EPIGENOMICS CORE The Epigenomics Core provides sample quality control, library preparation and sequencing for the study of cytosine and histone modifications. Products & Services We offer a wide variety of services in epigenomics and bioinformatics resources, including: • Genome-wide DNA Methylation Profiling ❍ Whole-genome bisulfite sequencing: whole-genome base-pair resolution quantification of DNA methylation ❍ ERRBS (Enhanced Reduced Representation Bisulfite Sequencing): Quantitative base-pair measurement of cytosine methylation at ~3M CpGs genome-wide located at promoters, CpG islands and shores, and intergenic regulatory regions ❍ meDIP-seq: Antibody-based method for identification of mC-enriched areas across the genome. ❍ Support with data analysis • Targeted (locus-specific) DNA Methylation ❍ Primer design consultation and training ❍ Bisulfite conversion of DNA and PCR amplification of target sequences ❍ MassARRAY EpiTYPER sequencing ❍ Pyrosequencing ❍ Support with data analysis • Chromatin Modification: ChIP-Seq ❍ Library preparation of user-provided immunoprecipitated DNA ChIP-seq data analysis ❍ Histone Modification by ChIP-Seq (limited initial availability) • Hydroxymethylation ❍ Genome-wide and Target Specific Epigenomics Core Email the Core to set up a consultation, including a FREE initial consultation to scope Biomedical Research Core Facilities your project and ascertain how best to your meet your specific project needs. MSRB II 2568 1150 W. Medical Center Drive Ann Arbor, MI 48109-0674 OVER Coming soon to the Core • Chromatin Accessibility Cost Estimates & Fees About the BRCF For project-specific costs and fees, please contact the Core. The BRCF, part of the University of Michigan Medical School Office of Research, is a collection of centralized labs and services offering state-of-the- art instruments, resources and expertise to biomedical researchers, investigators and educators. For more information on the BRCF, visit research.med.umich.edu/brcf From top-notch library preparation and sample quality controls to expert analysis, the Epigenomics Core strives to offer the best in epigenomics and bioinformatics resources. – Claudia Lalancette, Ph.D., Managing Director, Epigenomics Core CONTACT US Dana Dolinoy Hours & Location Ph.D., Faculty Director Open Monday-Friday BIOMEDICAL RESEARCH CORE FACILITIES 8:30 a.m.- 4:30 p.m. Claudia Lalancette University of Michigan Medical School MSRB II 2568 Ph.D., Managing Director 1150 W. Medical Center Drive 2570 MSRB II • 1150 W. Medical Center Drive 734-764-2223 Ann Arbor, MI 48109-0674 Ann Arbor, MI 48109-5674 [email protected] 734-647-4776 Maureen Sartor [email protected] Ph.D., Faculty Bioinformatics Director research.med.umich.edu/ epigenomicscore Karthik R Padmanabhan Ph.D., Senior Bioinformatics Analyst Sonoko Natsume Senior Research Lab Technician.