flagella revealed by small-angle X-ray diffraction analysis 14:45-15:20 Yoko Toyoshima (University of Tokyo) S2-11 Reguration of dynein motility by LIS1 and NDEL1 15:20-15:45 Erika Holzbaur (University of Pennsylvania) S2-12 Flexibility in Dynein Motor Function at Obstacles and Intersections 15:45-16:00 Coffee Break 16:00-18:00 Poster Session 2 18:30-20:30 Conference Dinner iii November 2 November 2nd (Sunday) Session IV. Kinesin-microtubule nanosystem (Session Chair: Michio Tomishige) 9:00-9:30 Michio Tomishige (University of Tokyo) S3-1 Introduction Single molecule observation of conformational changes of kinesin 9:30-10:05 Hernando Sosa (Albert Einstein College of Medicine) S3-2 The kinesin-1 walking mechanism as revealed by ensemble and single-molecule fluorescence polarization microscopy 10:05-10:25 Coffee Break 10:25-11:00 Thomas Surrey (EMBL) S3-3 Microtubule plus-end tracking by EB1 and CLIP-170: Mechanistic insight from an in vitro reconstitution 11:00-11:30 Gohta Goshima (Nagoya University) S3-4 Microtubule generation within the mitotic spindle 11:30-11:45 Ayumu Yamamoto (Shizuoka University) S3-5 Meiotic telomere clustering depends on mircrotubule motors in fission yeast. 11:45-12:00 Naofumi Handa (University of Tokyo) S3-6 Genomic ID sequence recognition by a genome maintenance enzyme 12:00-13:20 Lunch Session V. Bio-nanosystems from Molecules to Cells (Session Chair: Hideo Higuchi) 13:20-14:00 Keiichi Namba (Osaka University) S3-7 Molecular Mechanisms of Self-Assembly and Its Regulation of the Bacterial Flagellum 14:00-14:30 Chikako Shingyoji (University of Tokyo) S3-8 Roles of motor proteins in the regulation of melanophore responses in zebrafish 14:30-15:00 Hideo Higuchi (University of Tokyo) S3-9 Myosin, kinesin and dynein in in vitro and cells iv November 2 Poster Session Poster Session 1 (October 31st) 16:00-18:00 P1-1 Takeshi Nakagawa, Hiroto Tanaka, Kazuo Sasaki (Tohoku University) Why can the energy consumption of myosin be reduced when they work together? P1-2 Atsuko H. Iwane, Takako Ichinose, Masatoshi Nishikawa, Tomotaka Komori (Osaka University) The effect of the Magnesium ion in the ATPase activity and motility of MyosinVa P1-3 Masayuki Takahashi, Takashi Nakasawa, Masaaki Sato, Yuusuke Kawashima, Fumiko Matsuzawa, Sei-ichi Aikawa, Michio Yazawa (Hokkaido University) Charge clusters in the C-terminal rod region are essential for assembly of vertebrate nonmuscle myosin II P1-4 Shigehiko Yumura, Atushi Yata, Akira Nagasaki, Yoshiaki Iwadate (Yamaguchi University) Dynamics of myosin II filaments and myosin II heavy chain kinase C in live cells under TIRF microscopy P1-5 Kazuhiro Kawanoue, Shigeru Chaen, Shinsaku Maruta (Soka University) Interaction of ATP driven motor proteins with ATP analogue having syn conformation with respect to the adenine-ribose bond P1-6 Hideki Shishido, Masafumi D. Yamada, Kazunori Kondo, Shinsaku Maruta (Soka University) Photocontrol of Calmodulin Function using Photochromic Compound P1-7 Ryoki Ishikawa, Ayumi Takahashi, Akio Nakamura, Kazuhiro Kohama (Gunma University) In vitro sliding on myosin II of F-actin under the conditions fully saturated with variety of actin-binding proteins P1-8 Masak Takaine, Osamu Numata, Kentaro Nakano (University of Tsukuba) The fission yeast IQGAP, Rng2 is a monomeric actin-bundling protein required for contractile ring formation P1-9 Shin-ichiro Kojima, Yvonne Aratyn, Danijela Vignjevic, Thomas Schaus (Northwestern University) Molecular basis of actin-bundling during filopodia protrusion. P1-10 Masahiko Harata , Naoki Aoyama, Asako Oka, Kumiko Kitayama, Susan Gasser, Kenji Shimada, Yukako Oma (Tohoku University) Actin-related proteins involved in genome and chromosome functions P1-11 Hiroaki Kubota, Sergey V. Mikhailenko, Shin’ichi Ishiwata (Waseda University ) D-loop of actin is important for the motility of myosins II and V v P1-12 Eri Nishihara, Makoto Tominaga, Hiroyuki Nakagawa (Fukuoka University) Neurite elongation from cultured insect neural cell P1-13 Hideki Nakanishi, Seisuke Arai, Tunehito Higashi, Ikuo Wada (Fukushima Medical University) Aberrant diffusion of secretory proteins in the endomembranes P1-14 Keisuke Ueda, Chieko Kimura, Masao Miki, Toshiaki Arata (Osaka University) Distribution of distance between spin-labeled tropomyosin and actin in reconstituted skeletal muscle thin filament by SDSL-ESR study P1-15 Kensaku Mizuno, Tai Kiuchi, Souichi Kurita, Kazumasa Ohashi (Tohoku University) Role of cofilin in actin filament assembly and disassembly during lamellipodium formation and extension P1-16 Kentaro Nakano and Masak Takaine (University of Tsukuba) Functional analysis of a novel ADF/cofilin-super family protein involved in the organization of actin cytoskeleton in fission yeast P1-17 Sinji Yoshiyama, Ryoki Ishikawa, Akio Nakamura, Sugie Higashi-Fumime, Hideyo Takatsuki, Kevin Rice, Eric. R.Blough, Kazuhiro Kohama (Gunma University) Actin-bundles as a transporter of nano-sized cargoes. P1-18 Togo Shimozawa and Shin’ichi Ishiwata (University of Tokyo) Detection of the structural distortion in a single actin filament induced by tensile force using fluorescence microscopy P1-19 Hironori Ueno, Takuo Yasunaga, Chikako Shingyoji and Keiko Hirose (AIST) Structural analysis of outer arm dynein molecules bound to microtubules P1-20 Takuo Yasunaga, Hiroko Takazaki, Yoshinori Ohta (Kyushu Institute of Technology) Image analysis systems from electron micrographs of motor proteins and their complexes P1-21 Izumi Nakano and Chikako Shingyoji (University of Tokyo) Effects of ATP-γS on the microtubule-binding and the motile activity of 21S dynein of sea urchin sperm flagella at high ATP P1-22 Yumi Watanabe and Chikako Shingyoji (University of Tokyo) Conditions for the induction of continuous beating by externally applied bending in demembranated motionless sea urchin sperm flagella at low ATP P1-23 Hitoshi Sakakibara, Shiori Toba, Hiroyuki Iwamoto, Kazuhiro Oiwa (NICT) Helical Rearrangement of the Axonemal Components in Chlamydomonas flagella coupled with Ca2+ concentrations revealed by X-ray Fiber Diffractions vi P1-24 Katsutoshi Mizuno, Yuji Shitaka, Hitoshi Sakakibara, Kazuhiro Oiwa, Kazuo Inaba (University of Tsukuba) Calaxin is a potential Ca2+-dependent modulator of outer arm dynein P1-25 Kazuo Inaba, Mamoru Nomura, Akiko Hozumi, Lihong Zhu, Mia Nakachi (University of Tsukuba) Molecular signaling for the activation of flagella motility in the ascidian Ciona intestinalis P1-26 Kogiku Shiba, Shoji A Baba, Manabu Yoshida (University of Tokyo) Mechanism of sperm chemotaxis in the ascidian: When do spermatozoa detect the chemoattractant? P1-27 Toshiki Yagi, Keigo Uematsu, Zhongmei Liu, Ritsu Kamiya (Kyoto University) Identification of three novel dyneins localized only to the proximal region of Chlamydomonas flagella P1-28 Kazuo Sasaki and Hidemi Akiyama (Tohoku University) A unified theoretical model for “bipedal” molecular motors Poster Session 2 (November 1st) 16:00-18:00 P2-1 M. D. Yamada, T. Aihara, K. Sugata, P. G. Fajer, M. Miki, S. Maruta, Toshiaki Arata (Osaka University) Conformational dynamics of unique elements of kinesin and troponin observed by spin-labeling ESR spectroscopy P2-2 Satoshi Yasuda, Masafumi D.