Electronic Supplementary Material (ESI) for Metallomics This journal is © The Royal Society of Chemistry 2013 1 Supporting Figure Legend 2 3 Figure S1: RPS4Y1 mRNA levels. Levels of this mRNA were measured as part of the validation of Affymetrix gene expression data from DEG.C. Cclones are controls (untreated 4 Beas-2B that grew in soft agar) and Vclones are clones transformed by chronic NaVO3 exposure. 5 6 Figure S1 7 8 9 Table S1: Differentially expressed genes in transformed clones (DEG.C). Gene Symbol Gene Description Adjusted Fold p-value change TMEM47 transmembrane protein 47 1.9E-02 36.196 SPTLC3 serine palmitoyltransferase, long chain base subunit 3 7.8E-03 33.565 RPS4Y1 ribosomal protein S4, Y-linked 1 6.9E-03 28.724 EYA4 eyes absent homolog 4 (Drosophila) 3.9E-03 18.298 EIF1AY eukaryotic translation initiation factor 1A, Y-linked 8.6E-03 13.872 EDIL3 EGF-like repeats and discoidin I-like domains 3 1.3E-02 12.147 DDX3Y DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked 3.9E-03 12.110 UTY ubiquitously transcribed tetratricopeptide repeat gene, Y-linked 9.5E-03 10.134 CYP1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 2.2E-02 9.194 CD24 CD24 molecule 1.4E-02 8.328 1 Electronic Supplementary Material (ESI) for Metallomics This journal is © The Royal Society of Chemistry 2013 GABRA3 gamma-aminobutyric acid (GABA) A receptor, alpha 3 1.7E-02 7.643 ZNF730 zinc finger protein 730 2.0E-02 7.283 RARB|LOC100130354 retinoic acid receptor, beta | hypothetical protein LOC100130354 3.3E-02 7.168 DNER delta/notch-like EGF repeat containing 3.6E-02 7.067 SERPINB2|SERPINB10 serpin peptidase inhibitor, clade B (ovalbumin), member 2 | serpin peptidase inhibitor, clade B (ovalbumin), 1.6E-02 6.863 member 10 USP9Y ubiquitin specific peptidase 9, Y-linked 7.8E-03 6.194 PPAPDC1A phosphatidic acid phosphatase type 2 domain containing 1A 1.6E-02 5.853 HLF hepatic leukemia factor 4.3E-02 5.837 ASAM adipocyte-specific adhesion molecule 2.0E-02 5.425 ABI3BP ABI family, member 3 (NESH) binding protein 4.5E-02 5.381 ZFY zinc finger protein, Y-linked 1.8E-02 5.288 NTN4 netrin 4 2.2E-02 5.284 ZNF300 zinc finger protein 300 1.4E-02 4.987 WNT5A wingless-type MMTV integration site family, member 5A 1.7E-02 4.967 COL5A2 collagen, type V, alpha 2 2.1E-02 4.931 MGAM maltase-glucoamylase (alpha-glucosidase) 3.1E-02 4.716 ZNF280B zinc finger protein 280B 2.1E-02 4.649 F2RL2 coagulation factor II (thrombin) receptor-like 2 2.9E-02 4.600 SRPX2 sushi-repeat-containing protein, X-linked 2 9.9E-03 4.590 PDE1C phosphodiesterase 1C, calmodulin-dependent 70kDa 1.4E-02 4.495 PRICKLE1 prickle homolog 1 (Drosophila) 3.4E-02 4.390 CYorf15A chromosome Y open reading frame 15A 1.5E-02 4.302 LGR4 leucine-rich repeat-containing G protein-coupled receptor 4 1.1E-02 4.015 GLIS3 GLIS family zinc finger 3 1.1E-02 3.998 NRP1 neuropilin 1 1.9E-02 3.990 IL31RA interleukin 31 receptor A 3.6E-02 3.907 CRISPLD2 cysteine-rich secretory protein LCCL domain containing 2 3.6E-02 3.815 SLC4A4 solute carrier family 4, sodium bicarbonate cotransporter, member 4 1.9E-02 3.793 SNAI2 snail homolog 2 (Drosophila) 1.8E-02 3.733 MEIS1 Meis homeobox 1 1.3E-02 3.720 2 Electronic Supplementary Material (ESI) for Metallomics This journal is © The Royal Society of Chemistry 2013 SPOCK1 sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 2.4E-02 3.654 MMP16 matrix metallopeptidase 16 (membrane-inserted) 1.8E-02 3.643 TGFA transforming growth factor, alpha 2.3E-02 3.607 ZNF717|LOC100287163 zinc finger protein 717 | similar to Zinc finger protein 717 7.8E-03 3.590 TNC tenascin C 8.0E-03 3.522 FHDC1 FH2 domain containing 1 1.1E-02 3.496 PPP1R3C protein phosphatase 1, regulatory (inhibitor) subunit 3C 1.9E-02 3.474 SNORD13 small nucleolar RNA, C/D box 13 2.5E-02 3.348 ADAM12 ADAM metallopeptidase domain 12 3.4E-02 3.324 LOC554202 microRNA 31 1.5E-02 3.317 NTF3 neurotrophin 3 3.4E-02 3.314 NEGR1 neuronal growth regulator 1 3.6E-02 3.312 SNORD13|RNU13P3 small nucleolar RNA, C/D box 13 | RNA, U13 small nuclear pseudogene 3 2.5E-02 3.303 SH3RF2 SH3 domain containing ring finger 2 1.8E-02 3.296 MPP4 membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) 1.7E-02 3.168 SLC8A1 solute carrier family 8 (sodium/calcium exchanger), member 1 3.3E-02 3.157 CD109 CD109 molecule 3.8E-02 3.131 KDM5D lysine (K)-specific demethylase 5D 7.8E-03 3.108 MAMLD1 mastermind-like domain containing 1 8.6E-03 3.059 DUSP4 dual specificity phosphatase 4 1.5E-02 3.043 TES testis derived transcript (3 LIM domains) 1.8E-02 3.022 SLC16A2 solute carrier family 16, member 2 (monocarboxylic acid transporter 8) 8.1E-03 2.976 G0S2 G0/G1switch 2 3.6E-02 2.952 PRICKLE2 prickle homolog 2 (Drosophila) 1.7E-02 2.951 GLT8D4 glycosyltransferase 8 domain containing 4 1.1E-02 2.919 KIAA1217 sickle tail protein homolog 2.2E-02 2.855 MST131 MSTP131 2.1E-02 2.854 SYTL2 synaptotagmin-like 2 2.0E-02 2.833 SHISA2 shisa homolog 2 (Xenopus laevis) 2.1E-02 2.822 KCTD4 potassium channel tetramerisation domain containing 4 3.6E-03 2.771 ITGBL1 integrin, beta-like 1 (with EGF-like repeat domains) 2.4E-03 2.697 3 Electronic Supplementary Material (ESI) for Metallomics This journal is © The Royal Society of Chemistry 2013 GPR39|LYPD1 G protein-coupled receptor 39 | LY6/PLAUR domain containing 1 3.1E-02 2.664 ARRDC3 arrestin domain containing 3 2.2E-02 2.654 LAMB3 laminin, beta 3 2.8E-02 2.641 CHST11 carbohydrate (chondroitin 4) sulfotransferase 11 3.7E-02 2.618 FOXD1 forkhead box D1 1.0E-02 2.604 PTPRS protein tyrosine phosphatase, receptor type, S 4.4E-02 2.555 GPR56 G protein-coupled receptor 56 2.3E-02 2.551 SATB2 SATB homeobox 2 3.0E-02 2.548 TOX2 TOX high mobility group box family member 2 1.5E-02 2.531 EMP1 epithelial membrane protein 1 2.4E-02 2.525 BACE1 beta-site APP-cleaving enzyme 1 4.9E-02 2.521 CORO2B coronin, actin binding protein, 2B 2.3E-02 2.491 MID1 midline 1 (Opitz/BBB syndrome) 1.5E-02 2.484 PTGER2 prostaglandin E receptor 2 (subtype EP2), 53kDa 2.3E-02 2.483 ANTXR2 anthrax toxin receptor 2 1.3E-02 2.459 OPN3|KMO opsin 3 | kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) 8.6E-03 2.442 ABLIM3 actin binding LIM protein family, member 3 2.7E-02 2.435 INPP4B inositol polyphosphate-4-phosphatase, type II, 105kDa 4.7E-02 2.434 LRIG1 leucine-rich repeats and immunoglobulin-like domains 1 2.6E-02 2.426 ZNF608 zinc finger protein 608 2.3E-02 2.388 C5orf13 chromosome 5 open reading frame 13 1.9E-02 2.374 HPCAL1 hippocalcin-like 1 2.0E-02 2.352 CYorf15B chromosome Y open reading frame 15B 4.1E-02 2.350 TGFBR2 transforming growth factor, beta receptor II (70/80kDa) 1.2E-02 2.331 CDH4 cadherin 4, type 1, R-cadherin (retinal) 3.6E-02 2.331 NRIP1 nuclear receptor interacting protein 1 1.8E-02 2.322 PER3|UTS2 period homolog 3 (Drosophila) | urotensin 2 1.5E-02 2.317 FAM172A family with sequence similarity 172, member A 3.3E-02 2.309 TUBA1A tubulin, alpha 1a 3.4E-02 2.266 LY6K lymphocyte antigen 6 complex, locus K 2.5E-02 2.231 PNPLA3 patatin-like phospholipase domain containing 3 2.7E-02 2.219 4 Electronic Supplementary Material (ESI) for Metallomics This journal is © The Royal Society of Chemistry 2013 SEMA7A semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) 3.7E-02 2.209 ATL1 atlastin GTPase 1 2.6E-02 2.209 ITFG1 integrin alpha FG-GAP repeat containing 1 2.0E-02 2.204 MARVELD2 MARVEL domain containing 2 4.4E-02 2.203 HOXB2 homeobox B2 2.4E-02 2.203 JAG1 jagged 1 (Alagille syndrome) 8.1E-03 2.197 MARVELD2 MARVEL domain containing 2 4.5E-02 2.196 MMP14 matrix metallopeptidase 14 (membrane-inserted) 4.5E-02 2.173 STC2 stanniocalcin 2 2.6E-02 2.168 PIK3R3 phosphoinositide-3-kinase, regulatory subunit 3 (gamma) 1.5E-02 2.167 SPOCD1 SPOC domain containing 1 2.0E-02 2.165 ATG10 autophagy related 10 2.7E-02 2.149 NRG1 neuregulin 1 3.1E-02 2.144 S1PR1 sphingosine-1-phosphate receptor 1 1.5E-02 2.140 PRKY protein kinase, Y-linked 8.0E-03 2.113 LAMB1 laminin, beta 1 3.2E-02 2.111 AOX1 aldehyde oxidase 1 8.6E-03 2.099 SERPINE2 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 4.3E-02 2.093 PLCE1 phospholipase C, epsilon 1 4.4E-02 2.093 CUZD1 CUB and zona pellucida-like domains 1 2.8E-02 2.086 FN1 fibronectin 1 2.0E-02 2.083 ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) 1.1E-02 2.083 DYSF dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) 4.1E-02 2.061 ADA adenosine deaminase 2.2E-02 2.052 VDR vitamin D (1,25- dihydroxyvitamin D3) receptor 1.4E-02 2.047 SLC6A8|SLC6A10P solute carrier family 6 (neurotransmitter transporter, creatine), member 8 | solute carrier family 6 2.0E-02 2.043 (neurotransmitter transporter, creatine), member 10 (pseudogene) GDF15 growth differentiation factor 15 4.9E-02 2.040 ECM1 extracellular matrix protein 1 1.6E-02 2.034 LHFPL2 lipoma HMGIC fusion partner-like 2 4.3E-02 2.027 CPEB2 cytoplasmic polyadenylation element binding protein 2 2.7E-02 2.025 5 Electronic Supplementary Material (ESI) for Metallomics This journal is © The Royal Society of Chemistry 2013 TMEM14A transmembrane protein 14A 1.7E-02 2.024 HOMER2 homer homolog 2 (Drosophila) 4.0E-02 2.022 PHLDA1 pleckstrin homology-like domain, family A, member 1 4.8E-02 2.017 TSPAN15 tetraspanin 15 2.4E-02 2.014 ARHGAP18 Rho GTPase activating protein 18 4.8E-02 2.014 SLC6A8|SLC6A10P solute carrier family 6 (neurotransmitter transporter, creatine), member 8 | solute carrier family 6 2.2E-02 2.004 (neurotransmitter transporter, creatine), member 10 (pseudogene) LRP1 low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor) 1.8E-02 1.999