Ultra Rainbow Quantitative Particle Kit Multicolor Flow Cytometry Fluorescence Standard Contains 6 intensities of Ultra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laser-based CCD fluorimeter Provides microsphere reference standards for fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC) and Pacific Blue (PB) fluorescent channels using four different NIST reference fluorophores; fluorescein, Nile Red, APC and Coumarin 30. Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications. Histograms of Cat. No. URQP-38-6K - UltraRainbow Quantitative Particle Kit, 3.9µm, 6 Intensities To learn more about Spherotech Ultra Rainbow Quantitative Particles visit us online SPHEROTM Ultra Rainbow Quantitative Particle Kit Designed to determine the accuracy, precision & sensitivity in the Pacific Blue, FITC, PE, & APC channels Accuracy Provides a bead set to accurately measure comparable data from different types of instruments at different locations Determines the linearity of the digital output from the analog-to-digital converter Precision Detects laser fluctuations, failed electronics, or obstructions in the flow cell Sensitivity Determines background noise level and instrument efficiency Checks instrument's ability to distinguish dim peaks from blank beads Detects dye or light contamination URQP-38-6K MFI vs. ERF Value Assigned 1E7 Equation y = a + b*x Equation y = a + b*x Weight No Weighting Weight No Weighting Residual Sum of 0.01242 Residual Sum of 0.00147 Squares Squares Adj. R-Square 0.99543 Adj. R-Square 0.99929 100000 Value Standard Error Value Standard Error APC Intercept 0.65201 0.12683 PE Intercept 2.26338 0.05189 APC Slope 0.98102 0.03322 PE Slope 0.9552 0.01271 1000000 10000 ERF APC ERF NR 100000 1000 1000 10000 100000 MFI PE MFI APC 1E7 Equation y = a + b*x 1000000 Equation y = a + b*x Weight No Weighting Weight No Weighting Residual Sum of 0.02101 Residual Sum of 6.5796E-4 Squares Squares Adj. R-Square 0.99044 Adj. R-Square 0.99968 Value Standard Error Value Standard Error DAPI Intercept 2.05065 0.19413 FITC Intercept 1.31119 0.0352 DAPI Slope 0.97553 0.04787 FITC Slope 0.95309 0.00857 1000000 100000 ERF C30 ERF FITCERF 100000 10000 1000 10000 100000 1000 10000 100000 MFI DAPI MFI FITC MFI FITC ERF FITC MFI PE ERF NR MFI APC ERF APC MFI DAPI ERF C30 528 8020 483 64900 310 1110 487 52400 5268 71000 4842 653000 1475 6480 4250 323000 15255 209000 14354 1.71E6 4146 18000 12385 995000 41182 499000 40108 4.41E6 22942 73300 35058 3.83E6 86038 1.03E6 80739 8.93E6 89421 331000 88138 7.17E6 Particle Type and Surface Size, µm Catalog No. Unit Ultra Rainbow Quantitative Particle Kit, 6 Intensities, 2E6/mL, 3.5-3.9 URQP-38-6K 6x1mL (NIST Traceable ERF Flow Cytometry Standard) Visit us online for more details regarding Spherotech Ultra Rainbow Quantitative Particle Kit.